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Literature summary extracted from

  • Shalygo, N.; Czarnecki, O.; Peter, E.; Grimm, B.
    Expression of chlorophyll synthase is also involved in feedback-control of chlorophyll biosynthesis (2009), Plant Mol. Biol., 71, 425-436.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.62 subcloning in the pGEM-T vector, cloning in the pBirAR vector for overexpression or gene silencing, transformation of Nicotiana tabacum leaf discs mediated by Agrobacterium tumefaciens strain GV2260 Nicotiana tabacum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.5.1.62 chloroplast
-
 Nicotiana tabacum 9507
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.62 40000
-
 calculated Nicotiana tabacum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.62 chlorophyllide a + geranylgeranyl diphosphate Nicotiana tabacum
-
 geranylgeranylchlorophyllide a + diphosphate
-
 ?
2.5.1.62 chlorophyllide a + phytyl diphosphate Nicotiana tabacum
-
 chlorophyll a + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.62 Nicotiana tabacum C3W4Q1 Nicotiana tabacum cv. Samsun NN, transgenic tobacco plants
-
2.5.1.62 Nicotiana tabacum C3W4Q2 Nicotiana tabacum cv. Samsun NN, transgenic tobacco plants
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.62 leaf
-
 Nicotiana tabacum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.62 additional information
-
 leaf segments are laid out on buffer-moistened filter paper containing Zn chlorophyllide a for 8 h in the dark, formed product is analyzed by HPLC, overexpression leads to 120-140% chlorophyll synthase activity, expression of the antisense RNA reduces activity to less than 20% and 40% Nicotiana tabacum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.62 chlorophyllide a + geranylgeranyl diphosphate
-
 Nicotiana tabacum geranylgeranylchlorophyllide a + diphosphate
-
 ?
2.5.1.62 chlorophyllide a + phytyl diphosphate
-
 Nicotiana tabacum chlorophyll a + diphosphate
-
 ?
2.5.1.62 Zn chlorophyllide a + geranylgeranyl diphosphate
-
 Nicotiana tabacum Zn chlorophyll a + diphosphate
-
 ?
2.5.1.62 Zn chlorophyllide a + phytyl diphosphate
-
 Nicotiana tabacum Zn chlorophyll a + diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.62 chlorophyll synthase
-
 Nicotiana tabacum
2.5.1.62 NtCHLG
-
 Nicotiana tabacum

General Information

EC Number General Information Comment Organism
2.5.1.62 malfunction reduced enzyme activity does not result in accumulation of chlorophyllide, it causes reduced 5-aminolevulinic acid formation and Mg chelatase and ferrochelatase activity, growth is retarded, leaves are pale green (20% wild type chlorophyll content), overexpression is correlated with enhanced 5-aminolevulinic acid synthesizing capacity and more chelatase activity, the results point to a feedback control of the biosynthesis pathway, the phenotype is not distinguishable from the wild type Nicotiana tabacum
2.5.1.62 metabolism enzyme catalyzes the last step in the chlorophyll biosynthetic pathway Nicotiana tabacum