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Literature summary extracted from

  • Bhavani, B.S.; Rajaram, V.; Bisht, S.; Kaul, P.; Prakash, V.; Murthy, M.R.; Appaji Rao, N.; Savithri, H.S.
    Importance of tyrosine residues of Bacillus stearothermophilus serine hydroxymethyltransferase in cofactor binding and L-allo-Thr cleavage (2008), FEBS J., 275, 4606-4619.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.2.1 expressed in Escherichia coli BL21(DE3) cells Geobacillus stearothermophilus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.2.1 crystals of Y51F and Y61A SHMT mutants are obtained by hanging drop vapour diffusion using 50% (v/v) 2-methyl 2,4-pentanediol as the precipitant Geobacillus stearothermophilus

Protein Variants

EC Number Protein Variants Comment Organism
2.1.2.1 Y51F the mutation results in a complete loss of tetrahydrofolate-dependent and tetrahydrofolate-independent activities Geobacillus stearothermophilus
2.1.2.1 Y61A the mutation results in a complete loss of tetrahydrofolate-dependent and tetrahydrofolate independent activities Geobacillus stearothermophilus

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.1 Geobacillus stearothermophilus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.2.1 ammonium sulfate precipitation and DEAE-cellulose column chromatography Geobacillus stearothermophilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.2.1 0.05
-
 mutant enzyme Y61A, using L-serine as substrate, at 37°C Geobacillus stearothermophilus
2.1.2.1 5
-
 wild type enzyme, using L-serine as substrate, at 37°C Geobacillus stearothermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.2.1 L-serine + tetrahydrofolate
-
 Geobacillus stearothermophilus glycine + 5,10-methylenetetrahydrofolate + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.2.1 serine hydroxymethyltransferase
-
 Geobacillus stearothermophilus
2.1.2.1 SHMT
-
 Geobacillus stearothermophilus

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.2.1 pyridoxal 5'-phosphate wild type enzyme contains 1 mol per mole of subunit Geobacillus stearothermophilus