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Literature summary extracted from

  • Latypova, E.; Yang, S.; Wang, Y.S.; Wang, T.; Chavkin, T.A.; Hackett, M.; Schaefer, H.; Kalyuzhnaya, M.G.
    Genetics of the glutamate-mediated methylamine utilization pathway in the facultative methylotrophic beta-proteobacterium Methyloversatilis universalis FAM5 (2010), Mol. Microbiol., 75, 426-439.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.99.5 genes mgdA, mgdB, and mgdC, encoding subunits A, B, and large subunit c, the enzyme is encoded in a gene cluster essential for operation of the N-methylglutamate pathway, genetic structure Methyloversatilis universalis
6.3.4.12 gene gms, gsIII-like gene, DNA and amino acid sequence determination and analysis, genetic structure Methyloversatilis universalis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.4.12 Mg2+ dependent on, cannot be substituted by Mn2+ Methyloversatilis universalis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.4.12 48820
-
 x * 48820, sequence calcualtion Methyloversatilis universalis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.99.5 N-methyl-L-glutamate + acceptor + H2O Methyloversatilis universalis
-
 L-glutamate + formaldehyde + reduced acceptor
-
 ?
1.5.99.5 N-methyl-L-glutamate + acceptor + H2O Methyloversatilis universalis FAM5
-
 L-glutamate + formaldehyde + reduced acceptor
-
 ?
2.1.1.21 methylamine + L-glutamate Methyloversatilis universalis
-
 NH3 + N-methyl-L-glutamate
-
 ?
2.1.1.21 methylamine + L-glutamate Methyloversatilis universalis FAM5
-
 NH3 + N-methyl-L-glutamate
-
 ?
6.3.4.12 L-glutamic acid + ethylamine Methyloversatilis universalis
-
 theanine i.e. gamma-glutamylethylamide ?
6.3.4.12 L-glutamic acid + ethylamine Methyloversatilis universalis FAM5
-
 theanine i.e. gamma-glutamylethylamide ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.99.5 Methyloversatilis universalis
-
 genes mgdA, mgdB, and mgdC, encoding subunits A, B, and large subunit c
-
1.5.99.5 Methyloversatilis universalis FAM5
-
 genes mgdA, mgdB, and mgdC, encoding subunits A, B, and large subunit c
-
2.1.1.21 Methyloversatilis universalis
-
 FAM5
-
2.1.1.21 Methyloversatilis universalis FAM5
-
 FAM5
-
6.3.4.12 Methyloversatilis universalis D7R618 gene gms
-
6.3.4.12 Methyloversatilis universalis FAM5 D7R618 gene gms
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.21 cell extracts are prepared Methyloversatilis universalis
6.3.4.12 partially by ammonium sulfate fractionation Methyloversatilis universalis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.21 0.0113
-
 mutant strain NMGP5, protein extract from cells grown on methanol and induced with methylamine Methyloversatilis universalis
2.1.1.21 0.0118
-
 mutant strain NMGP4, protein extract from cells grown on methanol and induced with methylamine Methyloversatilis universalis
2.1.1.21 0.0223
-
 wild-type strain, protein extract from cells grown on methanol Methyloversatilis universalis
2.1.1.21 0.0557
-
 mutant strain NMGP3, protein extract from cells grown on methanol and induced with methylamine Methyloversatilis universalis
2.1.1.21 0.0705
-
 wild-type strain, protein extract from cells grown on methanol and induced with methylamine Methyloversatilis universalis
2.1.1.21 0.1113
-
 wild-type strain, protein extract from cells grown on methylamine Methyloversatilis universalis
6.3.4.12 0.022 0.205 different wild-type and mutant strains Methyloversatilis universalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.99.5 N-methyl-L-glutamate + acceptor + H2O
-
 Methyloversatilis universalis L-glutamate + formaldehyde + reduced acceptor
-
 ?
1.5.99.5 N-methyl-L-glutamate + acceptor + H2O
-
 Methyloversatilis universalis FAM5 L-glutamate + formaldehyde + reduced acceptor
-
 ?
1.5.99.5 N-methyl-L-glutamate + oxidized 2,6-dichlorophenolindophenol + H2O
-
 Methyloversatilis universalis L-glutamate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
 ?
1.5.99.5 N-methyl-L-glutamate + oxidized 2,6-dichlorophenolindophenol + H2O
-
 Methyloversatilis universalis FAM5 L-glutamate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
 ?
2.1.1.21 methylamine + L-glutamate
-
 Methyloversatilis universalis NH3 + N-methyl-L-glutamate
-
 ?
2.1.1.21 methylamine + L-glutamate
-
 Methyloversatilis universalis FAM5 NH3 + N-methyl-L-glutamate
-
 ?
6.3.4.12 L-glutamic acid + ethylamine
-
 Methyloversatilis universalis theanine i.e. gamma-glutamylethylamide ?
6.3.4.12 L-glutamic acid + ethylamine
-
 Methyloversatilis universalis FAM5 theanine i.e. gamma-glutamylethylamide ?

Subunits

EC Number Subunits Comment Organism
1.5.99.5 ? subunits A, B, and large subunit c Methyloversatilis universalis
6.3.4.12 ? x * 48820, sequence calcualtion Methyloversatilis universalis

Synonyms

EC Number Synonyms Comment Organism
1.5.99.5 N-methyl-L-glutamate:oxidoreductase
-
 Methyloversatilis universalis
1.5.99.5 N-methylglutamate dehydrogenase
-
 Methyloversatilis universalis
1.5.99.5 NMGDH
-
 Methyloversatilis universalis
2.1.1.21 N-methylglutamate synthase
-
 Methyloversatilis universalis
2.1.1.21 N-methylglutamate synthetase
-
 Methyloversatilis universalis
2.1.1.21 NMGS
-
 Methyloversatilis universalis
6.3.4.12 gamma-Glutamylmethylamide synthetase
-
 Methyloversatilis universalis
6.3.4.12 GMAS
-
 Methyloversatilis universalis

Expression

EC Number Organism Comment Expression
6.3.4.12 Methyloversatilis universalis gamma-glutamylmethylamide synthetase is induced during growth on methylated amines up

General Information

EC Number General Information Comment Organism
1.5.99.5 metabolism SoxBDAG, encoding sarcosine dehydrogenase,may be involved in oxidation of N-methyl-L-glutamate, overview Methyloversatilis universalis
1.5.99.5 additional information the enzyme is encoded in a gene cluster essential for operation of the N-methylglutamate pathway in the methylotrophic beta-proteobacterium Methyloversatilis universalis FAM5. The enzyme contains an aminomethyltransferase folate-binding domain near the C-terminus of its large subunit Methyloversatilis universalis
6.3.4.12 metabolism GMAS is involved in the gamma-glutamylmethylamine methylamine utilization pathway Methyloversatilis universalis