EC Number | Cloned (Comment) | Organism |
---|---|---|
4.2.1.36 | lys3 from the parental strain Wis 54-1255 and lys3 mutant allele from strain L2, cloned into pBluescript KS(+) | Penicillium chrysogenum |
4.2.1.36 | plasmid pLysF1 expressed in Penicillium chrysogenum L2 mutant strain | Aspergillus nidulans |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.2.1.36 | 84490 | - |
Wis 54-1255 gene lys3, sequence analysis | Penicillium chrysogenum |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.1.36 | Aspergillus nidulans | Q92412 | - |
- |
4.2.1.36 | Penicillium chrysogenum | B1P2N4 | strain Wis 54-1255 and strain L2 | - |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
4.2.1.36 | mycelium | - |
Penicillium chrysogenum | - |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.2.1.36 | Homoaconitase | - |
Penicillium chrysogenum |
4.2.1.36 | Homoaconitase | - |
Aspergillus nidulans |
4.2.1.36 | lysF | - |
Aspergillus nidulans |
EC Number | Organism | Comment | Expression |
---|---|---|---|
4.2.1.36 | Penicillium chrysogenum | transcription of the lys3 gene shows a 3fold increase in the transcript level of this gene in strain L2 as compared with the parental strain Wis 54-1255 | up |
EC Number | General Information | Comment | Organism |
---|---|---|---|
4.2.1.36 | malfunction | L2 mutant is deficient in homoaconitase activity since it is complemented by the Aspergillus nidulans lysF gene. Wis 54-1255 gene lys3 complements the L2 mutation. Accumulation of homocitric acid in the L2 strain, resulting from the mutation in the lys3 (homoaconitase) gene, is required for the upregulation of the lysine biosynthetic genes. The mutant Lys3 protein is altered in a region required to bind the [4Fe-4S] cluster | Penicillium chrysogenum |
4.2.1.36 | physiological function | lysF gene can complement the Penicillium chrysogenum L2 mutant deficient in homoaconitase activity | Aspergillus nidulans |