EC Number | Cloned (Comment) | Organism |
---|---|---|
5.3.3.4 | a 75-bp and a 125-bp fragment of the mmlJ gene are cloned into the vector pGEM-T Easy | Pseudomonas reinekei |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.3.1.174 | additional information | generation of a mmlC knockout strain | Pseudomonas reinekei |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
5.3.3.4 | 10000 | - |
determined by SDS-PAGE | Pseudomonas reinekei |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.174 | 4-methyl-3-oxoadipyl-CoA + CoA | Pseudomonas reinekei | - |
methylsuccinyl-CoA + acetyl-CoA | - |
? | |
2.8.3.6 | succinyl-CoA + 3-oxoadipate | Pseudomonas reinekei | - |
succinate + 3-oxoadipyl-CoA | - |
? | |
5.3.3.4 | (S)-5-oxo-2,5-dihydrofuran-2-acetate | Pseudomonas reinekei | - |
5-oxo-4,5-dihydrofuran-2-acetate | - |
? | |
5.3.3.4 | 3-methylmuconolactone | Pseudomonas reinekei | - |
4-methyl-3-oxoadipate enol-lactone | - |
? | |
5.3.3.4 | muconolactone | Pseudomonas reinekei | - |
3-oxoadipate enol-lactone | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.174 | Pseudomonas reinekei | D2I940 | - |
- |
2.8.3.6 | Pseudomonas reinekei | - |
- |
- |
3.1.1.24 | Pseudomonas reinekei | - |
- |
- |
5.3.3.4 | Pseudomonas reinekei | C5MR75 | - |
- |
5.4.99.14 | Pseudomonas reinekei | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
5.3.3.4 | MmlJ is partially purified by anion exchange chromatography using a MonoQ HR 5/5 column | Pseudomonas reinekei |
5.4.99.14 | MonoQ column chromatography | Pseudomonas reinekei |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.174 | 4-methyl-3-oxoadipyl-CoA + CoA | - |
Pseudomonas reinekei | methylsuccinyl-CoA + acetyl-CoA | - |
? | |
2.8.3.6 | succinyl-CoA + 3-oxoadipate | - |
Pseudomonas reinekei | succinate + 3-oxoadipyl-CoA | - |
? | |
3.1.1.24 | 3-methylmuconolactone + H2O | - |
Pseudomonas reinekei | ? | - |
? | |
3.1.1.24 | 4-methyl-3-oxoadipate enol-lactone + H2O | - |
Pseudomonas reinekei | 4-methyl-3-oxoadipate + H+ | - |
? | |
5.3.3.4 | (S)-5-oxo-2,5-dihydrofuran-2-acetate | - |
Pseudomonas reinekei | 5-oxo-4,5-dihydrofuran-2-acetate | - |
? | |
5.3.3.4 | 3-methylmuconolactone | - |
Pseudomonas reinekei | 4-methyl-3-oxoadipate enol-lactone | - |
? | |
5.3.3.4 | muconolactone | - |
Pseudomonas reinekei | 3-oxoadipate enol-lactone | - |
? | |
5.4.99.14 | 3-methylmuconolactone | - |
Pseudomonas reinekei | 4-methyl-3-oxoadipate enollactone | - |
? | |
5.4.99.14 | 4-Carboxymethyl-4-methylbut-2-en-1,4-olide | - |
Pseudomonas reinekei | 4-Carboxymethyl-3-methylbut-2-en-1,4-olide | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.3.1.174 | mmlC | - |
Pseudomonas reinekei |
2.3.1.174 | mmlC | gene encoding for a 3-oxoadipyl-CoA thiolase | Pseudomonas reinekei |
2.8.3.6 | 3-oxoadipate:succinyl-coenzyme A transferase | - |
Pseudomonas reinekei |
2.8.3.6 | CatIJ | - |
Pseudomonas reinekei |
2.8.3.6 | PcaIJ | - |
Pseudomonas reinekei |
3.1.1.24 | 4-methyl-3-oxoadipate enol-lactone hydrolase | - |
Pseudomonas reinekei |
3.1.1.24 | mmlL | - |
Pseudomonas reinekei |
5.3.3.4 | Methylmuconolactone isomerase | - |
Pseudomonas reinekei |
5.3.3.4 | MmlJ | - |
Pseudomonas reinekei |
5.3.3.4 | Muconolactone isomerase | - |
Pseudomonas reinekei |
5.4.99.14 | 4-Methylmuconolactone methylisomerase | - |
Pseudomonas reinekei |
5.4.99.14 | methymuconolactone isomerase | - |
Pseudomonas reinekei |
5.4.99.14 | MmlJ | - |
Pseudomonas reinekei |
EC Number | General Information | Comment | Organism |
---|---|---|---|
5.3.3.4 | metabolism | the mml gene cluster consists of 10 genes encoding enzymes involved in the metabolism of 4-methylmuconolactone | Pseudomonas reinekei |