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Literature summary extracted from

  • Sauerzapfe, B.; Engels, L.; Elling, L.
    Broadening the biocatalytic properties of recombinant sucrose synthase 1 from potato (Solanum tuberosum L.) by expression in Escherichia coli and Saccharomyces cerevisiae (2008), Enzyme Microb. Technol., 43, 289-296.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.13 gene sus1, expression of wild-type enzyme and mutant S11D in Escherichia coli strain BL21(DE3) reveals monosaccharides D-ribulose, D-tagatose, L-glucose, and L-rhamnose as additional acceptor substrates, expression of wild-type SuSy1 and SuSy1 S11A mutant in Saccharomyces cerevisiae strain BY4741 with phosphorylation of the wild-type enzyme at Ser11 Solanum tuberosum

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.13 S11A site-directed mutagenesis, the mutant enzyme shows an altered monosacchride accceptor substrate specificity compared to the wild-type enzyme when expressed in Saccharomyces cerevisiae Solanum tuberosum
2.4.1.13 S11D site-directed mutagenesis, the mutant enzyme shows an altered monosacchride accceptor substrate specificity compared to the wild-type enzyme when expressed in Escherichia coli Solanum tuberosum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.13 2,5-dihydroxymethyl-(3S,4R)-dihydroxypyrrolidine 30% inhibition Solanum tuberosum
2.4.1.13 5-deoxy-D-fructose 10% inhibition Solanum tuberosum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.13 0.2
-
UDP recombinant enzyme expressed in Escherichia coli Solanum tuberosum
2.4.1.13 0.3
-
UDP recombinant enzyme expressed in Saccharomyces cerevisiae Solanum tuberosum
2.4.1.13 0.6
-
UDP-alpha-D-glucose recombinant enzyme expressed in Saccharomyces cerevisiae Solanum tuberosum
2.4.1.13 1.4
-
UDP-alpha-D-glucose recombinant enzyme expressed in Escherichia coli Solanum tuberosum
2.4.1.13 5
-
D-fructose recombinant enzyme expressed in Saccharomyces cerevisiae Solanum tuberosum
2.4.1.13 5.5
-
D-fructose recombinant enzyme expressed in Escherichia coli Solanum tuberosum
2.4.1.13 60
-
sucrose recombinant enzyme expressed in Saccharomyces cerevisiae Solanum tuberosum
2.4.1.13 80.3
-
sucrose recombinant enzyme expressed in Escherichia coli Solanum tuberosum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.13 UDP-alpha-D-glucose + D-fructose Solanum tuberosum
-
UDP + sucrose
-
r

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.13 Solanum tuberosum P10691 sus1; gene sus1
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.4.1.13 phosphoprotein the enzyme is phosphorylated at Ser11 Solanum tuberosum

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.13 recombinant wild-type and mutant enzymes 450fold from Escherichia coli and 25fold from Saccharomyces cerevisiae by ion exchange and immobilized metal affinity chromatography, and gel filtration Solanum tuberosum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.13 0.01
-
crude extract, recombinant enzyme in Escherichia coli Solanum tuberosum
2.4.1.13 0.3
-
crude extract, recombinant enzyme in Saccharomyces cerevisiae Solanum tuberosum
2.4.1.13 4.5
-
purified recombinant enzyme, expressed in Escherichia coli Solanum tuberosum
2.4.1.13 6.9
-
purified recombinant enzyme, expressed in Saccharomyces cerevisiae Solanum tuberosum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.13 additional information substrate specificities of recombinant wild-type and mutant enzymes expressed in Escherichia coli or in Saccharomyces cerevisia, overview Solanum tuberosum ?
-
?
2.4.1.13 UDP-alpha-D-glucose + 1-deoxy-1-fluoro-fructose the recombinant enzyme expressed in Escherichia coli shows 175% of the activity with D-fructose, 100% for the enzyme expressed in Sacchromyces cerevisiae Solanum tuberosum ?
-
?
2.4.1.13 UDP-alpha-D-glucose + D-fructose
-
Solanum tuberosum UDP + sucrose
-
r
2.4.1.13 UDP-alpha-D-glucose + D-glucose the recombinant enzyme expressed in Sacchromyces cerevisiae shows 2% of the activity with D-fructose, no activity with the enzyme expressed in Escherichia coli Solanum tuberosum ?
-
?
2.4.1.13 UDP-alpha-D-glucose + D-lyxose the recombinant enzyme expressed in Escherichia coli shows 150% of the activity with D-fructose, 48% for the enzyme expressed in Sacchromyces cerevisiae Solanum tuberosum ?
-
?
2.4.1.13 UDP-alpha-D-glucose + D-mannose the recombinant enzyme expressed in Escherichia coli shows 75% of the activity with D-fructose, 40% for the enzyme expressed in Sacchromyces cerevisiae Solanum tuberosum ?
-
?
2.4.1.13 UDP-alpha-D-glucose + D-ribose the recombinant enzyme expressed in Sacchromyces cerevisiae shows 7% of the activity with D-fructose, no activity with the enzyme expressed in Escherichia coli Solanum tuberosum ?
-
?
2.4.1.13 UDP-alpha-D-glucose + D-ribulose the recombinant enzyme expressed in Escherichia coli shows 24% of the activity with D-fructose, no activity with enzyme expressed in Sacchromyces cerevisiae Solanum tuberosum ?
-
?
2.4.1.13 UDP-alpha-D-glucose + D-tagatose the recombinant enzyme expressed in Escherichia coli shows 43% of the activity with D-fructose, no activity with the enzyme expressed in Sacchromyces cerevisiae Solanum tuberosum ?
-
?
2.4.1.13 UDP-alpha-D-glucose + D-talose the recombinant enzyme expressed in Sacchromyces cerevisiae shows 117% of the activity with D-fructose, no activity with the enzyme expressed in Escherichia coli Solanum tuberosum ?
-
?
2.4.1.13 UDP-alpha-D-glucose + D-xylose the recombinant enzyme expressed in Escherichia coli shows 300% of the activity with D-fructose, 14% for the enzyme expressed in Sacchromyces cerevisiae Solanum tuberosum ?
-
?
2.4.1.13 UDP-alpha-D-glucose + D-xylose the recombinant enzyme expressed in Sacchromyces cerevisiae shows 42% of the activity with D-fructose, no activity with the enzyme expressed in Escherichia coli Solanum tuberosum ?
-
?
2.4.1.13 UDP-alpha-D-glucose + L-arabinose the recombinant enzyme expressed in Escherichia coli shows 490% of the activity with D-fructose, 36% for the enzyme expressed in Sacchromyces cerevisiae Solanum tuberosum ?
-
?
2.4.1.13 UDP-alpha-D-glucose + L-galactose the recombinant enzyme expressed in Sacchromyces cerevisiae shows 2% of the activity with D-fructose, no activity with the enzyme expressed in Escherichia coli Solanum tuberosum ?
-
?
2.4.1.13 UDP-alpha-D-glucose + L-glucose the recombinant enzyme expressed in Escherichia coli shows 34% of the activity with D-fructose, no activity with the enzyme expressed in Sacchromyces cerevisiae Solanum tuberosum ?
-
?
2.4.1.13 UDP-alpha-D-glucose + L-mannose the recombinant enzyme expressed in Escherichia coli shows 59% of the activity with D-fructose, 8% for the enzyme expressed in Sacchromyces cerevisiae Solanum tuberosum ?
-
?
2.4.1.13 UDP-alpha-D-glucose + L-rhamnose the recombinant enzyme expressed in Escherichia coli shows 52% of the activity with D-fructose, no activity with the enzyme expressed in Sacchromyces cerevisiae Solanum tuberosum ?
-
?
2.4.1.13 UDP-alpha-D-glucose + L-sorbose the recombinant enzyme expressed in Escherichia coli shows 96% of the activity with D-fructose, 55% for the enzyme expressed in Sacchromyces cerevisiae Solanum tuberosum ?
-
?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.13 sucrose synthase 1
-
Solanum tuberosum
2.4.1.13 SuSy1
-
Solanum tuberosum

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.4.1.13 1.5
-
-
Solanum tuberosum 2,5-dihydroxymethyl-(3S,4R)-dihydroxypyrrolidine