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Literature summary extracted from
- Catalytic mechanism of SHCHC synthase in the menaquinone biosynthesis of Escherichia coli: identification and mutational analysis of the active site residues (2009), Biochemistry, 48, 6921-6931.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.2.99.20 | expressed in Escherichia coli BL21(DE3) | Escherichia coli |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 4.2.99.20 | D128A | conserved among MenH proteins | Escherichia coli |
| 4.2.99.20 | D210A | part of the catalytically essential triad | Escherichia coli |
| 4.2.99.20 | H232A | part of the catalytically essential triad | Escherichia coli |
| 4.2.99.20 | H232K | part of the catalytically essential triad | Escherichia coli |
| 4.2.99.20 | K212A | conserved among MenH proteins | Escherichia coli |
| 4.2.99.20 | R124A | conserved among MenH proteins | Escherichia coli |
| 4.2.99.20 | R168A | conserved among MenH proteins | Escherichia coli |
| 4.2.99.20 | R90A | conserved among MenH proteins | Escherichia coli |
| 4.2.99.20 | S86A | part of the catalytically essential triad | Escherichia coli |
| 4.2.99.20 | W147A | conserved among MenH proteins | Escherichia coli |
| 4.2.99.20 | W147F | conserved among MenH proteins | Escherichia coli |
| 4.2.99.20 | Y85F | conserved among MenH proteins | Escherichia coli |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.99.20 | 0.0086 | - |
(1R,2S,5S,6S)-2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate | H232A mutant protein | Escherichia coli |  |
| 4.2.99.20 | 0.01 | - |
(1R,2S,5S,6S)-2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate | wild-type protein | Escherichia coli | |
| 4.2.99.20 | 0.014 | - |
(1R,2S,5S,6S)-2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate | Y85F mutant protein | Escherichia coli | |
| 4.2.99.20 | 0.0194 | - |
(1R,2S,5S,6S)-2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate | K212A mutant protein | Escherichia coli | |
| 4.2.99.20 | 0.0237 | - |
(1R,2S,5S,6S)-2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate | W147A mutant protein | Escherichia coli | |
| 4.2.99.20 | 0.028 | - |
(1R,2S,5S,6S)-2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate | S86A mutant protein | Escherichia coli | |
| 4.2.99.20 | 0.074 | - |
(1R,2S,5S,6S)-2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate | W147F mutant protein | Escherichia coli | |
| 4.2.99.20 | 0.084 | - |
(1R,2S,5S,6S)-2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate | R124A mutant protein | Escherichia coli | |
| 4.2.99.20 | 0.085 | - |
(1R,2S,5S,6S)-2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate | D128A mutant protein | Escherichia coli | |
| 4.2.99.20 | 0.118 | - |
(1R,2S,5S,6S)-2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate | D210A mutant protein | Escherichia coli | |
| 4.2.99.20 | 0.134 | - |
(1R,2S,5S,6S)-2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate | R168A mutant protein | Escherichia coli | |
| 4.2.99.20 | 0.159 | - |
(1R,2S,5S,6S)-2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate | R90A mutant protein | Escherichia coli | |
| 4.2.99.20 | 0.532 | - |
(1R,2S,5S,6S)-2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate | H232K mutant protein | Escherichia coli | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.99.20 | Escherichia coli | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.2.99.20 | immobilized metal ion affinity chromatography, gel filtration | Escherichia coli |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.99.20 | (1R,2S,5S,6S)-2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate | - |
Escherichia coli | (1R,6R)-2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate + pyruvate | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.2.99.20 | (1R,6R)-2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase | - |
Escherichia coli |
| 4.2.99.20 | MenH | - |
Escherichia coli |
| 4.2.99.20 | SHCHC | - |
Escherichia coli |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.99.20 | 0.00058 | - |
(1R,2S,5S,6S)-2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate | H232A mutant protein | Escherichia coli | |
| 4.2.99.20 | 0.021 | - |
(1R,2S,5S,6S)-2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate | H232K mutant protein | Escherichia coli | |
| 4.2.99.20 | 0.047 | - |
(1R,2S,5S,6S)-2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate | R90A mutant protein | Escherichia coli | |
| 4.2.99.20 | 0.056 | - |
(1R,2S,5S,6S)-2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate | W147A mutant protein | Escherichia coli | |
| 4.2.99.20 | 0.18 | - |
(1R,2S,5S,6S)-2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate | D210A mutant protein | Escherichia coli | |
| 4.2.99.20 | 0.29 | - |
(1R,2S,5S,6S)-2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate | S86A mutant protein | Escherichia coli | |
| 4.2.99.20 | 0.51 | - |
(1R,2S,5S,6S)-2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate | R124A mutant protein | Escherichia coli | |
| 4.2.99.20 | 2.58 | - |
(1R,2S,5S,6S)-2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate | Y85F mutant protein | Escherichia coli | |
| 4.2.99.20 | 2.9 | - |
(1R,2S,5S,6S)-2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate | R168A mutant protein | Escherichia coli | |
| 4.2.99.20 | 15.4 | - |
(1R,2S,5S,6S)-2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate | W147F mutant protein | Escherichia coli | |
| 4.2.99.20 | 41.5 | - |
(1R,2S,5S,6S)-2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate | K212A mutant protein | Escherichia coli | |
| 4.2.99.20 | 115 | - |
(1R,2S,5S,6S)-2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate | D128A mutant protein | Escherichia coli | |
| 4.2.99.20 | 147 | - |
(1R,2S,5S,6S)-2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate | wild-type protein | Escherichia coli | |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.99.20 | 0.0392 | - |
(1R,2S,5S,6S)-2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate | H232K mutant protein | Escherichia coli | |
| 4.2.99.20 | 0.067 | - |
(1R,2S,5S,6S)-2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate | H232A mutant protein | Escherichia coli | |
| 4.2.99.20 | 0.295 | - |
(1R,2S,5S,6S)-2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate | R90A mutant protein | Escherichia coli | |
| 4.2.99.20 | 1.5 | - |
(1R,2S,5S,6S)-2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate | D210A mutant protein | Escherichia coli | |
| 4.2.99.20 | 2.4 | - |
(1R,2S,5S,6S)-2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate | W147A mutant protein | Escherichia coli | |
| 4.2.99.20 | 6.1 | - |
(1R,2S,5S,6S)-2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate | R124A mutant protein | Escherichia coli | |
| 4.2.99.20 | 10 | - |
(1R,2S,5S,6S)-2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate | S86A mutant protein | Escherichia coli | |
| 4.2.99.20 | 15 | - |
(1R,2S,5S,6S)-2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate | R168A mutant protein | Escherichia coli | |
| 4.2.99.20 | 180 | - |
(1R,2S,5S,6S)-2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate | Y85F mutant protein | Escherichia coli | |
| 4.2.99.20 | 220 | - |
(1R,2S,5S,6S)-2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate | W147F mutant protein | Escherichia coli | |
| 4.2.99.20 | 1400 | - |
(1R,2S,5S,6S)-2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate | D128A mutant protein | Escherichia coli | |
| 4.2.99.20 | 2200 | - |
(1R,2S,5S,6S)-2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate | K212A mutant protein | Escherichia coli | |
| 4.2.99.20 | 14000 | - |
(1R,2S,5S,6S)-2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate | wild-type protein | Escherichia coli | |
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