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Literature summary extracted from

  • Oksanen, E.; Blakeley, M.P.; Bonnete, F.; Dauvergne, M.T.; Dauvergne, F.; Budayova-Spano, M.
    Large crystal growth by thermal control allows combined X-ray and neutron crystallographic studies to elucidate the protonation states in Aspergillus flavus urate oxidase (2009), J. R. Soc. Interface, 6 Suppl 5, 599-610.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.7.3.3 medicine urate oxidase is used to reduce toxic urate accumulation during chemotherapy Aspergillus flavus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.7.3.3 crystals of about a few tens of micrometres in size, which is nucleated previously in crystallization batch containing 5% PEG 8000, 100 mM NaCl, 8 mg/ml uox-substrate complex and 100 mM Tris-HCl pH 8.5, are used as seeds and their size and quality are further improved using a temperature-control device, large crystals of Uox, co-crystallized with its substrates analogues 8-azaxanthine, 9-methyluric acid or the natural substrate in the presence of cyanide (0.5-2 mg/ml), and soaks with the natural substrate in the absence of cyanide, diffracting to high resolutions are obtained, in the presence of different inhibitors, the crystal form of Uox has a body-centred orthorhombic symmetry and one of the largest primitive unit-cell volumes (a: 80 A, b: 96 A, c: 106 A) Aspergillus flavus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.3.3 8-Azaxanthine substrate analogue binds in the active site of the enzyme Aspergillus flavus
1.7.3.3 9-methyluric acid substrate analogue Aspergillus flavus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.7.3.3 137000
-
 for the homotetramer, determined by neutron crystallographic analysis Aspergillus flavus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.7.3.3 urate + O2 + H2O Aspergillus flavus purine metabolism 5-hydroxyisourate + H2O2 a metastable intermediate, which is further degrades to allantoin ?

Organism

EC Number Organism UniProt Comment Textmining
1.7.3.3 Aspergillus flavus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.7.3.3 commercial preparation
-
 Aspergillus flavus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.3.3 urate + O2 + H2O purine metabolism Aspergillus flavus 5-hydroxyisourate + H2O2 a metastable intermediate, which is further degrades to allantoin ?

Subunits

EC Number Subunits Comment Organism
1.7.3.3 homotetramer determined by X-ray crystallography, formed by crystallographic symmetry operations Aspergillus flavus

Synonyms

EC Number Synonyms Comment Organism
1.7.3.3 Uox
-
 Aspergillus flavus
1.7.3.3 Urate oxidase
-
 Aspergillus flavus