EC Number | Cloned (Comment) | Organism |
---|---|---|
2.1.1.42 | expression in Escherichia coli | Hordeum vulgare |
2.1.1.42 | expression in Escherichia coli | Zea mays |
2.1.1.175 | expression in Escherichia coli | Hordeum vulgare |
2.1.1.175 | expression in Escherichia coli | Zea mays |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.1.42 | 0.00618 | - |
5,7,3',4'-tetrahydroxyflavone | 30°C | Hordeum vulgare | |
2.1.1.42 | 0.0159 | - |
5,7,3',4'-tetrahydroxyflavone | 30°C | Zea mays | |
2.1.1.175 | 0.00248 | - |
tricetin | 30°C | Hordeum vulgare | |
2.1.1.175 | 0.0417 | - |
tricetin | 37°C | Zea mays |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.1.1.42 | additional information | requires no Mg2+ | Hordeum vulgare | |
2.1.1.42 | additional information | requires no Mg2+ | Zea mays | |
2.1.1.175 | additional information | requires no Mg2+ | Hordeum vulgare | |
2.1.1.175 | additional information | requires no Mg2+ | Zea mays |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.1.1.42 | 38700 | - |
x * 38700, calculated from sequence | Hordeum vulgare |
2.1.1.42 | 39600 | - |
x * 39600, calculated from sequence | Zea mays |
2.1.1.175 | 38700 | - |
x * 38700, calculated from sequence | Hordeum vulgare |
2.1.1.175 | 39600 | - |
x * 39600, calculated from sequence | Zea mays |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.1.42 | Hordeum vulgare | - |
- |
- |
2.1.1.42 | Zea mays | - |
- |
- |
2.1.1.175 | Hordeum vulgare | - |
- |
- |
2.1.1.175 | Zea mays | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.1.1.42 | - |
Hordeum vulgare |
2.1.1.42 | - |
Zea mays |
2.1.1.175 | - |
Hordeum vulgare |
2.1.1.175 | - |
Zea mays |
EC Number | Storage Stability | Organism |
---|---|---|
2.1.1.42 | 4°C, stable when stored in buffer containing 10% (v/v) glycerol for 3-4 weeks | Zea mays |
2.1.1.42 | 4°C, stable when stored in buffer containing 10% (v/v) glycerol for 34 weeks | Hordeum vulgare |
2.1.1.175 | 4°C, stable when stored in buffer containing 10% (v/v) glycerol for 3-4 weeks | Hordeum vulgare |
2.1.1.175 | 4°C, stable when stored in buffer containing 10% (v/v) glycerol for 34 weeks | Zea mays |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.42 | S-adenosyl-L-methionine + 5,7,3',4'-tetrahydroxyflavone | i.e. luteolin, the enzyme also catalyzes the methylation of tricetin and the methylation of quercetin, prefers tricetin over luteolin | Hordeum vulgare | S-adenosyl-L-homocysteine + 3'-O-methylluteolin | - |
? | |
2.1.1.42 | S-adenosyl-L-methionine + 5,7,3',4'-tetrahydroxyflavone | i.e. lutepolin, the enzyme also catalyzes the methylation of tricetin and the methylation of 5-hydroxyferulic acid and quercetin, prefers tricetin over luteolin | Zea mays | S-adenosyl-L-homocysteine + 3'-O-methylluteolin | - |
? | |
2.1.1.175 | 2 S-adenosyl-L-methionine + tricetin | stepwise methylation of tricetin to its 3'-mono- and 3',5'-dimethyl ethers (major product). The enzyme also catalyzes the 3'-O-methylation of luteolin (EC 2.1.1.42) and the methylation of 5-hydroxyferulic acid and quercetin, prefers tricetin over luteolin | Hordeum vulgare | 2 S-adenosyl-L-homocysteine + 3',5'-O-dimethyltricetin | - |
? | |
2.1.1.175 | 2 S-adenosyl-L-methionine + tricetin | the enzyme also catalyzes the 3'-O-methylation of luteolin (EC 2.1.1.42) and the methylation of 5-hydroxyferulic acid and quercetin, prefers tricetin over luteolin | Zea mays | 2 S-adenosyl-L-homocysteine + 3',5'-O-dimethyltricetin | - |
? | |
2.1.1.175 | S-adenosyl-L-methionine + 3'-O-methyltricetin | the enzyme catalyzes the stepwise methylation of tricetin to its 3'-mono- and 3',5'-dimethyl ethers (major product). In contrast with the wheat enzyme, tricetin dimethyl ether is not converted to its 3',4',5'-trimethylated ester derivatives | Hordeum vulgare | S-adenosyl-L-homocysteine + 3',5'-O-dimethyltricetin | - |
? | |
2.1.1.175 | S-adenosyl-L-methionine + 3'-O-methyltricetin | the enzyme catalyzes the stepwise methylation of tricetin to its 3'-mono- and 3',5'-dimethyl ethers (major product). In contrast with the wheat enzyme, tricetin dimethyl ether is not converted to its 3',4',5'-trimethylated ester derivatives | Zea mays | S-adenosyl-L-homocysteine + 3',5'-O-dimethyltricetin | - |
? | |
2.1.1.175 | S-adenosyl-L-methionine + tricetin | the enzyme catalyzes the stepwise methylation of tricetin to its 3'-mono- and 3',5'-dimethyl ethers (major product). In contrast with the wheat enzyme, tricetin dimethyl ether is not converted to its 3',4',5'-trimethylated ester derivatives | Hordeum vulgare | S-adenosyl-L-homocysteine + 3'-O-methyltricetin | - |
? | |
2.1.1.175 | S-adenosyl-L-methionine + tricetin | the enzyme catalyzes the stepwise methylation of tricetin to its 3'-mono- and 3',5'-dimethyl ethers (major product). In contrast with the wheat enzyme, tricetin dimethyl ether is not converted to its 3',4',5'-trimethylated ester derivatives | Zea mays | S-adenosyl-L-homocysteine + 3'-O-methyltricetin | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.1.1.42 | ? | x * 38700, calculated from sequence | Hordeum vulgare |
2.1.1.42 | ? | x * 39600, calculated from sequence | Zea mays |
2.1.1.175 | ? | x * 38700, calculated from sequence | Hordeum vulgare |
2.1.1.175 | ? | x * 39600, calculated from sequence | Zea mays |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.1.1.42 | HvOMT1 | - |
Hordeum vulgare |
2.1.1.42 | ZmOMT1 | - |
Zea mays |
2.1.1.175 | HvOMT1 | - |
Hordeum vulgare |
2.1.1.175 | ZmOMT1 | - |
Zea mays |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.1.1.42 | 30 | - |
assay at | Hordeum vulgare |
2.1.1.42 | 30 | - |
assay at | Zea mays |
2.1.1.175 | 30 | - |
assay at | Hordeum vulgare |
2.1.1.175 | 30 | - |
assay at | Zea mays |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.1.1.42 | 7.5 | - |
- |
Hordeum vulgare |
2.1.1.42 | 7.5 | - |
- |
Zea mays |
2.1.1.175 | 7.5 | - |
- |
Hordeum vulgare |
2.1.1.175 | 7.5 | - |
- |
Zea mays |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
2.1.1.42 | Zea mays | calculated from sequence | - |
5.48 |
2.1.1.42 | Hordeum vulgare | calculated from sequence | - |
5.64 |
2.1.1.175 | Zea mays | calculated from sequence | - |
5.48 |
2.1.1.175 | Hordeum vulgare | calculated from sequence | - |
5.64 |