Literature summary extracted from
Gupta, P.; Ghosalkar, A.; Mishra, S.; Chaudhuri, T.K.
Enhancement of over expression and chaperone assisted yield of folded recombinant aconitase in Escherichia coli in bioreactor cultures (2009), J. Biosci. Bioeng., 107, 102-107.
Application
EC Number |
Application |
Comment |
Organism |
---|
4.2.1.3 |
biotechnology |
molecular chaperones GroEL/GroES are co-expressed with soluble, biologically active recombinant aconitase in Escherichia coli by cultivation in a bioreactor at different temperatures under optimized conditions. The yield of functional aconitase is enhanced, either in presence of co-expressed GroEL/ES or at low temperature cultivation. The outcome from the chaperone assisted folding of aconitase is more pronounced at lower temperature |
Saccharomyces cerevisiae |
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
4.2.1.3 |
yeast mitochondrial aconitase is expressed in Escherichia coli by cultivation in a bioreactor at different temperatures. Chaperones GroEL and GroES are co-expressed |
Saccharomyces cerevisiae |
Localization
EC Number |
Localization |
Comment |
Organism |
GeneOntology No. |
Textmining |
---|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
4.2.1.3 |
Saccharomyces cerevisiae |
- |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
4.2.1.3 |
using column chromatography |
Saccharomyces cerevisiae |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
4.2.1.3 |
isocitrate |
- |
Saccharomyces cerevisiae |
cis-aconitate + H2O |
- |
? |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
4.2.1.3 |
aconitase |
- |
Saccharomyces cerevisiae |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
4.2.1.3 |
8 |
- |
assay at |
Saccharomyces cerevisiae |