EC Number | Cloned (Comment) | Organism |
---|---|---|
4.2.1.121 | expression in Escherichia coli | Solanum lycopersicum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.1.121 | 0.048 | - |
(9S,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoate | pH 7.0, 25°C | Solanum lycopersicum | |
4.2.1.121 | 0.067 | - |
(9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate | pH 7.0, 25°C | Solanum lycopersicum |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.2.1.121 | 55254 | - |
x * 55254, calculated from sequence | Solanum lycopersicum |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.1.121 | Solanum lycopersicum | Q9FPM6 | LeDES is a single copy gene located on the distal half of the long arm of chromosome one; cv. Castlemart | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.2.1.121 | - |
Solanum lycopersicum |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
4.2.1.121 | root | LeDES transcripts are most abundant in roots. A low level of LeDES mRNA is observed in stem tissue, but no accumulation is detected in flower buds, petioles, cotyledons, or leaves. Extracts from roots of young plants, but not extracts from stem or leaf tissue, catalyze efficient formation of colneleate from the hydroperoxide precursor | Solanum lycopersicum | - |
4.2.1.121 | stem | LeDES transcripts are most abundant in roots. A low level of LeDES mRNA is observed in stem tissue, but no accumulation is detected in flower buds, petioles, cotyledons, or leaves. Extracts from roots of young plants, but not extracts from stem or leaf tissue, catalyze efficient formation of colneleate from the hydroperoxide precursor | Solanum lycopersicum | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.1.121 | (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate | poorly active against the the corresponding 13-hydroperoxide | Solanum lycopersicum | (8E)-9-[(1E,3Z)-nona-1,3-dien-1-yloxy]non-8-enoic acid + H2O | i.e. colneleate, characterization of the product | ? | |
4.2.1.121 | (9S,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoate | poorly active against the the corresponding 13-hydroperoxide | Solanum lycopersicum | (8E)-9-[(1E,3Z,6Z)-nona-1,3,6-trien-1-yloxy]non-8-enoic acid + H2O | i.e. colnelenate | ? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.2.1.121 | ? | x * 55254, calculated from sequence | Solanum lycopersicum |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.2.1.121 | CYP74 cytochrome P-450 | - |
Solanum lycopersicum |
4.2.1.121 | CYP74D1 | - |
Solanum lycopersicum |
4.2.1.121 | LeDES | - |
Solanum lycopersicum |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.1.121 | 500 | - |
(9S,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoate | pH 7.0, 25°C | Solanum lycopersicum | |
4.2.1.121 | 890 | - |
(9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate | pH 7.0, 25°C | Solanum lycopersicum |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
4.2.1.121 | cytochrome P-450 | the enzyme has spectral properties of cytochrome P-450 | Solanum lycopersicum |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.1.121 | 10420 | - |
(9S,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoate | pH 7.0, 25°C | Solanum lycopersicum | |
4.2.1.121 | 13280 | - |
(9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate | pH 7.0, 25°C | Solanum lycopersicum |