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Literature summary extracted from

  • Hilbrig, F.; Jerome, V.; Salzig, M.; Freitag, R.
    Strategy for the isolation of native dehydrogenases with potential for biosensor development from the organism Hyphomicrobium zavarzinii ZV580 (2009), J. Chromatogr. A, 1216, 3518-3525.
    View publication on PubMed

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.9.1 12000
-
 two bands appear in an SDS-PAGE: 12 kDa and 42 kDa Hyphomicrobium zavarzinii
1.4.9.1 42000
-
 two bands appear in an SDS-PAGE: 12 kDa and 42 kDa Hyphomicrobium zavarzinii

Organism

EC Number Organism UniProt Comment Textmining
1.4.9.1 Hyphomicrobium zavarzinii
-
 ZV580
-
1.4.9.1 Hyphomicrobium zavarzinii ZV580
-
 ZV580
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.9.1 enzyme is isolated to near purity by a sequence of hydroxyapatite and anion exchange chromatography Hyphomicrobium zavarzinii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.9.1 methylamine + acceptor + H2O
-
 Hyphomicrobium zavarzinii formaldehyde + NH3 + reduced acceptor
-
 ?
1.4.9.1 methylamine + acceptor + H2O
-
 Hyphomicrobium zavarzinii ZV580 formaldehyde + NH3 + reduced acceptor
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.9.1 dimer anion-exchange chromatography and SDS-PAGE, 12000 Da and 42000 Da subunits Hyphomicrobium zavarzinii

Synonyms

EC Number Synonyms Comment Organism
1.4.9.1 methylamine dehydrogenase
-
 Hyphomicrobium zavarzinii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.9.1 30
-
 assay at Hyphomicrobium zavarzinii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.9.1 8
-
 assay at Hyphomicrobium zavarzinii