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Literature summary extracted from

  • Nguyen, M.N.; Slominski, A.; Li, W.; Ng, Y.R.; Tuckey, R.C.
    Metabolism of vitamin d2 to 17,20,24-trihydroxyvitamin d2 by cytochrome p450scc (CYP11A1) (2009), Drug Metab. Dispos., 37, 761-767.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.15.6 17,20-dihydroxyvitamin D2 competitive inhibitor Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.15.6 0.0091
-
 cholesterol in 0.45% cyclodextrin Bos taurus
1.14.15.6 0.012
-
 20-hydroxyvitamin D2 in 0.45% cyclodextrin Bos taurus
1.14.15.6 0.0175
-
 vitamin D2 in 0.45% cyclodextrin Bos taurus
1.14.15.6 0.0181
-
 17,20-dihydroxyvitamin D2 in 0.45% cyclodextrin Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.15.6 mitochondrion
-
 Bos taurus 5739
-

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.6 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.15.6
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.15.6 adrenal cortex
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.6 17,20-dihydroxyvitamin D2 + reduced adrenodoxin + O2 is slowly metabolized Bos taurus 17,20,24-trihydroxyvitamin D2 + oxidized adrenodoxin + O2
-
 ?
1.14.15.6 20-hydroxyvitamin D2 + reduced adrenodoxin + O2 is a better substrate than vitamin D2 itself Bos taurus 17,20,24-trihydroxyvitamin D2 + oxidized adrenodoxin + O2
-
 ?
1.14.15.6 cholesterol + reduced adrenodoxin + O2
-
 Bos taurus pregnenolone + 4-methylpentanal + oxidized adrenodoxin + H2O
-
 ?
1.14.15.6 vitamin D2 + reduced adrenodoxin + O2 P450scc catalyzes three sequential hydroxylations of D2 producing 20-hydroxyvitamin D2, 17,20-dihydroxyvitamin D2, and 17,20,24-trihydroxyvitamin D2, which dissociate from the active site of P450scc and accumulate in the reaction mixture Bos taurus 17,20,24-trihydroxyvitamin D2 + oxidized adrenodoxin + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.15.6 CYP11A1
-
 Bos taurus
1.14.15.6 cytochrome P450scc
-
 Bos taurus
1.14.15.6 P450scc
-
 Bos taurus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.15.6 additional information
-
 additional information in 0.45% cyclodextrin, the kcat of cholesterol is 6.1 mol/min/mol P450scc, the kcat of vitamin D2 is 2.28 mol/min/mol P450scc, the kcat of 20-hydroxyvitamin D2 is 2.47 mol/min/mol P450scc and the kcat of 17,20-dihydroxyvitamin D2 is 0.33 mol/min/mol P450scc. In phosphatidylcholine vesicles, the kcat of cholesterol is 46 mol/min/mol P450scc, the kcat of vitamin D2 is 5.62 mol/min/mol P450scc, the kcat of 20-hydroxyvitamin D2 is 1.99 mol/min/mol P450scc and the kcat of 17,20-dihydroxyvitamin D2 is 0.21 mol/min/mol P450scc Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.15.6 adrenodoxin
-
 Bos taurus

General Information

EC Number General Information Comment Organism
1.14.15.6 metabolism vitamin D2 metabolism occurs with lower efficiency than that observed for both cholesterol and D3 metabolism by P450scc Bos taurus