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Literature summary extracted from

  • Yokoyama, S.-I.; Suzuki, T.; Kawai, K.; Horitsu, H.; Takamizawa, K.
    Purification, characterization and structure analysis of NADPH-dependent D-xylose reductases from Candida tropicalis (1995), J. Ferment. Bioeng., 79, 217-223.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.431 0.009
-
NADPH pH 7.0, D-xylose reductase 3 Candida tropicalis
1.1.1.431 0.014
-
NADPH pH 7.0, D-xylose reductase 1 Candida tropicalis
1.1.1.431 0.018
-
NADPH pH 7.0, D-xylose reductase 2 Candida tropicalis
1.1.1.431 30
-
D-xylose pH 7.0, D-xylose reductase 2 Candida tropicalis
1.1.1.431 34
-
D-xylose pH 7.0, D-xylose reductase 3 Candida tropicalis
1.1.1.431 37
-
D-xylose pH 7.0, D-xylose reductase 1 Candida tropicalis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.431 58000
-
D-xylose reductase 1, D-xylose reductase 2, D-xylose reductase 3, gel filtration Candida tropicalis
1.1.1.431 364978
-
2 * 364978, D-xylose reductase 1, ion-spray mass spectrometry Candida tropicalis
1.1.1.431 365540
-
2 * 365540, D-xylose reductase 2, ion-spray mass spectrometry Candida tropicalis

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.431 Candida tropicalis
-
IF0 0618
-
1.1.1.431 Candida tropicalis IF0 0618
-
IF0 0618
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.431
-
Candida tropicalis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.431 47.8
-
D-xylose reductase 3 Candida tropicalis
1.1.1.431 56.9
-
D-xylose reductase 1 Candida tropicalis
1.1.1.431 81
-
D-xylose reductase 2 Candida tropicalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.431 D-galactose + NADPH + H+ D-xylose reductase 1: 49% of the activity with D-xylose, D-xylose reductase 2: 40% of the activity with D-xylose, D-xylose reductase 3: 33% of the activity with D-xylose Candida tropicalis ?
-
?
1.1.1.431 D-galactose + NADPH + H+ D-xylose reductase 1: 49% of the activity with D-xylose, D-xylose reductase 2: 40% of the activity with D-xylose, D-xylose reductase 3: 33% of the activity with D-xylose Candida tropicalis IF0 0618 ?
-
?
1.1.1.431 D-glucose + NADPH + H+ D-xylose reductase 1: 10% of the activity with D-xylose, D-xylose reductase 2: 11% of the activity with D-xylose, D-xylose reductase 3: 11% of the activity with D-xylose Candida tropicalis ?
-
?
1.1.1.431 D-glucose + NADPH + H+ D-xylose reductase 1: 10% of the activity with D-xylose, D-xylose reductase 2: 11% of the activity with D-xylose, D-xylose reductase 3: 11% of the activity with D-xylose Candida tropicalis IF0 0618 ?
-
?
1.1.1.431 D-xylose + NADPH + H+
-
Candida tropicalis xylitol + NADP+
-
?
1.1.1.431 D-xylose + NADPH + H+
-
Candida tropicalis IF0 0618 xylitol + NADP+
-
?
1.1.1.431 DL-glyceraldehyde + NADPH + H+ D-xylose reductase 1: 200% of the activity with D-xylose, D-xylose reductase 2: 268% of the activity with D-xylose, D-xylose reductase 3: 143% of the activity with D-xylose Candida tropicalis glycerol + NADP+
-
?
1.1.1.431 DL-glyceraldehyde + NADPH + H+ D-xylose reductase 1: 200% of the activity with D-xylose, D-xylose reductase 2: 268% of the activity with D-xylose, D-xylose reductase 3: 143% of the activity with D-xylose Candida tropicalis IF0 0618 glycerol + NADP+
-
?
1.1.1.431 L-arabinose + NADPH + H+ D-xylose reductase 1: 117% of the activity with D-xylose, D-xylose reductase 2: 120% of the activity with D-xylose, D-xylose reductase 3: 101% of the activity with D-xylose Candida tropicalis L-arabinitol + NADP+
-
?
1.1.1.431 L-arabinose + NADPH + H+ D-xylose reductase 1: 117% of the activity with D-xylose, D-xylose reductase 2: 120% of the activity with D-xylose, D-xylose reductase 3: 101% of the activity with D-xylose Candida tropicalis IF0 0618 L-arabinitol + NADP+
-
?

Subunits

EC Number Subunits Comment Organism
1.1.1.431 dimer 2 * 364978, D-xylose reductase 1, ion-spray mass spectrometry Candida tropicalis
1.1.1.431 dimer 2 * 365540, D-xylose reductase 2, ion-spray mass spectrometry Candida tropicalis

Synonyms

EC Number Synonyms Comment Organism
1.1.1.431 D-xylose reductase 1
-
Candida tropicalis
1.1.1.431 D-xylose reductase 2
-
Candida tropicalis
1.1.1.431 D-xylose reductase 3
-
Candida tropicalis
1.1.1.431 XR1
-
Candida tropicalis
1.1.1.431 XR2
-
Candida tropicalis
1.1.1.431 XR3
-
Candida tropicalis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.431 60
-
1 h, D-xylose reductase 1, D-xylose reductase 2, complete loss of activity Candida tropicalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.431 6
-
D-xylose reductase 1, D-xylose reductase 2 Candida tropicalis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.431 NADPH no activity with NADH Candida tropicalis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.1.1.431 Candida tropicalis D-xylose reductase 2, isoelectric focusing, pH-range: 2.5-5.0
-
4.1
1.1.1.431 Candida tropicalis D-xylose reductase 1, isoelectric focusing, pH-range: 2.5-5.0
-
4.15