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Literature summary extracted from

  • Pinto, M.S.; Siqueira, F.P.; Oliveira, A.E.; Fernandes, K.V.
    A wounding-induced PPO from cowpea (Vigna unguiculata) seedlings (2008), Phytochemistry, 69, 2297-2302.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.10.3.1 SDS enzyme is optimally activated by 10 mM SDS Vigna unguiculata

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.10.3.1 EDTA 10 mM, 15% inhibition Vigna unguiculata
1.10.3.1 resorcinol 10 mM, 40% inhibition Vigna unguiculata
1.10.3.1 Sodium azide 10 mM, 100% inhibition Vigna unguiculata
1.10.3.1 tropolone most powerful specific PPO inhibitor. It reduces the PPO activity by 50%, when used at a low 0.01 mM concentration Vigna unguiculata

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.1 3.44
-
 4-methylcatechol
-
 Vigna unguiculata
1.10.3.1 9.86
-
 4-methylcatechol
-
 Vigna unguiculata

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.10.3.1 58000
-
 by partially denaturing SDS-PAGE multiple molecular forms of active PPO are detected Vigna unguiculata
1.10.3.1 73000
-
 by partially denaturing SDS-PAGE multiple molecular forms of active PPO are detected Vigna unguiculata
1.10.3.1 220000
-
 by partially denaturing SDS-PAGE multiple molecular forms of active PPO are detected Vigna unguiculata

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.1 Vigna unguiculata
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.1 using (NH4)2SO4 precipitation and ion-exchange chromatography Vigna unguiculata

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.10.3.1 seedling polyphenol oxidases are induced in cowpea plants by wounding Vigna unguiculata
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.10.3.1 additional information
-
 polyphenol oxidases are induced in cowpea plants by wounding. The highest activity levels are detected 48 h after this stimulus in both wounded and neighbor-to-wounded unifoliates of cowpea seedlings. The increase of activity is in the order of 13 to 15fold, respectively, in comparison to control unifoliates Vigna unguiculata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.1 (-)-catechin + O2 enzyme activity relative to 4-methylcatechol: 0.77% Vigna unguiculata ?
-
 ?
1.10.3.1 2 catechol + O2 enzyme activity relative to 4-methylcatechol: 87% Vigna unguiculata 2 1,2-benzoquinone + 2 H2O
-
 ?
1.10.3.1 3-(3,4-dihydroxyphenyl)-L-alanine + O2 enzyme activity relative to 4-methylcatechol: 1.15% Vigna unguiculata ?
-
 ?
1.10.3.1 4-methylcatechol + O2 enzyme shows highest activity with 4-methylcatechol Vigna unguiculata ?
-
 ?
1.10.3.1 chlorogenic acid + O2 enzyme activity relative to 4-methylcatechol: 6.15% Vigna unguiculata ?
-
 ?
1.10.3.1 pyrogallol + O2 enzyme activity relative to 4-methylcatechol: 9.24% Vigna unguiculata ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.10.3.1 multimer by partially denaturing SDS-PAGE multiple molecular forms of active PPO are detected: 58 kDa, 73 kDa and 220 kDa Vigna unguiculata

Synonyms

EC Number Synonyms Comment Organism
1.10.3.1 polyphenol oxidase
-
 Vigna unguiculata
1.10.3.1 PPO
-
 Vigna unguiculata

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.10.3.1 80
-
 enzyme is highly stable even 5 min at 80°C Vigna unguiculata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.1 6
-
 assay at Vigna unguiculata
1.10.3.1 6
-
 optimal pH using 4-methylcatechol as substrate Vigna unguiculata

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.10.3.1 5.5 8
-
 Vigna unguiculata

General Information

EC Number General Information Comment Organism
1.10.3.1 physiological function polyphenol oxidases are induced in cowpea plants by wounding Vigna unguiculata