EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
4.2.1.30 | adenosylcobalamin | - |
Klebsiella pneumoniae | |
4.2.1.30 | ATP | - |
Klebsiella pneumoniae |
EC Number | Cloned (Comment) | Organism |
---|---|---|
4.2.1.30 | PCR products of gdh genes for co-expressing alpha-, beta- and gamma subunits of wild-type GDH or GDHA/CB and GDHAB/C fusion constructs inserted into pET32a vector using NdeI/HindIII sites. To minimize the effect of steric hindrance, a linker peptide inserted into the fusion partners by SacI sites, resulting two plasmids (pET32aGDHA/CLB and pET32aGDHALB/C) for expressing fusion enzyme GDHA/CLB and GDHALB/C, expressed in Escherichia coli AD494(DE3) | Klebsiella pneumoniae |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
4.2.1.30 | glycerol | similar to the wild-type enzyme, GDHAB/C and GDHALB/C undergo mechanism-based inactivation by glycerol during catalysis and can be reactivated by glycerol dehydratase reactivase (GDHR), adenosylcobalamin, ATP, and MgCl2 | Klebsiella pneumoniae |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
4.2.1.30 | Mg2+ | - |
Klebsiella pneumoniae |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.2.1.30 | 17700 | - |
x * 60700 + x * 229000 + x * 161000, alphabetagamma including His6-tag, SDS-PAGE. x * 83800 + x * 17700, GDHAB/C, SDS-PAGE. x * 85100 + x * 17700, GDHALB/C, SDS-PAGE. x * 60700 + x * 40900, GDHA/CB. x * 60700 + x * 42200, GDHA/CLB, SDS-PAGE | Klebsiella pneumoniae |
4.2.1.30 | 40900 | - |
x * 60700 + x * 229000 + x * 161000, alphabetagamma including His6-tag, SDS-PAGE. x * 83800 + x * 17700, GDHAB/C, SDS-PAGE. x * 85100 + x * 17700, GDHALB/C, SDS-PAGE. x * 60700 + x * 40900, GDHA/CB. x * 60700 + x * 42200, GDHA/CLB, SDS-PAGE | Klebsiella pneumoniae |
4.2.1.30 | 42200 | - |
x * 60700 + x * 229000 + x * 161000, alphabetagamma including His6-tag, SDS-PAGE. x * 83800 + x * 17700, GDHAB/C, SDS-PAGE. x * 85100 + x * 17700, GDHALB/C, SDS-PAGE. x * 60700 + x * 40900, GDHA/CB. x * 60700 + x * 42200, GDHA/CLB, SDS-PAGE | Klebsiella pneumoniae |
4.2.1.30 | 60700 | - |
x * 60700 + x * 229000 + x * 161000, alphabetagamma including His6-tag, SDS-PAGE. x * 83800 + x * 17700, GDHAB/C, SDS-PAGE. x * 85100 + x * 17700, GDHALB/C, SDS-PAGE. x * 60700 + x * 40900, GDHA/CB. x * 60700 + x * 42200, GDHA/CLB, SDS-PAGE | Klebsiella pneumoniae |
4.2.1.30 | 83800 | - |
x * 60700 + x * 229000 + x * 161000, alphabetagamma including His6-tag, SDS-PAGE. x * 83800 + x * 17700, GDHAB/C, SDS-PAGE. x * 85100 + x * 17700, GDHALB/C, SDS-PAGE. x * 60700 + x * 40900, GDHA/CB. x * 60700 + x * 42200, GDHA/CLB, SDS-PAGE | Klebsiella pneumoniae |
4.2.1.30 | 85100 | - |
x * 60700 + x * 229000 + x * 161000, alphabetagamma including His6-tag, SDS-PAGE. x * 83800 + x * 17700, GDHAB/C, SDS-PAGE. x * 85100 + x * 17700, GDHALB/C, SDS-PAGE. x * 60700 + x * 40900, GDHA/CB. x * 60700 + x * 42200, GDHA/CLB, SDS-PAGE | Klebsiella pneumoniae |
4.2.1.30 | 161000 | - |
x * 60700 + x * 229000 + x * 161000, alphabetagamma including His6-tag, SDS-PAGE. x * 83800 + x * 17700, GDHAB/C, SDS-PAGE. x * 85100 + x * 17700, GDHALB/C, SDS-PAGE. x * 60700 + x * 40900, GDHA/CB. x * 60700 + x * 42200, GDHA/CLB, SDS-PAGE | Klebsiella pneumoniae |
4.2.1.30 | 229000 | - |
x * 60700 + x * 229000 + x * 161000, alphabetagamma including His6-tag, SDS-PAGE. x * 83800 + x * 17700, GDHAB/C, SDS-PAGE. x * 85100 + x * 17700, GDHALB/C, SDS-PAGE. x * 60700 + x * 40900, GDHA/CB. x * 60700 + x * 42200, GDHA/CLB, SDS-PAGE | Klebsiella pneumoniae |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.1.30 | Klebsiella pneumoniae | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.2.1.30 | by either ion exchange chromatography or on nickel chelate column | Klebsiella pneumoniae |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.1.30 | 1,2-propanediol | - |
Klebsiella pneumoniae | propionaldehyde + H2O | - |
? | |
4.2.1.30 | glycerol | - |
Klebsiella pneumoniae | 3-hydroxypropanal + H2O | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.2.1.30 | ? | x * 60700 + x * 229000 + x * 161000, alphabetagamma including His6-tag, SDS-PAGE. x * 83800 + x * 17700, GDHAB/C, SDS-PAGE. x * 85100 + x * 17700, GDHALB/C, SDS-PAGE. x * 60700 + x * 40900, GDHA/CB. x * 60700 + x * 42200, GDHA/CLB, SDS-PAGE | Klebsiella pneumoniae |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.2.1.30 | B12-dependent glycerol dehydratase | - |
Klebsiella pneumoniae |
4.2.1.30 | GDH | - |
Klebsiella pneumoniae |
EC Number | General Information | Comment | Organism |
---|---|---|---|
4.2.1.30 | malfunction | GDH loses most of its beta-subunit after purification by either ion exchange chromatography or using a nickel chelate column | Klebsiella pneumoniae |