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Literature summary extracted from

  • Kurakata, Y.; Uechi, A.; Yoshida, H.; Kamitori, S.; Sakano, Y.; Nishikawa, A.; Tonozuka, T.
    Structural insights into the substrate specificity and function of Escherichia coli K12 YgjK, a glucosidase belonging to the glycoside hydrolase family 63 (2008), J. Mol. Biol., 381, 116-128.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.106 expression in Escherichia coli B834 (DE3) for selenomethionine derivates Escherichia coli
3.2.1.106 expression in Escherichia coli BL21 (DE3) Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.106 230
-
 nigerose
-
 Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.106 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.106
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.106 kojibiose pH 5.0, 35°C, 30 min Escherichia coli ?
-
 ?
3.2.1.106 Maltoheptaose pH 5.0, 35°C, 30 min Escherichia coli ?
-
 ?
3.2.1.106 Maltohexaose pH 5.0, 35°C, 30 min Escherichia coli ?
-
 ?
3.2.1.106 maltopentaose pH 5.0, 35°C, 30 min Escherichia coli ?
-
 ?
3.2.1.106 maltose pH 5.0, 35°C, 30 min Escherichia coli ?
-
 ?
3.2.1.106 Maltotetraose pH 5.0, 35°C, 30 min Escherichia coli ?
-
 ?
3.2.1.106 maltotriose pH 5.0, 35°C, 30 min Escherichia coli ?
-
 ?
3.2.1.106 nigerose pH 5.0, 35°C, 30 min Escherichia coli ?
-
 ?
3.2.1.106 trehalose pH 5.0, 35°C, 30 min Escherichia coli ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.106 YgjK
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.106 42
-
 pH 5.0 Escherichia coli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.106 45
-
 loss of activity at 50°C, pH 7.5, 30 min Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.106 5
-
 42 Escherichia coli

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.106 3.5 10 residual activity higher than 90%, 4°C, 30 min Escherichia coli