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Literature summary extracted from
- Construction of flavocytochrome b2-overproducing strains of the thermotolerant methylotrophic yeast Hansenula polymorpha (Pichia angusta) (2008), Microbiology, 77, 213-218.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 1.1.2.3 | synthesis | the enzyme is potentially important for bioanalytical technologies for highly selective assays of L-lactate in biological fluids and foods | Ogataea angusta |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.1.2.3 | gene CYB2, expression in the recipient strain Hansenula polymorpha C-105 impaired in glucose repression and devoid of catalase activity, under the control of the strong Hansenula polymorpha alcohol oxidase promoter in a plasmid for multicopy integration, optimization of culture conditions for recombinant cells with increased FCb2 activity | Ogataea angusta |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.2.3 | L-lactate + ferricytochrome c | Ogataea angusta | - |
pyruvate + ferrocytochrome c + H+ | - |
? |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.2.3 | Ogataea angusta | - |
i.e. Pichia angusa, gene CYB2 | - |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.1.2.3 | cell culture | optimization of culture conditions for recombinant cells of strain C-105 expressing gene CYB2 and showing increased FCb2 activity | Ogataea angusta | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.1.2.3 | 3 | - |
cell-free extract from recombinant Hansenula polymorpha cell line expressing gene CYB2 | Ogataea angusta |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.2.3 | L-lactate + ferricytochrome c | - |
Ogataea angusta | pyruvate + ferrocytochrome c + H+ | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.1.2.3 | Fcb2 | - |
Ogataea angusta |
| 1.1.2.3 | flavocytochrome b | - |
Ogataea angusta |
| 1.1.2.3 | L-lactate cytochrome c oxidoreductase | - |
Ogataea angusta |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.2.3 | 30 | - |
assay at | Ogataea angusta |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.2.3 | additional information | - |
thermostable enzyme | Ogataea angusta |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.2.3 | 8 | - |
assay at | Ogataea angusta |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.2.3 | cytochrome c | - |
Ogataea angusta | |
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Release 2023.1 (January 2023)
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