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Literature summary extracted from

  • Hiraishi, M.; Igarashi, K.; Kimura, S.; Wada, M.; Kitaoka, M.; Samejima, M.
    Synthesis of highly ordered cellulose II in vitro using cellodextrin phosphorylase (2009), Carbohydr. Res., 344, 2468-2473.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.49 expressed in Escherichia coli Acetivibrio thermocellus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.49 0.81
-
cellotriose
-
Acetivibrio thermocellus
2.4.1.49 0.82
-
cellotetraose
-
Acetivibrio thermocellus
2.4.1.49 69
-
cellobiose
-
Acetivibrio thermocellus

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.49 Acetivibrio thermocellus
-
-
-
2.4.1.49 Acetivibrio thermocellus YM4
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.49
-
Acetivibrio thermocellus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.49 alpha-D-glucose 1-phosphate + cellobiose
-
Acetivibrio thermocellus phosphate + cellotriose
-
?
2.4.1.49 alpha-D-glucose 1-phosphate + cellobiose
-
Acetivibrio thermocellus YM4 phosphate + cellotriose
-
?
2.4.1.49 alpha-D-glucose 1-phosphate + cellotetraose
-
Acetivibrio thermocellus phosphate + cellopentaose
-
?
2.4.1.49 alpha-D-glucose 1-phosphate + cellotetraose
-
Acetivibrio thermocellus YM4 phosphate + cellopentaose
-
?
2.4.1.49 alpha-D-glucose 1-phosphate + cellotriose
-
Acetivibrio thermocellus phosphate + cellotetraose
-
?
2.4.1.49 alpha-D-glucose 1-phosphate + cellotriose
-
Acetivibrio thermocellus YM4 phosphate + cellotetraose
-
?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.49 cellodextrin phosphorylase
-
Acetivibrio thermocellus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.49 0.12
-
cellobiose
-
Acetivibrio thermocellus
2.4.1.49 3.2
-
cellotetraose
-
Acetivibrio thermocellus
2.4.1.49 4
-
cellotriose
-
Acetivibrio thermocellus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.1.49 0.00174
-
cellobiose
-
Acetivibrio thermocellus
2.4.1.49 3.9
-
cellotetraose
-
Acetivibrio thermocellus
2.4.1.49 4.94
-
cellotriose
-
Acetivibrio thermocellus