EC Number | Cloned (Comment) | Organism |
---|---|---|
4.2.3.47 | expressed in Escherichia coli | Zea mays |
4.2.3.47 | expressed in Escherichia coli | Zea diploperennis |
4.2.3.47 | expressed in Escherichia coli | Zea perennis |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.2.3.47 | additional information | generation the two cross-convergent mutants, TPS10-B73 L356F and TPS10-dip F356L, by site-directed mutagenesis. The mutated enzymes are heterologously expressed, purified and assayed with the substrate (E,E)-farnesyl diphosphate. The mutant enzyme TPS10-B73 L356F produces less cyclic compounds than the wild type TPS10-B73 and has a product spectrum nearly identical to that of the wild type allele TPS10-dip which contains a phenylalanine at position 356. Conversely, the mutation of phenylalanine 356 to leucine in TPS10-dip results in a product profile dominated by (E)-alpha-bergamotene that is very similar to that of TPS10-B73. These results demonstrate that phenylalanine at position 356 is responsible for the decreased production of cyclic compounds in TPS10-dip and TPS10-per | Zea mays |
4.2.3.47 | additional information | generation the two cross-convergent mutants, TPS10-B73 L356F and TPS10-dip F356L, by site-directed mutagenesis. The mutated enzymes are heterologously expressed, purified and assayed with the substrate (E,E)-farnesyl diphosphate. The mutant enzyme TPS10-B73 L356F produces less cyclic compounds than the wild type TPS10-B73 and has a product spectrum nearly identical to that of the wild type allele TPS10-dip which contains a phenylalanine at position 356. Conversely, the mutation of phenylalanine 356 to leucine in TPS10-dip results in a product profile dominated by (E)-alpha-bergamotene that is very similar to that of TPS10-B73. These results demonstrate that phenylalanine at position 356 is responsible for the decreased production of cyclic compounds in TPS10-dip and TPS10-per | Zea diploperennis |
4.2.3.47 | additional information | generation the two cross-convergent mutants, TPS10-B73 L356F and TPS10-dip F356L, by site-directed mutagenesis. The mutated enzymes are heterologously expressed, purified and assayed with the substrate (E,E)-farnesyl diphosphate. The mutant enzyme TPS10-B73 L356F produces less cyclic compounds than the wild type TPS10-B73 and has a product spectrum nearly identical to that of the wild type allele TPS10-dip which contains a phenylalanine at position 356. Conversely, the mutation of phenylalanine 356 to leucine in TPS10-dip results in a product profile dominated by (E)-alpha-bergamotene that is very similar to that of TPS10-B73. These results demonstrate that phenylalanine at position 356 is responsible for the decreased production of cyclic compounds in TPS10-dip and TPS10-per | Zea perennis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.3.47 | (2E,6E)-farnesyl diphosphate | Zea mays | maize plants attacked by lepidopteran larvae emit a volatile mixture that consists mostly of the sesquiterpene olefins, (E)-alpha-bergamotene and (E)-beta-farnesene. These volatiles are produced by the herbivore-induced terpene synthase TPS10 and attract natural enemies to the damaged plants. The TPS10 products (E)-alpha-bergamotene and (E)-beta-farnesene are consistently induced by herbivory, indicating that release of TPS10 volatiles is a defense trait conserved among maize and its wild relatives | (E)-beta-farnesene + diphosphate | - |
? | |
4.2.3.47 | (2E,6E)-farnesyl diphosphate | Zea diploperennis | maize plants attacked by lepidopteran larvae emit a volatile mixture that consists mostly of the sesquiterpene olefins, (E)-alpha-bergamotene and (E)-beta-farnesene. These volatiles are produced by the herbivore-induced terpene synthase TPS10 and attract natural enemies to the damaged plants. The TPS10 products (E)-alpha-bergamotene and (E)-beta-farnesene are consistently induced by herbivory, indicating that release of TPS10 volatiles is a defense trait conserved among maize and its wild relatives | (E)-beta-farnesene + diphosphate | - |
? | |
4.2.3.47 | (2E,6E)-farnesyl diphosphate | Zea perennis | maize plants attacked by lepidopteran larvae emit a volatile mixture that consists mostly of the sesquiterpene olefins, (E)-alpha-bergamotene and (E)-beta-farnesene. These volatiles are produced by the herbivore-induced terpene synthase TPS10 and attract natural enemies to the damaged plants. The TPS10 products (E)-alpha-bergamotene and (E)-beta-farnesene are consistently induced by herbivory, indicating that release of TPS10 volatiles is a defense trait conserved among maize and its wild relatives | (E)-beta-farnesene + diphosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.3.47 | Zea diploperennis | C7E5V9 | - |
- |
4.2.3.47 | Zea mays | C7E5V7 | subsp. mexicana | - |
4.2.3.47 | Zea mays | C7E5V8 | subsp. huehuetenangensis | - |
4.2.3.47 | Zea mays | Q2NM15 | - |
- |
4.2.3.47 | Zea perennis | C7E5W0 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.2.3.47 | - |
Zea mays |
4.2.3.47 | - |
Zea diploperennis |
4.2.3.47 | - |
Zea perennis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.3.47 | (2E,6E)-farnesyl diphosphate | - |
Zea mays | (E)-beta-farnesene + diphosphate | - |
? | |
4.2.3.47 | (2E,6E)-farnesyl diphosphate | - |
Zea diploperennis | (E)-beta-farnesene + diphosphate | - |
? | |
4.2.3.47 | (2E,6E)-farnesyl diphosphate | - |
Zea perennis | (E)-beta-farnesene + diphosphate | - |
? | |
4.2.3.47 | (2E,6E)-farnesyl diphosphate | maize plants attacked by lepidopteran larvae emit a volatile mixture that consists mostly of the sesquiterpene olefins, (E)-alpha-bergamotene and (E)-beta-farnesene. These volatiles are produced by the herbivore-induced terpene synthase TPS10 and attract natural enemies to the damaged plants. The TPS10 products (E)-alpha-bergamotene and (E)-beta-farnesene are consistently induced by herbivory, indicating that release of TPS10 volatiles is a defense trait conserved among maize and its wild relatives | Zea mays | (E)-beta-farnesene + diphosphate | - |
? | |
4.2.3.47 | (2E,6E)-farnesyl diphosphate | maize plants attacked by lepidopteran larvae emit a volatile mixture that consists mostly of the sesquiterpene olefins, (E)-alpha-bergamotene and (E)-beta-farnesene. These volatiles are produced by the herbivore-induced terpene synthase TPS10 and attract natural enemies to the damaged plants. The TPS10 products (E)-alpha-bergamotene and (E)-beta-farnesene are consistently induced by herbivory, indicating that release of TPS10 volatiles is a defense trait conserved among maize and its wild relatives | Zea diploperennis | (E)-beta-farnesene + diphosphate | - |
? | |
4.2.3.47 | (2E,6E)-farnesyl diphosphate | maize plants attacked by lepidopteran larvae emit a volatile mixture that consists mostly of the sesquiterpene olefins, (E)-alpha-bergamotene and (E)-beta-farnesene. These volatiles are produced by the herbivore-induced terpene synthase TPS10 and attract natural enemies to the damaged plants. The TPS10 products (E)-alpha-bergamotene and (E)-beta-farnesene are consistently induced by herbivory, indicating that release of TPS10 volatiles is a defense trait conserved among maize and its wild relatives | Zea perennis | (E)-beta-farnesene + diphosphate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.2.3.47 | terpene synthase 10 | - |
Zea mays |
4.2.3.47 | terpene synthase 10 | - |
Zea diploperennis |
4.2.3.47 | terpene synthase 10 | - |
Zea perennis |
4.2.3.47 | terpene synthase 10-B73 | - |
Zea mays |
4.2.3.47 | terpene synthase 10-per | - |
Zea perennis |
4.2.3.47 | TPS10 | - |
Zea mays |
4.2.3.47 | TPS10 | - |
Zea diploperennis |
4.2.3.47 | TPS10 | - |
Zea perennis |
4.2.3.47 | TPS10-dip | - |
Zea diploperennis |
EC Number | Organism | Comment | Expression |
---|---|---|---|
4.2.3.47 | Zea mays | induced by herbivory | up |
4.2.3.47 | Zea diploperennis | induced by herbivory | up |
4.2.3.47 | Zea perennis | induced by herbivory | up |
EC Number | General Information | Comment | Organism |
---|---|---|---|
4.2.3.47 | physiological function | maize plants attacked by lepidopteran larvae emit a volatile mixture that consists mostly of the sesquiterpene olefins, (E)-alpha-bergamotene and (E)-beta-farnesene. These volatiles are produced by the herbivore-induced terpene synthase TPS10 and attract natural enemies to the damaged plants. The TPS10 products (E)-alpha-bergamotene and (E)-beta-farnesene are consistently induced by herbivory, indicating that release of TPS10 volatiles is a defense trait conserved among maize and its wild relatives | Zea mays |
4.2.3.47 | physiological function | maize plants attacked by lepidopteran larvae emit a volatile mixture that consists mostly of the sesquiterpene olefins, (E)-alpha-bergamotene and (E)-beta-farnesene. These volatiles are produced by the herbivore-induced terpene synthase TPS10 and attract natural enemies to the damaged plants. The TPS10 products (E)-alpha-bergamotene and (E)-beta-farnesene are consistently induced by herbivory, indicating that release of TPS10 volatiles is a defense trait conserved among maize and its wild relatives | Zea diploperennis |
4.2.3.47 | physiological function | maize plants attacked by lepidopteran larvae emit a volatile mixture that consists mostly of the sesquiterpene olefins, (E)-alpha-bergamotene and (E)-beta-farnesene. These volatiles are produced by the herbivore-induced terpene synthase TPS10 and attract natural enemies to the damaged plants. The TPS10 products (E)-alpha-bergamotene and (E)-beta-farnesene are consistently induced by herbivory, indicating that release of TPS10 volatiles is a defense trait conserved among maize and its wild relatives | Zea perennis |