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Literature summary extracted from

  • Garscha, U.; Oliw, E.H.
    Critical amino acids for the 8(R)-dioxygenase activity of linoleate diol synthase. A comparison with cyclooxygenases (2008), FEBS Lett., 582, 3547-3551.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.60 expressed in Spodoptera frugiperda (Sf21) insect cells with native 8R-dioxygenase and hydroperoxide isomerase activities Gaeumannomyces graminis
5.4.4.6 expressed in Spodoptera frugiperda (Sf21) insect cells with native 8R-dioxygenase and hydroperoxide isomerase activities Gaeumannomyces graminis

Protein Variants

EC Number Protein Variants Comment Organism
1.13.11.60 H203Q weak activity Gaeumannomyces graminis
1.13.11.60 H379Q inactive mutant enzyme Gaeumannomyces graminis
1.13.11.60 K540L inactive mutant enzyme Gaeumannomyces graminis
1.13.11.60 K540Q inactive mutant enzyme Gaeumannomyces graminis
1.13.11.60 K540R inactive mutant enzyme Gaeumannomyces graminis
1.13.11.60 Y329F weak activity Gaeumannomyces graminis
1.13.11.60 Y329L inactive mutant enzyme Gaeumannomyces graminis
1.13.11.60 Y376F inactive mutant enzyme Gaeumannomyces graminis
1.13.11.60 Y378F inactive mutant enzyme Gaeumannomyces graminis
1.13.11.60 Y378S inactive mutant enzyme Gaeumannomyces graminis
1.13.11.60 Y531F inactive mutant enzyme Gaeumannomyces graminis
5.4.4.6 H203Q weak activity Gaeumannomyces graminis
5.4.4.6 H379Q inactive mutant enzyme Gaeumannomyces graminis
5.4.4.6 K540L inactive mutant enzyme Gaeumannomyces graminis
5.4.4.6 K540Q inactive mutant enzyme Gaeumannomyces graminis
5.4.4.6 K540R inactive mutant enzyme Gaeumannomyces graminis
5.4.4.6 Y329F weak activity Gaeumannomyces graminis
5.4.4.6 Y329L inactive mutant enzyme Gaeumannomyces graminis
5.4.4.6 Y376F inactive mutant enzyme Gaeumannomyces graminis
5.4.4.6 Y378F inactive mutant enzyme Gaeumannomyces graminis
5.4.4.6 Y378S inactive mutant enzyme Gaeumannomyces graminis
5.4.4.6 Y531F inactive mutant enzyme Gaeumannomyces graminis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.4.4.6 (8R,9Z,12Z)-8-hydroperoxy-9,12-octadecadienoate Gaeumannomyces graminis
-
 (7S,8S,9Z,12Z)-7,8-dihydroxy-9,12-octadecadienoate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.60 Gaeumannomyces graminis Q9UUS2
-
-
5.4.4.6 Gaeumannomyces graminis Q9UUS2
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.60 linoleate + O2
-
 Gaeumannomyces graminis (8R,9Z,12Z)-8-hydroperoxy-9,12-octadecadienoate
-
 ?
5.4.4.6 (8R,9Z,12Z)-8-hydroperoxy-9,12-octadecadienoate
-
 Gaeumannomyces graminis (7S,8S,9Z,12Z)-7,8-dihydroxy-9,12-octadecadienoate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.11.60 7,8-LDS bifunctional enzyme Gaeumannomyces graminis
1.13.11.60 7,8-linoleate diol synthase bifunctional enzyme Gaeumannomyces graminis
1.13.11.60 8R-dioxygenase
-
 Gaeumannomyces graminis
5.4.4.6 7,8-LDS bifunctional enzyme Gaeumannomyces graminis
5.4.4.6 7,8-linoleate diol synthase bifunctional enzyme Gaeumannomyces graminis

Cofactor

EC Number Cofactor Comment Organism Structure
1.13.11.60 heme
-
 Gaeumannomyces graminis
5.4.4.6 heme
-
 Gaeumannomyces graminis