Literature summary extracted from

Jamin, Y.; Gabellieri, C.; Smyth, L.; Reynolds, S.; Robinson, S.P.; Springer, C.J.; Leach, M.O.; Payne, G.S.; Eykyn, T.R.
Hyperpolarized (13)C magnetic resonance detection of carboxypeptidase G2 activity (2009), Magn. Reson. Med., 62, 1300-1304.

Application

EC Number	Application	Comment	Organism
3.4.17.11	medicine	carboxypeptidase G2 is a bacterial enzyme that is currently employed in a range of targeted cancer chemotherapy strategies such as gene-directed enzyme prodrug therapy (GDEPT). These therapeutic strategies would benefit from robust imaging strategies that afford high spatial and temporal resolution images of the biodistribution of carboxypeptidase G2 activity. Employing dynamic nuclear polarization (DNP) and natural abundance 13C magnetic resonance spectroscopy (MRS), the carboxypeptidase G2-mediated conversion of a novel hyperpolarized reporter probe 3,5-difluorobenzoyl-L-glutamic acid to 3,5-difluorobenzoic acid and L-glutamic acid is observed in vitro. The potential for translation in vivo will primarily depend on the 13C relaxation times and this appears to be an important limiting factor in the case of 3,5-difluorobenzoyl-L-glutamic acid	Pseudomonas sp.

Localization

EC Number	Localization	Comment	Organism	GeneOntology No.	Textmining
3.4.17.11	extracellular	-	Pseudomonas sp.	-	-

Metals/Ions

EC Number	Metals/Ions	Comment	Organism	Structure
3.4.17.11	Zn2+	-	Pseudomonas sp.

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.4.17.11	Pseudomonas sp.	-	-	-

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining
3.4.17.11	commercial preparation	-	Pseudomonas sp.	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3.4.17.11	3,5-difluorobenzoyl-L-glutamic acid + H2O	-	Pseudomonas sp.	3,5-difluorobenzoic acid + L-glutamate	-	?

Synonyms

EC Number	Synonyms	Comment	Organism
3.4.17.11	carboxypeptidase G2	-	Pseudomonas sp.
3.4.17.11	CPG2	-	Pseudomonas sp.

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Release 2023.1 (January 2023)