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Literature summary extracted from

  • Andrew Skaff, D.; Miziorko, H.M.
    A visible wavelength spectrophotometric assay suitable for high-throughput screening of 3-hydroxy-3-methylglutaryl-CoA synthase (2010), Anal. Biochem., 396, 96-102.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.3.3.10 analysis a visible wavelength spectrophotometric assay suitable for high-throughput screening of 3-hydroxy-3-methylglutaryl-CoA synthase is established Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.3.10 into the vector pET-HSyn for expression in Escherichia coli cells Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.3.10 2-benzyl-9-(3,6-dioxocyclohexa-1,4-dienyl)-2,3,7,8-tetrahydro-6H-pyrido[1,2-a]pyrazine-1,4-dione
-
 Homo sapiens
2.3.3.10 3-chloropropionyl-CoA
-
 Homo sapiens
2.3.3.10 3-hydroxy-3-methylglutaryl-CoA
-
 Homo sapiens
2.3.3.10 acetyloxy(phenyl)mercury phenyl mercuric acetate Homo sapiens
2.3.3.10 diethylcarbamothioylsulfanyl N,N-diethylcarbamodithioate disulfiram Homo sapiens
2.3.3.10 hymeglusin
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.3.10 0.073
-
 acetyl-CoA comparison of kinetic parameters measured in spectrophotometric cuvette and microplate format assays, DELTA A412 nm standard assay Homo sapiens
2.3.3.10 0.076
-
 acetyl-CoA comparison of kinetic parameters measured in spectrophotometric cuvette and microplate format assays, DELTA A300 nm standard assay Homo sapiens
2.3.3.10 0.084
-
 acetyl-CoA comparison of kinetic parameters measured in spectrophotometric cuvette and microplate format assays, DELTA A412 nm microplate assay Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.3.10 acetyl-CoA + H2O + acetoacetyl-CoA Homo sapiens
-
 (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.10 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.3.10
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.10 acetyl-CoA + H2O + acetoacetyl-CoA
-
 Homo sapiens (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
-
 ir

Synonyms

EC Number Synonyms Comment Organism
2.3.3.10 3-hydroxy-3-methylglutaryl-CoA synthase
-
 Homo sapiens
2.3.3.10 HMG-CoA
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.3.10 24
-
 activity assay, A412 nm assay Homo sapiens
2.3.3.10 30
-
 activity assay, A300 nm assay Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.3.10 8
-
 activity assay Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.3.10 CoA
-
 Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.3.10 0.0011
-
 3-hydroxy-3-methylglutaryl-CoA comparison of kinetic parameters measured in spectrophotometric cuvette and microplate format assays, DELTA A300 nm standard assay Homo sapiens
2.3.3.10 0.0015
-
 3-hydroxy-3-methylglutaryl-CoA comparison of kinetic parameters measured in spectrophotometric cuvette and microplate format assays, DELTA A412 nm standard assay Homo sapiens
2.3.3.10 0.0031
-
 3-hydroxy-3-methylglutaryl-CoA comparison of kinetic parameters measured in spectrophotometric cuvette and microplate format assays, DELTA A412 nm microplate assay Homo sapiens