Literature summary extracted from
Hughes, R.K.; Lawson, D.M.; Hornostaj, A.R.; Fairhurst, S.A.; Casey, R.
Mutagenesis and modelling of linoleate-binding to pea seed lipoxygenase (2001), Eur. J. Biochem., 268, 1030-1040.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
1.13.11.B6 |
sequence comparison and homology modelling |
Pisum sativum |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
1.13.11.B6 |
F580A |
kcat/KM for linoleate hydroperoxidation is increased by 35% |
Pisum sativum |
1.13.11.B6 |
F580A |
possesses activity profile that is similar to wild-type |
Pisum sativum |
1.13.11.B6 |
F580V |
possesses activity profile that is similar to wild-type |
Pisum sativum |
1.13.11.B6 |
K578/T579RS |
possesses activity profile that is similar to wild-type |
Pisum sativum |
1.13.11.B6 |
L569V |
kcat/KM for linoleate hydroperoxidation is reduced by 38% |
Pisum sativum |
1.13.11.B6 |
T579F |
kcat/KM for linoleate hydroperoxidation is reduced by 89% |
Pisum sativum |
1.13.11.B6 |
T579S |
possesses activity profile that is similar to wild-type |
Pisum sativum |
1.13.11.B6 |
V570I |
possesses activity profile that is similar to wild-type |
Pisum sativum |
1.13.11.B6 |
W523A |
kcat/KM for linoleate hydroperoxidation is reduced by 98% |
Pisum sativum |
KM Value [mM]
EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
---|
1.13.11.B6 |
0.9 |
- |
linoleate |
wild-type enzyme |
Pisum sativum |
|
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
1.13.11.B6 |
100000 |
- |
x * 100000, wild-type enzyme and mutant enzymes T579F, L569V and W523A, SDS-PAGE |
Pisum sativum |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
1.13.11.B6 |
Pisum sativum |
- |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
1.13.11.B6 |
- |
Pisum sativum |
Source Tissue
EC Number |
Source Tissue |
Comment |
Organism |
Textmining |
---|
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
1.13.11.B6 |
linoleate + O2 |
identification of some of the primary determinants at the catalytic centre of pea 9/13-LOX. Validation of a model of linoleate-binding to this enzyme. Evidence is provided that the primary determinants of pocket volume and conformation are more important than substrate orientation in pea lipoxygenase catalysis. Inverse models may account for the positional specificity of only selected plant lipoxygenases |
Pisum sativum |
(10E,12Z)-9-hydroperoxy-10,12-octadecadienoate + (9Z,11E)-13-hydroperoxy-9,11-octadecadienoate |
ratio of (9Z,11E)-13-hydroperoxy-9,11-octadecadienoate to (10E,12Z)-9-hydroperoxy-10,12-octadecadienoate: 1.1 (wild-type enzyme), 1.6 (mutant enzyme T579S), 1.5 (mutant enzyme V570I), 1.4 (mutant enzyme T579F), 1.3 (mutant enzyme F580A), 0.9 (mutant enzyme L569V), 1.7 (mutant enzyme W523A), 1.3 (mutant enzyme F580V) |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
1.13.11.B6 |
? |
x * 100000, wild-type enzyme and mutant enzymes T579F, L569V and W523A, SDS-PAGE |
Pisum sativum |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
1.13.11.B6 |
9/13-lipoxygenase |
- |
Pisum sativum |
1.13.11.B6 |
9/13-LOX |
- |
Pisum sativum |
Turnover Number [1/s]
EC Number |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Comment |
Organism |
Structure |
---|
1.13.11.B6 |
65 |
- |
linoleate |
wild-type enzyme |
Pisum sativum |
|
kcat/KM [mM/s]
EC Number |
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Comment |
Organism |
Structure |
---|
1.13.11.B6 |
74 |
- |
linoleate |
wild-type enzyme |
Pisum sativum |
|