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Literature summary extracted from

  • Renck, D.; Ducati, R.G.; Palma, M.S.; Santos, D.S.; Basso, L.A.
    The kinetic mechanism of human uridine phosphorylase 1: Towards the development of enzyme inhibitors for cancer chemotherapy (2010), Arch. Biochem. Biophys., 497, 35-42.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.4.2.3 drug development UP12 is a target for development of enzyme inhibitors for cancer chemotherapy, design of inhibitors is intended to boost endogenous uridine levels to rescue normal tissues from the toxicity of fluoropyrimidine nucleoside chemotherapeutic agents, such as capecitabine and 5-fluorouracil. Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.3 expression of UP1 in Escherichia coli strain Rosetta (DE3) Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.3 alpha-D-ribose 1-phosphate product inhibition pattern, noncompetitive versus uridine, competitive versus phosphate, overview Homo sapiens
2.4.2.3 Uracil product inhibition pattern, noncompetitive versus uridine and phosphate, overview Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.3 additional information
-
 additional information steady-state kinetics Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.3 uridine + phosphate Homo sapiens
-
 uracil + alpha-D-ribose 1-phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.3 Homo sapiens Q16831 UP1; type 1 enzyme
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.3 recombinant UP1 3.8fold from Escherichia coli strain Rosetta (DE3) Homo sapiens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.3 7.7
-
 purified recombinant enzyme Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.3 uridine + phosphate
-
 Homo sapiens uracil + alpha-D-ribose 1-phosphate
-
 r

Synonyms

EC Number Synonyms Comment Organism
2.4.2.3 UP type 1
-
 Homo sapiens
2.4.2.3 UP1
-
 Homo sapiens
2.4.2.3 uridine phosphorylase 1
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.2.3 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.3 7.6
-
 recombinant enzyme Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.2.3 5 9 recombinant enzyme,m pH profile, overview Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.2.3 0.034
-
 alpha-D-ribose 1-phosphate pH 7.4, 37°C, versus uridine Homo sapiens
2.4.2.3 0.05
-
 Uracil pH 7.4, 37°C, versus uridine Homo sapiens
2.4.2.3 0.137
-
 Uracil pH 7.4, 37°C, versus phosphate Homo sapiens
2.4.2.3 0.494
-
 alpha-D-ribose 1-phosphate pH 7.4, 37°C, versus phosphate Homo sapiens

General Information

EC Number General Information Comment Organism
2.4.2.3 metabolism uridine phosphorylase is a key enzyme in the pyrimidine salvage pathway, catalyzing the reversible phosphorolysis of uridine to uracil and ribose-1-phosphate Homo sapiens