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Literature summary extracted from

  • Pohanka, M.; Zboril, P.
    Amperometric biosensor for D-lactate assay (2008), Food Technol. Biotechnol., 46, 107-110.
No PubMed abstract available

Application

EC Number Application Comment Organism
1.1.2.4 additional information carbon paste electrode-based biosensor with immobilized D-LCR seems to be optimal for analysis, representing significant advantage due to simplification of the whole device, i.e., the sensor exerts lower limit of detection under supposed concentration of D-lactate in real samples, low material demands, simplicity of D-LCR biosensor and short total time of analysis of about 2 min, pointing at the sensor possibilities in commercial applications Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
1.1.2.4 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.2.4 by affinity chromatography Saccharomyces cerevisiae

Storage Stability

EC Number Storage Stability Organism
1.1.2.4 -20°C Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.2.4 D-lactate + 2 ferricytochrome c substrate specific for D-LCR. D-LCR is efficient enough in transferring electrons from D-lactate to the next mediators Saccharomyces cerevisiae pyruvate + 2 ferrocytochrome c
-
?

Synonyms

EC Number Synonyms Comment Organism
1.1.2.4 D-lactate cytochrome c oxidoreductase
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Saccharomyces cerevisiae
1.1.2.4 D-LCR
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Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.2.4 7
-
-
Saccharomyces cerevisiae