Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Morsy, F.; Nakajima, M.; Yoshida, T.; Fujiwara, T.; Sakamoto, T.; Wada, K.
    Subcellular localization of ferredoxin-NADP+ oxidoreductase in phycobilisome retaining oxygenic photosysnthetic organisms (2008), Photosynth. Res., 95, 73-85.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.18.1.2 NaCl quinone-dependent activity decreases at concentration below 0.1 M and over 0.5 M Arthrospira platensis
1.18.1.2 NaCl quinone-dependent activity decreases at concentrations below 0.1 M and over 0.7 M Cyanidium caldarium

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.18.1.2 NaCl ferredoxin-dependent activity decreases with increasing NaCl concentration and disappears at concentrations over 0.5 M NaCl Arthrospira platensis
1.18.1.2 NaCl ferredoxin-dependent activity decreases with increasing NaCl concentration and disappears at concentrations over 0.5 M NaCl Cyanidium caldarium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.18.1.2 0.0011
-
 NADPH in a quinone-dependent cytochrome c reduction assay Arthrospira platensis
1.18.1.2 0.0011
-
 NADPH in a quinone-dependent cytochrome c reduction assay Cyanidium caldarium
1.18.1.2 0.004
-
 NADPH in a ferredoxin-dependent cytochrome c reduction assay Arthrospira platensis
1.18.1.2 0.004
-
 NADPH in a ferredoxin-dependent cytochrome c reduction assay Cyanidium caldarium
1.18.1.2 0.011
-
 NADPH in a 2,6-dichlorophenolindophenol reduction assay Arthrospira platensis
1.18.1.2 0.02
-
 NADPH in a 2,6-dichlorophenolindophenol reduction assay Cyanidium caldarium

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.18.1.2 phycobilisome
-
 Arthrospira platensis 30089
-
1.18.1.2 thylakoid membrane
-
 Arthrospira platensis 42651
-
1.18.1.2 thylakoid membrane
-
 Cyanidium caldarium 42651
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.18.1.2 33000
-
 SDS-PAGE Cyanidium caldarium
1.18.1.2 34000
-
 gel filtration Cyanidium caldarium
1.18.1.2 46000
-
 SDS-PAGE Arthrospira platensis
1.18.1.2 47000
-
 gel filtration Arthrospira platensis
1.18.1.2 90000
-
 NaCl solubilized FNR from Spirulina platensis thylakoid membranes contained the 46000 Da FNR and a 17000 Da protein Arthrospira platensis

Organism

EC Number Organism UniProt Comment Textmining
1.18.1.2 Arthrospira platensis
-
 cyanobacterium
-
1.18.1.2 Arthrospira platensis IAM M-135
-
 cyanobacterium
-
1.18.1.2 Cyanidium caldarium A0ZSY5 red alga
-
1.18.1.2 Cyanidium caldarium RK-1 A0ZSY5 red alga
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.18.1.2
-
 Arthrospira platensis
1.18.1.2
-
 Cyanidium caldarium

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.18.1.2 25
-
 ferredoxin-dependent activity at pH 9.5 Cyanidium caldarium
1.18.1.2 30
-
 ferredoxin-dependent activity at pH 10 Arthrospira platensis
1.18.1.2 60
-
 quinone-dependent activity at pH 7.5 Arthrospira platensis
1.18.1.2 60
-
 quinone-dependent activity at pH 7.5 Cyanidium caldarium
1.18.1.2 70
-
 ferredoxin-dependent activity at pH 6.5 Arthrospira platensis
1.18.1.2 77
-
-
 Cyanidium caldarium
1.18.1.2 85
-
-
 Arthrospira platensis
1.18.1.2 95
-
 ferredoxin-dependent activity at pH 7 Arthrospira platensis
1.18.1.2 110
-
 ferredoxin-dependent activity at pH 6.5 Cyanidium caldarium
1.18.1.2 140
-
 ferredoxin-dependent activity at pH 7 Cyanidium caldarium
1.18.1.2 260
-
 quinone-dependent activity at pH 10 Arthrospira platensis
1.18.1.2 280
-
 quinone-dependent activity at pH 9.7 Arthrospira platensis
1.18.1.2 325
-
 quinone-dependent activity at pH 10 Cyanidium caldarium
1.18.1.2 400
-
 quinone-dependent activity at pH 9.7 Cyanidium caldarium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.18.1.2 NADPH + H+ + oxidized 2,6-dichlorophenolindophenol
-
 Arthrospira platensis NADP+ + reduced 2,6-dichlorophenolindophenol
-
 ?
1.18.1.2 NADPH + H+ + oxidized 2,6-dichlorophenolindophenol
-
 Cyanidium caldarium NADP+ + reduced 2,6-dichlorophenolindophenol
-
 ?
1.18.1.2 NADPH + H+ + oxidized 2,6-dichlorophenolindophenol
-
 Arthrospira platensis IAM M-135 NADP+ + reduced 2,6-dichlorophenolindophenol
-
 ?
1.18.1.2 NADPH + H+ + oxidized 2,6-dichlorophenolindophenol
-
 Cyanidium caldarium RK-1 NADP+ + reduced 2,6-dichlorophenolindophenol
-
 ?
1.18.1.2 oxidized ferredoxin + NADPH + cytochrome c
-
 Arthrospira platensis reduced ferredoxin + NADP+ + reduced cytochrome c
-
 ?
1.18.1.2 oxidized ferredoxin + NADPH + cytochrome c
-
 Cyanidium caldarium reduced ferredoxin + NADP+ + reduced cytochrome c
-
 ?
1.18.1.2 oxidized ferredoxin + NADPH + cytochrome c
-
 Arthrospira platensis IAM M-135 reduced ferredoxin + NADP+ + reduced cytochrome c
-
 ?
1.18.1.2 oxidized ferredoxin + NADPH + cytochrome c
-
 Cyanidium caldarium RK-1 reduced ferredoxin + NADP+ + reduced cytochrome c
-
 ?
1.18.1.2 phenyl-p-benzoquinone + NADPH + cytochrome c
-
 Arthrospira platensis ? + NADP+ + reduced cytochrome c
-
 ?
1.18.1.2 phenyl-p-benzoquinone + NADPH + cytochrome c
-
 Cyanidium caldarium ? + NADP+ + reduced cytochrome c
-
 ?
1.18.1.2 phenyl-p-benzoquinone + NADPH + cytochrome c
-
 Arthrospira platensis IAM M-135 ? + NADP+ + reduced cytochrome c
-
 ?
1.18.1.2 phenyl-p-benzoquinone + NADPH + cytochrome c
-
 Cyanidium caldarium RK-1 ? + NADP+ + reduced cytochrome c
-
 ?

Subunits

EC Number Subunits Comment Organism
1.18.1.2 monomer 1 * 34000, gel filtration Cyanidium caldarium
1.18.1.2 monomer 1 * 47000, gel filtration Arthrospira platensis

Synonyms

EC Number Synonyms Comment Organism
1.18.1.2 ferredoxin-NADP+ oxidoreductase
-
 Arthrospira platensis
1.18.1.2 ferredoxin-NADP+ oxidoreductase
-
 Cyanidium caldarium
1.18.1.2 FNR
-
 Arthrospira platensis
1.18.1.2 FNR
-
 Cyanidium caldarium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.18.1.2 40
-
 in a 2,6-dichlorophenolindophenol reduction assay Arthrospira platensis
1.18.1.2 40
-
 in a ferredoxin-dependent cytochrome c reduction assay Arthrospira platensis
1.18.1.2 45
-
 in a quinone-dependent cytochrome c reduction assay Arthrospira platensis
1.18.1.2 50
-
 in a ferredoxin-dependent cytochrome c reduction assay Cyanidium caldarium
1.18.1.2 55
-
 in a 2,6-dichlorophenolindophenol reduction assay Cyanidium caldarium
1.18.1.2 55
-
 in a quinone-dependent cytochrome c reduction assay Cyanidium caldarium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.18.1.2 7
-
 in a ferredoxin-dependent cytochrome c reduction assay Arthrospira platensis
1.18.1.2 7
-
 in a ferredoxin-dependent cytochrome c reduction assay Cyanidium caldarium
1.18.1.2 9.7
-
 in a 2,6-dichlorophenolindophenol reduction assay Arthrospira platensis
1.18.1.2 9.7
-
 in a 2,6-dichlorophenolindophenol reduction assay Cyanidium caldarium
1.18.1.2 9.7
-
 in a quinone-dependent cytochrome c reduction assay Arthrospira platensis
1.18.1.2 9.7
-
 in a quinone-dependent cytochrome c reduction assay Cyanidium caldarium

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.18.1.2 6.5 10 ferredoxin-dependent activity at pH 6.5 is 70 micromol/min/mg, at pH 7 is 95 micromol/min/mg and at pH 10 is 30 micromol/min/mg Arthrospira platensis
1.18.1.2 6.5 9.5 ferredoxin-dependent activity at pH 6.5 is 110 micromol/min/mg, at pH 7 is 140 micromol/min/mg and at pH 9.5is 25 micromol/min/mg Cyanidium caldarium
1.18.1.2 7.5 10 quinone-dependent activity at pH 7.5 is 60 micromol/min/mg, at pH 9.7 is 280 micromol/min/mg and at pH 10 is 260 micromol/min/mg Arthrospira platensis
1.18.1.2 7.5 10 quinone-dependent activity at pH 7.5 is 60 micromol/min/mg, at pH 9.7 is 400 micromol/min/mg and at pH 10 is 325 micromol/min/mg Cyanidium caldarium