Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • da Silva, T.M.; Maller, A.; Damasio, A.R.; Michelin, M.; Ward, R.J.; Hirata, I.Y.; Jorge, J.A.; Terenzi, H.F.; de Polizeli, M.L.
    Properties of a purified thermostable glucoamylase from Aspergillus niveus (2009), J. Ind. Microbiol. Biotechnol., 36, 1439-1446.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.3 beta-mercaptoethanol 1 mM Aspergillus niveus
3.2.1.3 EDTA 1 mM Aspergillus niveus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.3 Ag+ 21% inhibition of enzyme activity Aspergillus niveus
3.2.1.3 Fe2+ 5% inhibition of enzyme activity Aspergillus niveus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.3 additional information
-
 starch 0.32 mg/ml Aspergillus niveus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.3 BaCl2 1 mM Aspergillus niveus
3.2.1.3 CaCl2 1 mM Aspergillus niveus
3.2.1.3 CoCl2 1 mM Aspergillus niveus
3.2.1.3 CuCl2 1 mM Aspergillus niveus
3.2.1.3 KCl 1 mM Aspergillus niveus
3.2.1.3 KH2PO4 1 mM Aspergillus niveus
3.2.1.3 MgCl2 1 mM Aspergillus niveus
3.2.1.3 MnCl2 1 mM Aspergillus niveus
3.2.1.3 NaBr 1 mM Aspergillus niveus
3.2.1.3 NaCl 1 mM Aspergillus niveus
3.2.1.3 NaH2PO4 1 mM Aspergillus niveus
3.2.1.3 NH4Cl 1 mM Aspergillus niveus
3.2.1.3 NH4F 1 mM Aspergillus niveus
3.2.1.3 ZnCl2 1 mM Aspergillus niveus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.3 76000
-
 Bio-Sil-Sec-400 gel filtration Aspergillus niveus
3.2.1.3 77000
-
 SDS-PAGE Aspergillus niveus

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.2.1.3 Acetone 10%, enzyme remains competely active Aspergillus niveus
3.2.1.3 Ethanol 10%, enzyme maintains high activtiy Aspergillus niveus
3.2.1.3 isopropanol 10%, enzyme maintains high activity Aspergillus niveus
3.2.1.3 Methanol 10%, enzyme remains completely active Aspergillus niveus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.3 Aspergillus niveus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.3 2.53fold by DEAE-Fractogel and Concanacalin A-Sepharose chromatography Aspergillus niveus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.3 600
-
 after purification Aspergillus niveus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.3 starch + H2O
-
 Aspergillus niveus D-glucose + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.3 1,4-alpha-D-glucan-glucohydrolase
-
 Aspergillus niveus
3.2.1.3 glucoamylase
-
 Aspergillus niveus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.3 65
-
-
 Aspergillus niveus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.3 60
-
 100% enzyme activity after 240 min at 60°C Aspergillus niveus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.3 14.2
-
 starch
-
 Aspergillus niveus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.3 5 5.5
-
 Aspergillus niveus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.3 4 9.5 enzyme remains stable for at least 2 h Aspergillus niveus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.3 Aspergillus niveus
-
-
 3.8

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.3 additional information
-
 starch 44.4 ml/sec*mg Aspergillus niveus