Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Xu, X.; Zhang, G.; Wang, L.; Ma, B.; Li, C.
    Quantitative analysis on inactivation and reactivation of recombinant glycerol dehydratase from Klebsiella pneumoniae XJPD-Li (2009), J. Mol. Catal. B, 56, 108-114.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.1.30 ATP is critical to the reactivation of glycerol dehydratase Klebsiella pneumoniae
4.2.1.30 coenzyme B12 is critical to the reactivation of glycerol dehydratase Klebsiella pneumoniae

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.30 plasmid PKRX-T bearing the gene of the dhaBCE double digested with EcoR I and Sac I, resulting 2.7 kb fragment containing the intact dhaBCE genes inserted into pET-28a (+) vector linearized with the same enzymes. Transformation of the ligation mixture (pET-28 (+) dhaBCE) into Escherichia coli BL21 (DE3) for overexpression Klebsiella pneumoniae

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.30 glycerol can inactive the glycerol dehydratase expressed in Escherichia coli quickly in 10 min. Can be effectively reactivated to 98.9% by 50 mM ATP, 10 mM Mg2+ and 0.003 mM coenzyme B12, respectively, when the ratio (w/w) of glycerol dehydratase to reactivation factor is 4:1. Activity of glycerol inactivated GDHt is easier to be recovered than that of O2-inactivated GDHt Klebsiella pneumoniae
4.2.1.30 O2 can inactive the glycerol dehydratase expressed in Escherichia coli quickly in 10 min. Can be effectively reactivated to 97.3% by 50 mM ATP, 10 mM Mg2+ and 0.003 mM coenzyme B12, respectively, when the ratio (w/w) of glycerol dehydratase to reactivation factor is 4:1. Activity of glycerol inactivated GDHt is easier to be recovered than that of O2-inactivated GDHt Klebsiella pneumoniae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.30 Mg2+
-
 Klebsiella pneumoniae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.30 16101
-
 x * 60702 + x * 21322 + x * 16101, alphabetagamma, sequence analysis Klebsiella pneumoniae
4.2.1.30 21322
-
 x * 60702 + x * 21322 + x * 16101, alphabetagamma, sequence analysis Klebsiella pneumoniae
4.2.1.30 60702
-
 x * 60702 + x * 21322 + x * 16101, alphabetagamma, sequence analysis Klebsiella pneumoniae

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.30 Klebsiella pneumoniae Q59475 and A6NA24 and A6NA25 Q59475: small subunit, A6NA24: large subunit, A6NA25: small subunit
-
4.2.1.30 Klebsiella pneumoniae XJPD-Li Q59475 and A6NA24 and A6NA25 Q59475: small subunit, A6NA24: large subunit, A6NA25: small subunit
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.30 0.299
-
 recombined glycerol dehydratase Klebsiella pneumoniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.30 1,2-propanediol
-
 Klebsiella pneumoniae propionaldehyde + H2O
-
 ?
4.2.1.30 1,2-propanediol
-
 Klebsiella pneumoniae XJPD-Li propionaldehyde + H2O
-
 ?
4.2.1.30 glycerol
-
 Klebsiella pneumoniae 3-hydroxypropanal + H2O
-
 ?
4.2.1.30 glycerol
-
 Klebsiella pneumoniae XJPD-Li 3-hydroxypropanal + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.30 ? x * 60702 + x * 21322 + x * 16101, alphabetagamma, sequence analysis Klebsiella pneumoniae

Synonyms

EC Number Synonyms Comment Organism
4.2.1.30 DhaBCE
-
 Klebsiella pneumoniae
4.2.1.30 GDHt
-
 Klebsiella pneumoniae
4.2.1.30 glycerol dehydratase
-
 Klebsiella pneumoniae

General Information

EC Number General Information Comment Organism
4.2.1.30 physiological function overexpressed glycerol dehydratase shows higher enzyme activity than that of wild-strain Klebsiella pneumoniae