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Literature summary extracted from

  • Zahiri, H.S.; Noghabi, K.A.; Shin, Y.C.
    Biochemical characterization of the decaprenyl diphosphate synthase of Rhodobacter sphaeroides for coenzyme Q10 production (2006), Appl. Microbiol. Biotechnol., 73, 796-806.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.5.1.91 Triton X-100 activates, 0.3% provides maximum activity Cereibacter sphaeroides
2.5.1.91 Triton X-100 the addition of Triton X-100, even at the lowest tested concentration of 0.05%, results in a significant increase in enzyme activity. Triton X-100 at 0.3% provides the maximumactivity Cereibacter sphaeroides

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.91 expression in Escherichia coli Cereibacter sphaeroides
2.5.1.91 expression in Escherichia coli. Wild-type Escherichia coli produces coenzyme Q8 and does not produce any coenzyme Q10. Recombinant Escherichia coli produces coenzyme Q10 in addition to coenzyme Q8 Cereibacter sphaeroides

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.91 0.0115
-
 (2E,6E,10E)-geranylgeranyl diphosphate pH 7.5, 37°C Cereibacter sphaeroides
2.5.1.91 0.0115
-
 geranyl geranyl diphosphate pH 7.5, 37°C Cereibacter sphaeroides
2.5.1.91 0.0131
-
 (2E,6E)-farnesyl diphosphate pH 7.5, 37°C Cereibacter sphaeroides
2.5.1.91 0.0138
-
 isopentenyl diphosphate pH 7.5, 37°C Cereibacter sphaeroides
2.5.1.91 0.0185
-
 geranyl diphosphate pH 7.5, 37°C Cereibacter sphaeroides

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.91 K+ 50% increase in activity in presence of KCl Cereibacter sphaeroides
2.5.1.91 KCl removal of KCl from the reaction mixture results in a decrease of activity Cereibacter sphaeroides
2.5.1.91 Mg2+ absolutely required for activity, maximum activity at 5 mM Cereibacter sphaeroides
2.5.1.91 MgCl2 essential for activity, highest enzyme activity with 5 mM MgCl2 Cereibacter sphaeroides

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.91 38100
-
 x * 38100, calculated from sequence Cereibacter sphaeroides
2.5.1.91 38100
-
 x * 38100, SDS-PAGE and calculated Cereibacter sphaeroides

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.91 Cereibacter sphaeroides Q5I7E1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.91
-
 Cereibacter sphaeroides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.91 (2E,6E)-farnesyl diphosphate + 7 isopentenyl diphosphate best substrate Cereibacter sphaeroides 7 diphosphate + all-trans-decaprenyl diphosphate high product specificity for decaprenyl synthesis ?
2.5.1.91 (2E,6E)-farnesyl diphosphate + 7 isopentenyl diphosphate compared with other priming substrates examined (geranyl diphosphate, geranylgeranyl diphosphate), farnesyl diphosphate results in the highest values for the reaction rate and kcat/KM. Dimethylallyl diphosphate is not a suitable substrate Cereibacter sphaeroides 7 diphosphate + all-trans-decaprenyl diphosphate decaprenyl diphosphate is the major product, other prenyl diphosphates comprise only minor components ?
2.5.1.91 (2E,6E,10E)-geranylgeranyl diphosphate + isopentenyl diphosphate
-
 Cereibacter sphaeroides diphosphate + ?
-
 ?
2.5.1.91 dimethyl allyl diphosphate + isopentenyl diphosphate
-
 Cereibacter sphaeroides diphosphate + ?
-
 ?
2.5.1.91 geranyl diphosphate + 8 isopentenyl diphosphate
-
 Cereibacter sphaeroides 8 diphosphate + all-trans-decaprenyl diphosphate decaprenyl diphosphate is the major product, other prenyl diphosphates comprise only minor components ?
2.5.1.91 geranyl diphosphate + isopentenyl diphosphate
-
 Cereibacter sphaeroides diphosphate + ?
-
 ?
2.5.1.91 geranylgeranyl diphosphate + 6 isopentenyl diphosphate
-
 Cereibacter sphaeroides 6 diphosphate + all-trans-decaprenyl diphosphate decaprenyl diphosphate is the major product, other prenyl diphosphates comprise only minor components ?
2.5.1.91 additional information catalyzes the addition of isopentenyl diphosphate to dimethyl allyl diphosphate, geranyl diphosphate, (2E,6E)-farnesyl diphosphate, and (2E,6E,10E)-geranylgeranyl diphosphate as priming substrates Cereibacter sphaeroides ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.91 ? x * 38100, calculated from sequence Cereibacter sphaeroides
2.5.1.91 ? x * 38100, SDS-PAGE and calculated Cereibacter sphaeroides

Synonyms

EC Number Synonyms Comment Organism
2.5.1.91 Ddps
-
 Cereibacter sphaeroides
2.5.1.91 Rsdds
-
 Cereibacter sphaeroides

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.91 37
-
 assay at Cereibacter sphaeroides

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.91 0.058
-
 (2E,6E,10E)-geranylgeranyl diphosphate pH 7.5, 37°C Cereibacter sphaeroides
2.5.1.91 0.06
-
 geranyl geranyl diphosphate pH 7.5, 37°C Cereibacter sphaeroides
2.5.1.91 0.067
-
 geranyl diphosphate pH 7.5, 37°C Cereibacter sphaeroides
2.5.1.91 0.14
-
 isopentenyl diphosphate pH 7.5, 37°C Cereibacter sphaeroides
2.5.1.91 0.143
-
 isopentenyl diphosphate pH 7.5, 37°C Cereibacter sphaeroides
2.5.1.91 0.147
-
 (2E,6E)-farnesyl diphosphate pH 7.5, 37°C Cereibacter sphaeroides

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.91 7.5
-
 assay at Cereibacter sphaeroides

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.5.1.91 3.62
-
 geranyl diphosphate pH 7.5, 37°C Cereibacter sphaeroides
2.5.1.91 5.04
-
 (2E,6E,10E)-geranylgeranyl diphosphate pH 7.5, 37°C Cereibacter sphaeroides
2.5.1.91 5.2
-
 geranyl geranyl diphosphate pH 7.5, 37°C Cereibacter sphaeroides
2.5.1.91 10.36
-
 isopentenyl diphosphate pH 7.5, 37°C Cereibacter sphaeroides
2.5.1.91 10.77
-
 isopentenyl diphosphate pH 7.5, 37°C Cereibacter sphaeroides
2.5.1.91 11.2
-
 (2E,6E)-farnesyl diphosphate pH 7.5, 37°C Cereibacter sphaeroides
2.5.1.91 11.22
-
 (2E,6E)-farnesyl diphosphate pH 7.5, 37°C Cereibacter sphaeroides