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Literature summary extracted from

  • Itoh, A.; Howe, G.A.
    Molecular cloning of a divinyl ether synthase. Identification as a CYP74 cytochrome P-450 (2001), J. Biol. Chem., 276, 3620-3627.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.121 expression in Escherichia coli Solanum lycopersicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.121 0.048
-
(9S,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoate pH 7.0, 25°C Solanum lycopersicum
4.2.1.121 0.067
-
(9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate pH 7.0, 25°C Solanum lycopersicum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.121 55254
-
x * 55254, calculated from sequence Solanum lycopersicum

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.121 Solanum lycopersicum Q9FPM6 LeDES is a single copy gene located on the distal half of the long arm of chromosome one; cv. Castlemart
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.121
-
Solanum lycopersicum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.1.121 root LeDES transcripts are most abundant in roots. A low level of LeDES mRNA is observed in stem tissue, but no accumulation is detected in flower buds, petioles, cotyledons, or leaves. Extracts from roots of young plants, but not extracts from stem or leaf tissue, catalyze efficient formation of colneleate from the hydroperoxide precursor Solanum lycopersicum
-
4.2.1.121 stem LeDES transcripts are most abundant in roots. A low level of LeDES mRNA is observed in stem tissue, but no accumulation is detected in flower buds, petioles, cotyledons, or leaves. Extracts from roots of young plants, but not extracts from stem or leaf tissue, catalyze efficient formation of colneleate from the hydroperoxide precursor Solanum lycopersicum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.121 (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate poorly active against the the corresponding 13-hydroperoxide Solanum lycopersicum (8E)-9-[(1E,3Z)-nona-1,3-dien-1-yloxy]non-8-enoic acid + H2O i.e. colneleate, characterization of the product ?
4.2.1.121 (9S,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoate poorly active against the the corresponding 13-hydroperoxide Solanum lycopersicum (8E)-9-[(1E,3Z,6Z)-nona-1,3,6-trien-1-yloxy]non-8-enoic acid + H2O i.e. colnelenate ?

Subunits

EC Number Subunits Comment Organism
4.2.1.121 ? x * 55254, calculated from sequence Solanum lycopersicum

Synonyms

EC Number Synonyms Comment Organism
4.2.1.121 CYP74 cytochrome P-450
-
Solanum lycopersicum
4.2.1.121 CYP74D1
-
Solanum lycopersicum
4.2.1.121 LeDES
-
Solanum lycopersicum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.1.121 500
-
(9S,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoate pH 7.0, 25°C Solanum lycopersicum
4.2.1.121 890
-
(9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate pH 7.0, 25°C Solanum lycopersicum

Cofactor

EC Number Cofactor Comment Organism Structure
4.2.1.121 cytochrome P-450 the enzyme has spectral properties of cytochrome P-450 Solanum lycopersicum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.2.1.121 10420
-
(9S,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoate pH 7.0, 25°C Solanum lycopersicum
4.2.1.121 13280
-
(9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate pH 7.0, 25°C Solanum lycopersicum