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Literature summary extracted from

  • Parker, J.E.; Merkamm, M.; Manning, N.J.; Pompon, D.; Kelly, S.L.; Kelly, D.E.
    Differential azole antifungal efficacies contrasted using a Saccharomyces cerevisiae strain humanized for sterol 14 alpha-demethylase at the homologous locus (2008), Antimicrob. Agents Chemother., 52, 3597-3603.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.14.14.154 drug development the humanized yeast strain provides a useful tool for initial specificity testing for new drugs targeting CYP51 and clearly differentiates azole antifungals in a side-by-side comparison Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.154 expressed in Saccharomyces cerevisiae BY4741 under the control of the yeast CYP51 promoter Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.14.154 clotrimazole
-
Homo sapiens
1.14.14.154 clotrimazole
-
Saccharomyces cerevisiae
1.14.14.154 econazole
-
Homo sapiens
1.14.14.154 econazole
-
Saccharomyces cerevisiae
1.14.14.154 fluconazole
-
Homo sapiens
1.14.14.154 fluconazole
-
Saccharomyces cerevisiae
1.14.14.154 itraconazole
-
Homo sapiens
1.14.14.154 itraconazole
-
Saccharomyces cerevisiae
1.14.14.154 ketoconazole
-
Homo sapiens
1.14.14.154 ketoconazole
-
Saccharomyces cerevisiae
1.14.14.154 miconazole
-
Homo sapiens
1.14.14.154 miconazole
-
Saccharomyces cerevisiae
1.14.14.154 additional information humanized yeast strain as compared to the parental yeast strain shows up to 1000fold reduced susceptibility to the orally active azole drugs and reduced effectiveness for the topical drugs Homo sapiens
1.14.14.154 additional information the parental yeast strain as compared to humanized yeast strain shows up to 1000fold higher susceptibility to the orally active azole drugs Saccharomyces cerevisiae
1.14.14.154 voriconazole displays the greatest specificity Homo sapiens
1.14.14.154 voriconazole displays the greatest specificity Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.14.154 membrane
-
Homo sapiens 16020
-
1.14.14.154 microsome
-
Homo sapiens
-
-

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.154 Homo sapiens Q16850
-
-
1.14.14.154 Saccharomyces cerevisiae
-
-
-
1.14.14.154 Saccharomyces cerevisiae BY4741
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.154 lanosterol + [reduced NADPH-hemoprotein reductase] + O2
-
Saccharomyces cerevisiae ? + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
1.14.14.154 lanosterol + [reduced NADPH-hemoprotein reductase] + O2
-
Homo sapiens ? + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
1.14.14.154 lanosterol + [reduced NADPH-hemoprotein reductase] + O2
-
Saccharomyces cerevisiae BY4741 ? + [oxidized NADPH-hemoprotein reductase] + H2O
-
?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.154 CYP51
-
Saccharomyces cerevisiae
1.14.14.154 CYP51
-
Homo sapiens
1.14.14.154 Erg11p
-
Saccharomyces cerevisiae
1.14.14.154 Erg11p
-
Homo sapiens