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Literature summary extracted from

  • Han, Q.; Cai, T.; Tagle, D.A.; Robinson, H.; Li, J.
    Substrate specificity and structure of human aminoadipate aminotransferase/kynurenine aminotransferase II (2008), Biosci. Rep., 28, 205-215.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.6.1.7 KAT II complex with 2-oxoglutarate, hanging drop vapor diffusion method Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.7 3-hydroxykynurenine less than 35% inhibition at 5 mM Homo sapiens
2.6.1.7 aminoadipate less than 35% inhibition at 5 mM Homo sapiens
2.6.1.7 L-asparagine less than 35% inhibition at 5 mM Homo sapiens
2.6.1.7 L-cysteine less than 35% inhibition at 5 mM Homo sapiens
2.6.1.7 L-glutamate less than 35% inhibition at 5 mM Homo sapiens
2.6.1.7 L-histidine less than 35% inhibition at 5 mM Homo sapiens
2.6.1.7 L-lysine less than 35% inhibition at 5 mM Homo sapiens
2.6.1.7 L-phenylalanine less than 35% inhibition at 5 mM Homo sapiens
2.6.1.7 additional information not inhibited by glyoxylate Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.7 1.2
-
 2-oxoglutarate in 100 mM potassium phosphate buffer (pH 7.4), at 37°C Homo sapiens
2.6.1.7 1.4
-
 indo-3-pyruvate in 100 mM potassium phosphate buffer (pH 7.4), at 37°C Homo sapiens
2.6.1.7 1.5
-
 2-oxocaproate in 100 mM potassium phosphate buffer (pH 7.4), at 37°C Homo sapiens
2.6.1.7 1.5
-
 hydroxyphenylpyruvate in 100 mM potassium phosphate buffer (pH 7.4), at 37°C Homo sapiens
2.6.1.7 1.8
-
 phenylpyruvate in 100 mM potassium phosphate buffer (pH 7.4), at 37°C Homo sapiens
2.6.1.7 2.4
-
 2-oxo-4-methylthiobutyrate in 100 mM potassium phosphate buffer (pH 7.4), at 37°C Homo sapiens
2.6.1.7 2.8
-
 Mercaptopyruvate in 100 mM potassium phosphate buffer (pH 7.4), at 37°C Homo sapiens
2.6.1.7 3.3
-
 4-methyl-2-oxopentanoate in 100 mM potassium phosphate buffer (pH 7.4), at 37°C Homo sapiens
2.6.1.7 3.4
-
 2-oxovalerate in 100 mM potassium phosphate buffer (pH 7.4), at 37°C Homo sapiens
2.6.1.7 4.7
-
 L-kynurenine in 100 mM potassium phosphate buffer (pH 7.4), at 37°C Homo sapiens
2.6.1.7 9.7
-
 pyruvate in 100 mM potassium phosphate buffer (pH 7.4), at 37°C Homo sapiens
2.6.1.7 12.7
-
 2-oxobutyrate in 100 mM potassium phosphate buffer (pH 7.4), at 37°C Homo sapiens
2.6.1.7 12.9
-
 3-methyl-2-oxobutanoate in 100 mM potassium phosphate buffer (pH 7.4), at 37°C Homo sapiens
2.6.1.7 14.2
-
 3-methyl-2-oxobutanoate in 100 mM potassium phosphate buffer (pH 7.4), at 37°C Homo sapiens
2.6.1.7 16.8
-
 oxaloacetate in 100 mM potassium phosphate buffer (pH 7.4), at 37°C Homo sapiens
2.6.1.7 18
-
 glyoxylate in 100 mM potassium phosphate buffer (pH 7.4), at 37°C Homo sapiens
2.6.1.7 20.9
-
 2-oxoadipate in 100 mM potassium phosphate buffer (pH 7.4), at 37°C Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.6.1.39 cytoplasm
-
 Homo sapiens 5737
-

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.7 Homo sapiens Q8N5Z0
-
-
2.6.1.39 Homo sapiens Q8N5Z0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.7 DEAE-Sepharose column chromatography, hydroxyapatite column chromatography, and gel filtration Homo sapiens

Storage Stability

EC Number Storage Stability Organism
2.6.1.7 -80°C, isolated KAT II, no noticeable decrease in specific activity Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.7 L-kynurenine + 2-oxo-4-methylthiobutyrate
-
 Homo sapiens ?
-
 ?
2.6.1.7 L-kynurenine + 2-oxoadipate
-
 Homo sapiens ?
-
 ?
2.6.1.7 L-kynurenine + 2-oxobutyrate
-
 Homo sapiens kynurenic acid + 2-aminobutanoate + H2O
-
 ?
2.6.1.7 L-kynurenine + 2-oxocaproate
-
 Homo sapiens ?
-
 ?
2.6.1.7 L-kynurenine + 2-oxoglutarate
-
 Homo sapiens kynurenic acid + L-glutamate + H2O
-
 ?
2.6.1.7 L-kynurenine + 2-oxovalerate
-
 Homo sapiens ?
-
 ?
2.6.1.7 L-kynurenine + 3-methyl-2-oxobutanoate
-
 Homo sapiens ?
-
 ?
2.6.1.7 L-kynurenine + 3-methyl-2-oxopentanoate
-
 Homo sapiens ?
-
 ?
2.6.1.7 L-kynurenine + 4-methyl-2-oxopentanoate
-
 Homo sapiens ?
-
 ?
2.6.1.7 L-kynurenine + glyoxylate glyoxylate shows a relatively low affinity for KAT II Homo sapiens 4-(2-aminophenyl)-2,4-dioxobutanoate + glycine
-
 ?
2.6.1.7 L-kynurenine + hydroxyphenylpyruvate
-
 Homo sapiens ?
-
 ?
2.6.1.7 L-kynurenine + indo-3-pyruvate
-
 Homo sapiens ?
-
 ?
2.6.1.7 L-kynurenine + mercaptopyruvate
-
 Homo sapiens ?
-
 ?
2.6.1.7 L-kynurenine + oxaloacetate
-
 Homo sapiens ?
-
 ?
2.6.1.7 L-kynurenine + phenylpyruvate
-
 Homo sapiens ?
-
 ?
2.6.1.7 L-kynurenine + pyruvate
-
 Homo sapiens ?
-
 ?
2.6.1.7 additional information 2-oxoglutarate, 2-oxocaproate, phenylpyruvate, and 2-oxo-4-methylthiobutyrate are highly efficient as amino-group acceptors for KAT II Homo sapiens ?
-
 ?
2.6.1.39 L-2-aminoadipate + 2-oxoglutarate
-
 Homo sapiens 2-oxoadipate + L-glutamate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.6.1.7 KAT II
-
 Homo sapiens
2.6.1.7 kynurenine aminotransferase II
-
 Homo sapiens
2.6.1.39 AadAT
-
 Homo sapiens
2.6.1.39 aminoadipate aminotransferase
-
 Homo sapiens
2.6.1.39 aminoadipate aminotransferase/kynurenine aminotransferase II
-
 Homo sapiens
2.6.1.39 KAT II/AADAT bifunctional enzyme with kynurenine aminotransferase activity and aminoadipate aminotransferase function Homo sapiens
2.6.1.39 kynurenine aminotransferase II
-
 Homo sapiens
2.6.1.39 More KAT II is identical to AADAT (aminoadipate aminotransferase) Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.7 50
-
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.6.1.7 0.065
-
 oxaloacetate in 100 mM potassium phosphate buffer (pH 7.4), at 37°C Homo sapiens
2.6.1.7 0.36
-
 pyruvate in 100 mM potassium phosphate buffer (pH 7.4), at 37°C Homo sapiens
2.6.1.7 0.39
-
 hydroxyphenylpyruvate in 100 mM potassium phosphate buffer (pH 7.4), at 37°C Homo sapiens
2.6.1.7 0.92
-
 3-methyl-2-oxobutanoate in 100 mM potassium phosphate buffer (pH 7.4), at 37°C Homo sapiens
2.6.1.7 0.92
-
 3-methyl-2-oxopentanoate in 100 mM potassium phosphate buffer (pH 7.4), at 37°C Homo sapiens
2.6.1.7 1.49
-
 indo-3-pyruvate in 100 mM potassium phosphate buffer (pH 7.4), at 37°C Homo sapiens
2.6.1.7 2.51
-
 4-methyl-2-oxopentanoate in 100 mM potassium phosphate buffer (pH 7.4), at 37°C Homo sapiens
2.6.1.7 2.66
-
 2-oxovalerate in 100 mM potassium phosphate buffer (pH 7.4), at 37°C Homo sapiens
2.6.1.7 3.49
-
 2-oxobutyrate in 100 mM potassium phosphate buffer (pH 7.4), at 37°C Homo sapiens
2.6.1.7 3.59
-
 Mercaptopyruvate in 100 mM potassium phosphate buffer (pH 7.4), at 37°C Homo sapiens
2.6.1.7 3.64
-
 glyoxylate in 100 mM potassium phosphate buffer (pH 7.4), at 37°C Homo sapiens
2.6.1.7 4.27
-
 2-oxo-4-methylthiobutyrate in 100 mM potassium phosphate buffer (pH 7.4), at 37°C Homo sapiens
2.6.1.7 4.73
-
 phenylpyruvate in 100 mM potassium phosphate buffer (pH 7.4), at 37°C Homo sapiens
2.6.1.7 4.83
-
 2-oxocaproate in 100 mM potassium phosphate buffer (pH 7.4), at 37°C Homo sapiens
2.6.1.7 4.84
-
 2-oxoadipate in 100 mM potassium phosphate buffer (pH 7.4), at 37°C Homo sapiens
2.6.1.7 7.67
-
 2-oxoglutarate in 100 mM potassium phosphate buffer (pH 7.4), at 37°C Homo sapiens
2.6.1.7 9.76
-
 L-kynurenine in 100 mM potassium phosphate buffer (pH 7.4), at 37°C Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.6.1.7 7 9
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.7 pyridoxal 5'-phosphate
-
 Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.6.1.7 0.04
-
 pyruvate in 100 mM potassium phosphate buffer (pH 7.4), at 37°C Homo sapiens
2.6.1.7 0.065
-
 3-methyl-2-oxopentanoate in 100 mM potassium phosphate buffer (pH 7.4), at 37°C Homo sapiens
2.6.1.7 0.07
-
 3-methyl-2-oxobutanoate in 100 mM potassium phosphate buffer (pH 7.4), at 37°C Homo sapiens
2.6.1.7 0.09
-
 oxaloacetate in 100 mM potassium phosphate buffer (pH 7.4), at 37°C Homo sapiens
2.6.1.7 0.2
-
 glyoxylate in 100 mM potassium phosphate buffer (pH 7.4), at 37°C Homo sapiens
2.6.1.7 0.23
-
 2-oxoadipate in 100 mM potassium phosphate buffer (pH 7.4), at 37°C Homo sapiens
2.6.1.7 0.27
-
 2-oxobutyrate in 100 mM potassium phosphate buffer (pH 7.4), at 37°C Homo sapiens
2.6.1.7 0.27
-
 hydroxyphenylpyruvate in 100 mM potassium phosphate buffer (pH 7.4), at 37°C Homo sapiens
2.6.1.7 0.76
-
 4-methyl-2-oxopentanoate in 100 mM potassium phosphate buffer (pH 7.4), at 37°C Homo sapiens
2.6.1.7 0.78
-
 2-oxovalerate in 100 mM potassium phosphate buffer (pH 7.4), at 37°C Homo sapiens
2.6.1.7 1.08
-
 indo-3-pyruvate in 100 mM potassium phosphate buffer (pH 7.4), at 37°C Homo sapiens
2.6.1.7 1.23
-
 Mercaptopyruvate in 100 mM potassium phosphate buffer (pH 7.4), at 37°C Homo sapiens
2.6.1.7 1.75
-
 2-oxo-4-methylthiobutyrate in 100 mM potassium phosphate buffer (pH 7.4), at 37°C Homo sapiens
2.6.1.7 2.62
-
 phenylpyruvate in 100 mM potassium phosphate buffer (pH 7.4), at 37°C Homo sapiens
2.6.1.7 3.13
-
 2-oxocaproate in 100 mM potassium phosphate buffer (pH 7.4), at 37°C Homo sapiens
2.6.1.7 3.27
-
 L-kynurenine in 100 mM potassium phosphate buffer (pH 7.4), at 37°C Homo sapiens
2.6.1.7 6.24
-
 2-oxoglutarate in 100 mM potassium phosphate buffer (pH 7.4), at 37°C Homo sapiens