EC Number | Application | Comment | Organism |
---|---|---|---|
1.1.1.86 | drug development | the enzyme is a promising target for the design of herbicides | Oryza sativa |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.86 | overexpression in Escherichia coli strain BL21(DE3) | Oryza sativa |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.86 | 2-dimethylphosphinoyl-2-hydroxyacetate | i.e. Hoe 704, potent competitive inhibitor in vitro but weak in vivo | Oryza sativa | |
1.1.1.86 | additional information | no inhibition by N'-(5-methyl-1,3,4-thiadiazol-2-yl)-N-cyclopropyformyl-thiourea, N'-(5-ethyl-1,3,4-thiadiazol-2-yl)-N-cyclopropyformyl-thiourea, and N'-(5-propyl-1,3,4-thiadiazol-2-yl)-N-cyclopropyformyl-thiourea; structure-activity relationship | Oryza sativa | |
1.1.1.86 | N'-(5-(2-chlorophenyl)-1,3,4-thiadiazol-2-yl)-N-cyclopropyformyl-thiourea | - |
Oryza sativa | |
1.1.1.86 | N'-(5-(2-fluorophenyl)-1,3,4-thiadiazol-2-yl)-N-cyclopropyformyl-thiourea | - |
Oryza sativa | |
1.1.1.86 | N'-(5-(2-methyl-phenyl)-1,3,4-thiadiazol-2-yl)-N-cyclopropyformyl-thiourea | - |
Oryza sativa | |
1.1.1.86 | N'-(5-(3-methyl-phenyl)-1,3,4-thiadiazol-2-yl)-N-cyclopropyformyl-thiourea | - |
Oryza sativa | |
1.1.1.86 | N'-(5-(3-pyridinyl)-1,3,4-thiadiazol-2-yl)-N-cyclopropyformyl-thiourea | - |
Oryza sativa | |
1.1.1.86 | N'-(5-(4-chlorophenyl)-1,3,4-thiadiazol-2-yl)-N-cyclopropyformyl-thiourea | - |
Oryza sativa | |
1.1.1.86 | N'-(5-(4-methoxyphenyl)-1,3,4-thiadiazol-2-yl)-N-cyclopropyformyl-thiourea | - |
Oryza sativa | |
1.1.1.86 | N'-(5-(4-nitrophenyl)-1,3,4-thiadiazol-2-yl)-N-cyclopropyformyl-thiourea | - |
Oryza sativa | |
1.1.1.86 | N'-(5-butyl-1,3,4-thiadiazol-2-yl)-N-cyclopropyformyl-thiourea | - |
Oryza sativa | |
1.1.1.86 | N'-(5-furan-1,3,4-thiadiazol-2-yl)-N-cyclopropyformyl-thiourea | - |
Oryza sativa | |
1.1.1.86 | N'-(5-isopropyl-1,3,4-thiadiazol-2-yl)-N-cyclopropyformyl-thiourea | - |
Oryza sativa | |
1.1.1.86 | N'-(5-phenyl-1,3,4-thiadiazol-2-yl)-N-cyclopropyformyl-thiourea | - |
Oryza sativa | |
1.1.1.86 | N-(5-substituted-1,3,4-thiadiazol-2-yl)-N-cyclopropylformyl-thiourea | - |
Oryza sativa | |
1.1.1.86 | N-Hydroxy-N-isopropyloxamate | i.e. IpOHA, potent competitive inhibitor in vitro but weak in vivo | Oryza sativa |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.86 | Mg2+ | required for the alkyl migration reaction step | Oryza sativa | |
1.1.1.86 | additional information | the NADPH reaction step requires a divalent cation, e.g. Mg2+, Mn2+, or Co2+, but in a non-specific manner | Oryza sativa |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.86 | (R)-2,3-dihydroxy-3-methylbutanoate + NADP+ | Oryza sativa | - |
(S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH + H+ | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.86 | Oryza sativa | - |
- |
- |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.1.1.86 | (2R)-2,3-dihydroxy-3-methylbutanoate + NADP+ = (2S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH + H+ | catalytic mechanism, overview | Oryza sativa |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.86 | (R)-2,3-dihydroxy-3-methylbutanoate + NADP+ | - |
Oryza sativa | (S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH + H+ | - |
r | |
1.1.1.86 | (R)-2,3-dihydroxy-3-methylbutanoate + NADP+ | the enzyme performs a two-step reaction of an alkyl migration followed by an NADPH-dependent reduction rection | Oryza sativa | (S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH + H+ | - |
r | |
1.1.1.86 | additional information | structure-activity relationship | Oryza sativa | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.86 | KARI | - |
Oryza sativa |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.86 | 30 | - |
assay at | Oryza sativa |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.86 | 8 | - |
assay at | Oryza sativa |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.86 | NADP+ | - |
Oryza sativa | |
1.1.1.86 | NADPH | - |
Oryza sativa |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.1.1.86 | metabolism | the enzyme catalyzes the first common step in biosynthesis of branched-chain amino acids isoleucine, leucine and valine | Oryza sativa |