EC Number | Cloned (Comment) | Organism |
---|---|---|
4.1.1.4 | expressed in Escherichia coli BL21(DE3) cells | Clostridium acetobutylicum |
4.1.1.4 | expressed in Escherichia coli BL21(DE3) cells | Chromobacterium violaceum |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
4.1.1.4 | AAD complexed with 2,4-pentanedione, hanging drop vapor diffusion method, using 18-20% (v/v) glycerol, 40 mM phosphate buffer, pH 5.95, 100 mM sarcosine and 14-15% (w/v) PEG 3350 | Clostridium acetobutylicum |
4.1.1.4 | AAD complexed with 2,4-pentanedione, hanging drop vapor diffusion method, using 18-20% (v/v) glycerol, 40 mM phosphate buffer, pH 5.95, 100 mM sarcosine and 14-15% (w/v) PEG 3350 | Chromobacterium violaceum |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.1.1.4 | E61Q | the catalytic activity of the mutant shows a decrease in kcat (about 20fold with no change in Km) | Clostridium acetobutylicum |
4.1.1.4 | E61Q | the catalytic activity of the mutant shows a decrease in kcat (about 20fold with no change in Km) | Chromobacterium violaceum |
4.1.1.4 | E76Q | the catalytic activity of the mutant shows a decrease in kcat, the mutant exhibits a slight downward shift of the pH optimum | Clostridium acetobutylicum |
4.1.1.4 | E76Q | the catalytic activity of the mutant shows a decrease in kcat, the mutant exhibits aslight downward shift of the pH optimum | Chromobacterium violaceum |
4.1.1.4 | R29Q | the catalytic activity of Arg29Gln does not increase at pH values above the wild type optimum for AAD of about 5.4 | Clostridium acetobutylicum |
4.1.1.4 | R29Q | the catalytic activity of Arg29Gln does not increase at pH values above the wild type optimum for AAD of about 5.4 | Chromobacterium violaceum |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
4.1.1.4 | 2,4-Pentanedione | potent inhibitor | Chromobacterium violaceum | |
4.1.1.4 | 2,4-Pentanedione | potent inhibitor | Clostridium acetobutylicum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.1.4 | 4.1 | - |
acetoacetate | at 25°C, in 50 mM phosphate buffer, pH 5.95 | Clostridium acetobutylicum | |
4.1.1.4 | 5.7 | - |
acetoacetate | at 25°C, in 50 mM phosphate buffer, pH 5.95 | Chromobacterium violaceum |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.1.1.4 | 365000 | - |
- |
Clostridium acetobutylicum |
4.1.1.4 | 365000 | - |
- |
Chromobacterium violaceum |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.1.1.4 | Chromobacterium violaceum | Q7NSA6 | - |
- |
4.1.1.4 | Clostridium acetobutylicum | P23670 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.1.1.4 | ammonium sulfate precipitation, DEAE-Sepharose column chromatography, and S-200 gel filtration | Clostridium acetobutylicum |
4.1.1.4 | ammonium sulfate precipitation, DEAE-Sepharose column chromatography, and S-200 gel filtration | Chromobacterium violaceum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.1.4 | 2,4-pentanedione | - |
Chromobacterium violaceum | ? | - |
? | |
4.1.1.4 | acetoacetate + H+ | - |
Clostridium acetobutylicum | acetone + CO2 | - |
? | |
4.1.1.4 | acetoacetate + H+ | - |
Chromobacterium violaceum | acetone + CO2 | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.1.1.4 | homodecamer | x-ray crystallography | Clostridium acetobutylicum |
4.1.1.4 | homodecamer | x-ray crystallography | Chromobacterium violaceum |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.1.1.4 | AAD | - |
Clostridium acetobutylicum |
4.1.1.4 | AAD | - |
Chromobacterium violaceum |
4.1.1.4 | AADase | - |
Clostridium acetobutylicum |
4.1.1.4 | AADase | - |
Chromobacterium violaceum |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.1.4 | 165 | - |
acetoacetate | at 25°C, in 50 mM phosphate buffer, pH 5.95 | Clostridium acetobutylicum | |
4.1.1.4 | 349 | - |
acetoacetate | at 25°C, in 50 mM phosphate buffer, pH 5.95 | Chromobacterium violaceum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.1.1.4 | 5.4 | - |
- |
Clostridium acetobutylicum |
4.1.1.4 | 5.4 | - |
- |
Chromobacterium violaceum |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.1.4 | 0.0007 | - |
2,4-Pentanedione | at 25°C, in 50 mM phosphate buffer, pH 5.95 | Clostridium acetobutylicum |