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Literature summary extracted from

  • Harispe, L.; Garcia, G.; Arbildi, P.; Pascovich, L.; Chalar, C.; Zaha, A.; Fernandez, C.; Fernandez, V.
    Biochemical analysis of a recombinant glutathione transferase from the cestode Echinococcus granulosus (2010), Acta Trop., 114, 31-36.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.18 EgGST, expression of the cytosolic isozyme in Escherichia coli strain BL21(DE3) Echinococcus granulosus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.18 albendazole
-
 Echinococcus granulosus
2.5.1.18 alizarin
-
 Echinococcus granulosus
2.5.1.18 bilirubin
-
 Echinococcus granulosus
2.5.1.18 bithionol
-
 Echinococcus granulosus
2.5.1.18 bromosulfophthalein
-
 Echinococcus granulosus
2.5.1.18 cibacron
-
 Echinococcus granulosus
2.5.1.18 Cibacron blue
-
 Echinococcus granulosus
2.5.1.18 closantel
-
 Echinococcus granulosus
2.5.1.18 ellagic acid
-
 Echinococcus granulosus
2.5.1.18 febendazole
-
 Echinococcus granulosus
2.5.1.18 Hexachlorophene
-
 Echinococcus granulosus
2.5.1.18 mebendazole
-
 Echinococcus granulosus
2.5.1.18 additional information no inhibition of the cytosolic isozyme by chenodeoxycholic acid and 4-nitropyridine-N-oxide Echinococcus granulosus
2.5.1.18 Rafoxanide
-
 Echinococcus granulosus
2.5.1.18 triphenylthin chloride
-
 Echinococcus granulosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.18 additional information
-
 additional information Michaelis-Menten steady-state kinetic studies, recombinant cytosolic isozyme, overview Echinococcus granulosus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.5.1.18 cytosol cytosolic isozyme Echinococcus granulosus 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.18 25600
-
 2 * 25600, recombinant cytosolic isozyme, SDS-PAGE Echinococcus granulosus
2.5.1.18 50000
-
 recombinant cytosolic isozyme, gel filtration Echinococcus granulosus

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.18 Echinococcus granulosus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.18 recombinant cytosolic isozyme from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography Echinococcus granulosus

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.18 2.5
-
 purified recombinant cytosolic isozyme, substrate 1-chloro-2.4-dinitrobenzene Echinococcus granulosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.18 glutathione + 1-chloro-2,4-dinitrobenzene
-
 Echinococcus granulosus S-(2,4-dinitrophenyl)glutathione + HCl
-
 ?
2.5.1.18 glutathione + cumene hydroperoxide
-
 Echinococcus granulosus ?
-
 ?
2.5.1.18 glutathione + ethacrynic acid
-
 Echinococcus granulosus ?
-
 ?
2.5.1.18 glutathione + trans,trans-2,4-decadienal
-
 Echinococcus granulosus ?
-
 ?
2.5.1.18 glutathione + trans-2-nonenal
-
 Echinococcus granulosus ?
-
 ?
2.5.1.18 additional information the recombinant cytosolic GST displays glutathione peroxidase activity towards cumene hydroperoxide, and conjugates reactive carbonyls, trans-2-nonenal and trans,trans-2,4-decadienal, indicating that it may intercept damaging products of lipid peroxidation Echinococcus granulosus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.18 dimer 2 * 25600, recombinant cytosolic isozyme, SDS-PAGE Echinococcus granulosus

Synonyms

EC Number Synonyms Comment Organism
2.5.1.18 GST
-
 Echinococcus granulosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.18 25
-
 assay at Echinococcus granulosus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.5.1.18 25 55
-
 Echinococcus granulosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.18 6.5
-
 assay at Echinococcus granulosus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.18 5 8
-
 Echinococcus granulosus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.5.1.18 0.00012
-
 pH 6.5, 25°C, recombinant cytosolic isozyme Echinococcus granulosus Cibacron blue
2.5.1.18 0.0023
-
 pH 6.5, 25°C, recombinant cytosolic isozyme Echinococcus granulosus triphenylthin chloride
2.5.1.18 0.0041
-
 pH 6.5, 25°C, recombinant cytosolic isozyme Echinococcus granulosus Hexachlorophene
2.5.1.18 0.0073
-
 pH 6.5, 25°C, recombinant cytosolic isozyme Echinococcus granulosus ellagic acid
2.5.1.18 0.039
-
 pH 6.5, 25°C, recombinant cytosolic isozyme Echinococcus granulosus Rafoxanide
2.5.1.18 0.05
-
 above, pH 6.5, 25°C, recombinant cytosolic isozyme Echinococcus granulosus closantel
2.5.1.18 0.05
-
 above, pH 6.5, 25°C, recombinant cytosolic isozyme Echinococcus granulosus febendazole
2.5.1.18 0.065
-
 pH 6.5, 25°C, recombinant cytosolic isozyme Echinococcus granulosus alizarin
2.5.1.18 0.126
-
 pH 6.5, 25°C, recombinant cytosolic isozyme Echinococcus granulosus bithionol
2.5.1.18 0.16
-
 pH 6.5, 25°C, recombinant cytosolic isozyme Echinococcus granulosus albendazole
2.5.1.18 0.165
-
 pH 6.5, 25°C, recombinant cytosolic isozyme Echinococcus granulosus bilirubin
2.5.1.18 0.188
-
 pH 6.5, 25°C, recombinant cytosolic isozyme Echinococcus granulosus bromosulfophthalein
2.5.1.18 0.5
-
 above, pH 6.5, 25°C, recombinant cytosolic isozyme Echinococcus granulosus mebendazole

General Information

EC Number General Information Comment Organism
2.5.1.18 physiological function glutathione transferases are a major detoxification system in helminths Echinococcus granulosus