EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.91 | additional information | no stimulation by K+, NH4+, Na+ | Gluconobacter oxydans | |
2.5.1.91 | Triton X-100 | 0.05% significantly activates, detergent is required | Gluconobacter oxydans | |
2.5.1.91 | Triton X-100 | hardly any activity in absence of detergent. 0.05% required for full activity | Gluconobacter oxydans | |
2.5.1.91 | Tween 20 | 0.2% significantly activates, detergent is required | Gluconobacter oxydans | |
2.5.1.91 | Tween 20 | hardly any activity in absence of detergent. 0.2% required for full activity | Gluconobacter oxydans |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.5.1.91 | expression in Escherichia coli | Gluconobacter oxydans |
2.5.1.91 | expression in Escherichia coli. Expression of the ddsA gene complements the lethality resulting from a defect in the octaprenyl diphosphate synthase gene of Escherichia coli and produces coenzyme Q10, indicating that coenzyme Q10 can substitute for the function of coenzyme Q8 | Gluconobacter oxydans |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.5.1.91 | A197V | mainly heptaprenyl diphosphate is produced with smaller amounts of octaprenyl diphosphate and solanesyl diphosphate. Compared to wild-type enzyme the KM- and Vmax-values are altered | Gluconobacter oxydans |
2.5.1.91 | A197V | main products all-E-octaprenyl diphosphate and heptaprenyl diphosphate | Gluconobacter oxydans |
2.5.1.91 | A70G | a small amount of undecaprenyl diphosphate is produced with higher amounts of decaprenyl diphosphate. Compared to wild-type enzyme the KM- and Vmax-values are altered | Gluconobacter oxydans |
2.5.1.91 | A70G | main product is decaprenyl diphosphate with small amounts of undecaprenyl diphosphate | Gluconobacter oxydans |
2.5.1.91 | A70V | mainly heptaprenyl diphosphate is produced with smaller amounts of octaprenyl diphosphate and solanesyl diphosphate. Compared to wild-type enzyme the KM- and Vmax-values are altered | Gluconobacter oxydans |
2.5.1.91 | A70V | main product is all-E-heptaprenyl diphosphate | Gluconobacter oxydans |
2.5.1.91 | A70Y | complete loss of activity | Gluconobacter oxydans |
2.5.1.91 | A70Y | mutation completely abolishes the decaprenyl diphosphate synthase function, indicating that Ala70 is important for enzyme activity and the determination of the chain-length properties of DdsA | Gluconobacter oxydans |
2.5.1.91 | C63A | an even distribution in the amounts of the four products, heptaprenyl diphosphate, octaprenyl diphosphate, solanesyl diphosphate and decaprenyl diphosphate is observed. Compared to wild-type enzyme the KM- and Vmax-values are altered | Gluconobacter oxydans |
2.5.1.91 | C63A | main products all-E-octaprenyl diphosphate and all-E-solanesyl diphosphate | Gluconobacter oxydans |
2.5.1.91 | C63F | produces mostly geranylfarnesyl diphosphate and octaprenyl diphosphosphate. Compared to wild-type enzyme the KM- and Vmax-values are altered. Compared to wild-type enzyme the KM- and Vmax-values are altered | Gluconobacter oxydans |
2.5.1.91 | C63F | main products all-E-octaprenyl diphosphate and all-E-geranylfarnesyl diphosphate | Gluconobacter oxydans |
2.5.1.91 | F190A | mainly heptaprenyl diphosphate is produced with smaller amounts of octaprenyl diphosphate and solanesyl diphosphate. Compared to wild-type enzyme the KM- and Vmax-values are altered | Gluconobacter oxydans |
2.5.1.91 | F190A | main product is all-E-heptaprenyl diphosphate | Gluconobacter oxydans |
2.5.1.91 | F190W | mainly heptaprenyl diphosphate is produced with smaller amounts of octaprenyl diphosphate and solanesyl diphosphate. Compared to wild-type enzyme the KM- and Vmax-values are altered | Gluconobacter oxydans |
2.5.1.91 | F190W | main product is all-E-heptaprenyl diphosphate | Gluconobacter oxydans |
2.5.1.91 | N193A | an even distribution in the amounts of the four products, heptaprenyl diphosphate, octaprenyl diphosphate, solanesyl diphosphate and decaprenyl diphosphate is observed. Compared to wild-type enzyme the KM- and Vmax-values are altered | Gluconobacter oxydans |
2.5.1.91 | N193A | main product is decaprenyl diphosphate | Gluconobacter oxydans |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.91 | 0.00007 | - |
geranylgeranyl diphosphate | pH 7.5, 37°C, mutant enzyme A197V | Gluconobacter oxydans | |
2.5.1.91 | 0.00007 | - |
(2E,6E,10E)-geranylgeranyl diphosphate | mutant A197V, pH 7.5, 37°C | Gluconobacter oxydans | |
2.5.1.91 | 0.00009 | - |
geranylgeranyl diphosphate | pH 7.5, 37°C, mutant enzyme A70V | Gluconobacter oxydans | |
2.5.1.91 | 0.00009 | - |
(2E,6E,10E)-geranylgeranyl diphosphate | mutant A70V, pH 7.5, 37°C | Gluconobacter oxydans | |
2.5.1.91 | 0.00013 | - |
geranylgeranyl diphosphate | pH 7.5, 37°C, mutant enzyme G63F | Gluconobacter oxydans | |
2.5.1.91 | 0.00013 | - |
(2E,6E,10E)-geranylgeranyl diphosphate | mutant C63F, pH 7.5, 37°C | Gluconobacter oxydans | |
2.5.1.91 | 0.00014 | - |
(2E,6E)-farnesyl diphosphate | pH 7.5, 37°C, mutant enzyme A197V | Gluconobacter oxydans | |
2.5.1.91 | 0.00014 | - |
(2E,6E)-farnesyl diphosphate | mutant A197V, pH 7.5, 37°C | Gluconobacter oxydans | |
2.5.1.91 | 0.00016 | - |
(2E,6E)-farnesyl diphosphate | pH 7.5, 37°C, mutant enzyme G63F | Gluconobacter oxydans | |
2.5.1.91 | 0.00016 | - |
(2E,6E)-farnesyl diphosphate | mutant C63F, pH 7.5, 37°C | Gluconobacter oxydans | |
2.5.1.91 | 0.00024 | - |
geranylgeranyl diphosphate | pH 7.5, 37°C, mutant enzyme F190W | Gluconobacter oxydans | |
2.5.1.91 | 0.00024 | - |
(2E,6E,10E)-geranylgeranyl diphosphate | mutant F190W, pH 7.5, 37°C | Gluconobacter oxydans | |
2.5.1.91 | 0.00032 | - |
geranylgeranyl diphosphate | pH 7.5, 37°C | Gluconobacter oxydans | |
2.5.1.91 | 0.00032 | - |
(2E,6E,10E)-geranylgeranyl diphosphate | wild-type, pH 7.5, 37°C | Gluconobacter oxydans | |
2.5.1.91 | 0.00033 | - |
(2E,6E)-farnesyl diphosphate | pH 7.5, 37°C, mutant enzyme F190W | Gluconobacter oxydans | |
2.5.1.91 | 0.00033 | - |
(2E,6E)-farnesyl diphosphate | mutant F190W, pH 7.5, 37°C | Gluconobacter oxydans | |
2.5.1.91 | 0.00036 | - |
geranylgeranyl diphosphate | pH 7.5, 37°C, mutant enzyme F190A | Gluconobacter oxydans | |
2.5.1.91 | 0.00036 | - |
(2E,6E,10E)-geranylgeranyl diphosphate | mutant F190A, pH 7.5, 37°C | Gluconobacter oxydans | |
2.5.1.91 | 0.00038 | - |
(2E,6E)-farnesyl diphosphate | pH 7.5, 37°C, mutant enzyme F190A | Gluconobacter oxydans | |
2.5.1.91 | 0.00038 | - |
(2E,6E)-farnesyl diphosphate | mutant F190A, pH 7.5, 37°C | Gluconobacter oxydans | |
2.5.1.91 | 0.0005 | - |
(2E,6E)-farnesyl diphosphate | wild-type, pH 7.5, 37°C | Gluconobacter oxydans | |
2.5.1.91 | 0.0005 | - |
(2E,6E)-farnesyl diphosphate | pH 7.5, 37°C, wild-type enzyme | Gluconobacter oxydans | |
2.5.1.91 | 0.00063 | - |
(2E,6E)-farnesyl diphosphate | pH 7.5, 37°C, mutant enzyme G63A | Gluconobacter oxydans | |
2.5.1.91 | 0.00063 | - |
(2E,6E)-farnesyl diphosphate | mutant C63A, pH 7.5, 37°C | Gluconobacter oxydans | |
2.5.1.91 | 0.0007 | - |
(2E,6E)-farnesyl diphosphate | pH 7.5, 37°C, mutant enzyme A70V | Gluconobacter oxydans | |
2.5.1.91 | 0.0007 | - |
(2E,6E)-farnesyl diphosphate | mutant A70V, pH 7.5, 37°C | Gluconobacter oxydans | |
2.5.1.91 | 0.00072 | - |
geranylgeranyl diphosphate | pH 7.5, 37°C, mutant enzyme G63A | Gluconobacter oxydans | |
2.5.1.91 | 0.00072 | - |
(2E,6E,10E)-geranylgeranyl diphosphate | mutant C63A, pH 7.5, 37°C | Gluconobacter oxydans | |
2.5.1.91 | 0.0015 | - |
(2E,6E)-farnesyl diphosphate | pH 7.5, 37°C, mutant enzyme A70G | Gluconobacter oxydans | |
2.5.1.91 | 0.0015 | - |
(2E,6E)-farnesyl diphosphate | mutant A70G, pH 7.5, 37°C | Gluconobacter oxydans | |
2.5.1.91 | 0.0024 | - |
geranylgeranyl diphosphate | pH 7.5, 37°C, mutant enzyme A70G | Gluconobacter oxydans | |
2.5.1.91 | 0.0024 | - |
(2E,6E,10E)-geranylgeranyl diphosphate | mutant A70G, pH 7.5, 37°C | Gluconobacter oxydans | |
2.5.1.91 | 0.00275 | - |
(2E,6E)-farnesyl diphosphate | pH 7.5, 37°C, mutant enzyme N193A | Gluconobacter oxydans | |
2.5.1.91 | 0.00275 | - |
(2E,6E)-farnesyl diphosphate | mutant N193A, pH 7.5, 37°C | Gluconobacter oxydans | |
2.5.1.91 | 0.00375 | - |
geranylgeranyl diphosphate | pH 7.5, 37°C, mutant enzyme N193A | Gluconobacter oxydans | |
2.5.1.91 | 0.00375 | - |
(2E,6E,10E)-geranylgeranyl diphosphate | mutant N193A, pH 7.5, 37°C | Gluconobacter oxydans | |
2.5.1.91 | 0.007 | - |
geranyl diphosphate | pH 7.5, 37°C | Gluconobacter oxydans | |
2.5.1.91 | 0.007 | - |
geranyl diphosphate | wild-type, pH 7.5, 37°C | Gluconobacter oxydans |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.91 | Mg2+ | 10 mM, required for full activity | Gluconobacter oxydans | |
2.5.1.91 | Mg2+ | no activity below 5 mM. 10 mM required for full activity | Gluconobacter oxydans | |
2.5.1.91 | additional information | not stimulated by monovalent cations such as NH4+, Na+ and K+ | Gluconobacter oxydans | |
2.5.1.91 | additional information | no stimulation by K+, NH4+, Na+ | Gluconobacter oxydans |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.5.1.91 | 33898 | - |
x * 33898, calculated from sequence | Gluconobacter oxydans |
2.5.1.91 | 41000 | - |
x * 41000, SDS-PAGE of His-tagged recombinant protein | Gluconobacter oxydans |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.91 | (2E,6E)-farnesyl diphosphate + 7 isopentenyl diphosphate | Gluconobacter oxydans | the enzyme catalyzes the consecutive condensation of isopentenyl diphosphate with allylic diphosphates to produce decaprenyl diphosphate, which is used for the side chain of ubiquinone Q10 | 7 diphosphate + all-trans-decaprenyl diphosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.91 | Gluconobacter oxydans | O82832 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.5.1.91 | His-tagged DdsA protein is purified | Gluconobacter oxydans |
2.5.1.91 | recombinant enzyme | Gluconobacter oxydans |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.91 | (2E,6E)-farnesyl diphosphate + 7 isopentenyl diphosphate | - |
Gluconobacter oxydans | 7 diphosphate + all-trans-decaprenyl diphosphate | - |
? | |
2.5.1.91 | (2E,6E)-farnesyl diphosphate + 7 isopentenyl diphosphate | - |
Gluconobacter oxydans | 7 diphosphate + all-trans-decaprenyl diphosphate | high product specificity for decaprenyl synthesis | ? | |
2.5.1.91 | (2E,6E)-farnesyl diphosphate + 7 isopentenyl diphosphate | the enzyme catalyzes the consecutive condensation of isopentenyl diphosphate with allylic diphosphates to produce decaprenyl diphosphate, which is used for the side chain of ubiquinone Q10 | Gluconobacter oxydans | 7 diphosphate + all-trans-decaprenyl diphosphate | - |
? | |
2.5.1.91 | (2E,6E,10E)-geranylgeranyl diphosphate + isopentenyl diphosphate | - |
Gluconobacter oxydans | diphosphate + ? | - |
? | |
2.5.1.91 | geranyl diphosphate + 8 isopentenyl diphosphate | - |
Gluconobacter oxydans | 8 diphosphate + all-trans-decaprenyl diphosphate | - |
? | |
2.5.1.91 | geranyl diphosphate + isopentenyl diphosphate | - |
Gluconobacter oxydans | diphosphate + ? | - |
? | |
2.5.1.91 | geranylgeranyl diphosphate + 6 isopentenyl diphosphate | - |
Gluconobacter oxydans | 6 diphosphate + all-trans-decaprenyl diphosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.5.1.91 | ? | x * 33898, calculated from sequence | Gluconobacter oxydans |
2.5.1.91 | ? | x * 41000, SDS-PAGE of His-tagged recombinant protein | Gluconobacter oxydans |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.5.1.91 | ddsa | - |
Gluconobacter oxydans |
2.5.1.91 | DdsA protein | - |
Gluconobacter oxydans |
2.5.1.91 | decaprenyl diphosphate synthase | - |
Gluconobacter oxydans |
2.5.1.91 | decaprenyl-PP synthase | - |
Gluconobacter oxydans |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.5.1.91 | 40 | 45 | - |
Gluconobacter oxydans |
2.5.1.91 | 40 | 45 | substrate: farnesyl diphosphate | Gluconobacter oxydans |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.5.1.91 | 7.5 | 8 | - |
Gluconobacter oxydans |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.5.1.91 | physiological function | the enzyme catalyzes the consecutive condensation of isopentenyl diphosphate with allylic diphosphates to produce decaprenyl diphosphate, which is used for the side chain of ubiquinone Q10 | Gluconobacter oxydans |