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Literature summary extracted from

  • Kim, E.J.; Lee, J.K.
    Competitive inhibitions of the chlorophyll synthase of Synechocystis sp. strain PCC 6803 by bacteriochlorophyllide a and the bacteriochlorophyll synthase of Rhodobacter sphaeroides by chlorophyllide a (2010), J. Bacteriol., 192, 198-207.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.62 PCR-amplification, in T-vector, expression plasmid pRSET-A in Escherichia coli BL21(DE3)/pLysS Synechocystis sp. PCC 6803

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.62 bacteriochlorophyllide a competitive inhibitor, effect is reversed by an increased level of chlroophyllide a, no cell growth of 50 microM Synechocystis sp. PCC 6803

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.62 0.1
-
 geranylgeranyl diphosphate lysate mixed into reaction buffer (120 mM potassium acetate, 10 mM magnesium acetate, 50 mM HEPES-KOH, pH 7.6, 14 mM 2-mercaptoethanol, 10% glycerol, and 0.5 mM ATP), 30°C Synechocystis sp. PCC 6803
2.5.1.62 0.32
-
 phytyl diphosphate lysate mixed into reaction buffer (120 mM potassium acetate, 10 mM magnesium acetate, 50 mM HEPES-KOH, pH 7.6, 14 mM 2-mercaptoethanol, 10% glycerol, and 0.5 mM ATP), 30°C Synechocystis sp. PCC 6803

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.62 chlorophyllide a + geranylgeranyl diphosphate Synechocystis sp. PCC 6803
-
 geranylgeranylchlorophyllide a + diphosphate
-
 ?
2.5.1.62 chlorophyllide a + phytyl diphosphate Synechocystis sp. PCC 6803
-
 chlorophyll a + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.62 Synechocystis sp. PCC 6803
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.62 cell harvesting, washing with 10 mM Tris-HCl, pH 8.0, freeze-thaw-cycles, incubated with DNase and 5 mM MgCl2 at room temperature, lysate used for assays Synechocystis sp. PCC 6803

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.62 chlorophyllide a + geranylgeranyl diphosphate
-
 Synechocystis sp. PCC 6803 geranylgeranylchlorophyllide a + diphosphate
-
 ?
2.5.1.62 chlorophyllide a + phytyl diphosphate
-
 Synechocystis sp. PCC 6803 chlorophyll a + diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.62 ChlG
-
 Synechocystis sp. PCC 6803
2.5.1.62 chlorophyll synthase
-
 Synechocystis sp. PCC 6803

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.5.1.62 0.3
-
 bacteriochlorophyllide a in the presence of sufficient geranylgeranyl diphosphate as substrate, lysate mixed into reaction buffer (120 mM potassium acetate, 10 mM magnesium acetate, 50 mM HEPES-KOH, pH 7.6, 14 mM 2-mercaptoethanol, 10% glycerol, and 0.5 mM ATP), 30°C Synechocystis sp. PCC 6803
2.5.1.62 1.14
-
 bacteriochlorophyllide a in the presence of sufficient phytyl diphosphate as substrate, lysate mixed into reaction buffer (120 mM potassium acetate, 10 mM magnesium acetate, 50 mM HEPES-KOH, pH 7.6, 14 mM 2-mercaptoethanol, 10% glycerol, and 0.5 mM ATP), 30°C Synechocystis sp. PCC 6803