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Literature summary extracted from

  • Mayer, J.; Huhn, T.; Habeck, M.; Denger, K.; Hollemeyer, K.; Cook, A.M.
    2,3-Dihydroxypropane-1-sulfonate degraded by Cupriavidus pinatubonensis JMP134: purification of dihydroxypropanesulfonate 3-dehydrogenase (2010), Microbiology, 156, 1556-1564.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.308 0.16
-
 NAD+ pH 9.0, 30°C Cupriavidus pinatubonensis
1.1.1.308 0.46
-
 (R)-2,3-dihydroxypropane-1-sulfonate pH 9.0, 30°C Cupriavidus pinatubonensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.308 46881
-
 x * 46881, calculated from sequence Cupriavidus pinatubonensis
1.1.1.308 47000
-
 x * 47000, SDS-PAGE Cupriavidus pinatubonensis
1.1.1.308 75000
-
 gel filtration Cupriavidus pinatubonensis
4.4.1.24 42000
-
 SDS-PAGE Cupriavidus pinatubonensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.308 (R)-2,3-dihydroxypropane-1-sulfonate + 2 NAD+ + H2O Cupriavidus pinatubonensis degradation of (R)-2,3-dihydroxypropanesulfonate (R)-sulfolactate + 2 NADH + 2 H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.308 Cupriavidus pinatubonensis Q46N53 JMP134, i.e. Ralstonia eutropha str. JMP134
-
2.3.3.15 Roseovarius nubinhibens
-
-
-
4.4.1.24 Cupriavidus pinatubonensis
-
-
-
4.4.1.25 Roseovarius nubinhibens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.308
-
 Cupriavidus pinatubonensis
2.3.3.15
-
 Roseovarius nubinhibens
4.4.1.24
-
 Cupriavidus pinatubonensis
4.4.1.25
-
 Roseovarius nubinhibens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.308 4.74
-
-
 Cupriavidus pinatubonensis
2.3.3.15 0.08
-
 crude extract Roseovarius nubinhibens
4.4.1.24 0.016
-
 crude extract Cupriavidus pinatubonensis
4.4.1.25 0.02
-
 crude extract Roseovarius nubinhibens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.308 (R)-2,3-dihydroxypropane-1-sulfonate + 2 NAD+ + H2O degradation of (R)-2,3-dihydroxypropanesulfonate Cupriavidus pinatubonensis (R)-sulfolactate + 2 NADH + 2 H+
-
 ?
2.3.3.15 2-sulfoacetaldehyde + phosphate
-
 Roseovarius nubinhibens acetyl phosphate + sulfite
-
 ?
4.4.1.24 (2R)-3-sulfolactate
-
 Cupriavidus pinatubonensis pyruvate + bisulfite
-
 ?
4.4.1.25 (S)-cysteate + H2O
-
 Roseovarius nubinhibens HSO3- + pyruvate + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.308 homodimer x * 47000, SDS-PAGE Cupriavidus pinatubonensis
1.1.1.308 homodimer x * 46881, calculated from sequence Cupriavidus pinatubonensis

Synonyms

EC Number Synonyms Comment Organism
1.1.1.308 2,3-dihydroxypropane-1-sulfonate 3-dehydrogenase (sulfolactate forming)
-
 Cupriavidus pinatubonensis
1.1.1.308 DHPS 3-dehydrogenase (sulfolactate forming)
-
 Cupriavidus pinatubonensis
1.1.1.308 dihydroxypropanesulfonate 3-dehydrogenase
-
 Cupriavidus pinatubonensis
1.1.1.308 hpsN
-
 Cupriavidus pinatubonensis
2.3.3.15 sulfoacetaldehyde acetyltransferase
-
 Roseovarius nubinhibens
2.3.3.15 Xsc
-
 Roseovarius nubinhibens
4.4.1.24 (R)-sulfolactate sulfo-lyase
-
 Cupriavidus pinatubonensis
4.4.1.24 SuyAB
-
 Cupriavidus pinatubonensis
4.4.1.25 (S)-cysteate sulfo-lyase
-
 Roseovarius nubinhibens
4.4.1.25 CuyA
-
 Roseovarius nubinhibens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.308 30
-
 assay at Cupriavidus pinatubonensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.308 9 10 activity decreases rapidly with decreasing pH. At pH 6.5 the enzyme is almost inactive Cupriavidus pinatubonensis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.308 9 10 pH 9-10: optimum. Activity decreases rapidly with decreasing pH. At pH 6.5 the enzyme is almost inactive Cupriavidus pinatubonensis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.308 NAD+
-
 Cupriavidus pinatubonensis