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Literature summary extracted from

  • Zhou, J.-M.; Fukushi, Y.; Wollenweber, E.; Ibrahim, R.K.
    Characterization of two O-methyltransferase-like genes in barley and maize (2008), Pharm. Biol., 46, 26-34.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.42 expression in Escherichia coli Hordeum vulgare
2.1.1.42 expression in Escherichia coli Zea mays
2.1.1.175 expression in Escherichia coli Hordeum vulgare
2.1.1.175 expression in Escherichia coli Zea mays

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.42 0.00618
-
5,7,3',4'-tetrahydroxyflavone 30°C Hordeum vulgare
2.1.1.42 0.0159
-
5,7,3',4'-tetrahydroxyflavone 30°C Zea mays
2.1.1.175 0.00248
-
tricetin 30°C Hordeum vulgare
2.1.1.175 0.0417
-
tricetin 37°C Zea mays

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.42 additional information requires no Mg2+ Hordeum vulgare
2.1.1.42 additional information requires no Mg2+ Zea mays
2.1.1.175 additional information requires no Mg2+ Hordeum vulgare
2.1.1.175 additional information requires no Mg2+ Zea mays

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.42 38700
-
x * 38700, calculated from sequence Hordeum vulgare
2.1.1.42 39600
-
x * 39600, calculated from sequence Zea mays
2.1.1.175 38700
-
x * 38700, calculated from sequence Hordeum vulgare
2.1.1.175 39600
-
x * 39600, calculated from sequence Zea mays

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.42 Hordeum vulgare
-
-
-
2.1.1.42 Zea mays
-
-
-
2.1.1.175 Hordeum vulgare
-
-
-
2.1.1.175 Zea mays
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.42
-
Hordeum vulgare
2.1.1.42
-
Zea mays
2.1.1.175
-
Hordeum vulgare
2.1.1.175
-
Zea mays

Storage Stability

EC Number Storage Stability Organism
2.1.1.42 4°C, stable when stored in buffer containing 10% (v/v) glycerol for 3-4 weeks Zea mays
2.1.1.42 4°C, stable when stored in buffer containing 10% (v/v) glycerol for 3–4 weeks Hordeum vulgare
2.1.1.175 4°C, stable when stored in buffer containing 10% (v/v) glycerol for 3-4 weeks Hordeum vulgare
2.1.1.175 4°C, stable when stored in buffer containing 10% (v/v) glycerol for 3–4 weeks Zea mays

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.42 S-adenosyl-L-methionine + 5,7,3',4'-tetrahydroxyflavone i.e. luteolin, the enzyme also catalyzes the methylation of tricetin and the methylation of quercetin, prefers tricetin over luteolin Hordeum vulgare S-adenosyl-L-homocysteine + 3'-O-methylluteolin
-
?
2.1.1.42 S-adenosyl-L-methionine + 5,7,3',4'-tetrahydroxyflavone i.e. lutepolin, the enzyme also catalyzes the methylation of tricetin and the methylation of 5-hydroxyferulic acid and quercetin, prefers tricetin over luteolin Zea mays S-adenosyl-L-homocysteine + 3'-O-methylluteolin
-
?
2.1.1.175 2 S-adenosyl-L-methionine + tricetin stepwise methylation of tricetin to its 3'-mono- and 3',5'-dimethyl ethers (major product). The enzyme also catalyzes the 3'-O-methylation of luteolin (EC 2.1.1.42) and the methylation of 5-hydroxyferulic acid and quercetin, prefers tricetin over luteolin Hordeum vulgare 2 S-adenosyl-L-homocysteine + 3',5'-O-dimethyltricetin
-
?
2.1.1.175 2 S-adenosyl-L-methionine + tricetin the enzyme also catalyzes the 3'-O-methylation of luteolin (EC 2.1.1.42) and the methylation of 5-hydroxyferulic acid and quercetin, prefers tricetin over luteolin Zea mays 2 S-adenosyl-L-homocysteine + 3',5'-O-dimethyltricetin
-
?
2.1.1.175 S-adenosyl-L-methionine + 3'-O-methyltricetin the enzyme catalyzes the stepwise methylation of tricetin to its 3'-mono- and 3',5'-dimethyl ethers (major product). In contrast with the wheat enzyme, tricetin dimethyl ether is not converted to its 3',4',5'-trimethylated ester derivatives Hordeum vulgare S-adenosyl-L-homocysteine + 3',5'-O-dimethyltricetin
-
?
2.1.1.175 S-adenosyl-L-methionine + 3'-O-methyltricetin the enzyme catalyzes the stepwise methylation of tricetin to its 3'-mono- and 3',5'-dimethyl ethers (major product). In contrast with the wheat enzyme, tricetin dimethyl ether is not converted to its 3',4',5'-trimethylated ester derivatives Zea mays S-adenosyl-L-homocysteine + 3',5'-O-dimethyltricetin
-
?
2.1.1.175 S-adenosyl-L-methionine + tricetin the enzyme catalyzes the stepwise methylation of tricetin to its 3'-mono- and 3',5'-dimethyl ethers (major product). In contrast with the wheat enzyme, tricetin dimethyl ether is not converted to its 3',4',5'-trimethylated ester derivatives Hordeum vulgare S-adenosyl-L-homocysteine + 3'-O-methyltricetin
-
?
2.1.1.175 S-adenosyl-L-methionine + tricetin the enzyme catalyzes the stepwise methylation of tricetin to its 3'-mono- and 3',5'-dimethyl ethers (major product). In contrast with the wheat enzyme, tricetin dimethyl ether is not converted to its 3',4',5'-trimethylated ester derivatives Zea mays S-adenosyl-L-homocysteine + 3'-O-methyltricetin
-
?

Subunits

EC Number Subunits Comment Organism
2.1.1.42 ? x * 38700, calculated from sequence Hordeum vulgare
2.1.1.42 ? x * 39600, calculated from sequence Zea mays
2.1.1.175 ? x * 38700, calculated from sequence Hordeum vulgare
2.1.1.175 ? x * 39600, calculated from sequence Zea mays

Synonyms

EC Number Synonyms Comment Organism
2.1.1.42 HvOMT1
-
Hordeum vulgare
2.1.1.42 ZmOMT1
-
Zea mays
2.1.1.175 HvOMT1
-
Hordeum vulgare
2.1.1.175 ZmOMT1
-
Zea mays

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.42 30
-
assay at Hordeum vulgare
2.1.1.42 30
-
assay at Zea mays
2.1.1.175 30
-
assay at Hordeum vulgare
2.1.1.175 30
-
assay at Zea mays

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.42 7.5
-
-
Hordeum vulgare
2.1.1.42 7.5
-
-
Zea mays
2.1.1.175 7.5
-
-
Hordeum vulgare
2.1.1.175 7.5
-
-
Zea mays

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.1.1.42 Zea mays calculated from sequence
-
5.48
2.1.1.42 Hordeum vulgare calculated from sequence
-
5.64
2.1.1.175 Zea mays calculated from sequence
-
5.48
2.1.1.175 Hordeum vulgare calculated from sequence
-
5.64