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Literature summary extracted from

  • Jimenez, J.I.; Acebron, I.; Garcia, J.L.; Diaz, E.; Mancheno, J.M.
    A preliminary crystallographic study of recombinant NicX, an Fe2+-dependent 2,5-dihydroxypyridine dioxygenase from Pseudomonas putida KT2440 (2010), Acta Crystallogr. Sect. F, 66, 549-553.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.13.11.9 DTT
-
 Pseudomonas putida

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.9 functional overexpression in Escherichia coli strain BL21 (DE3) Pseudomonas putida

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.13.11.9 purified recombinant NicX, hanging drop vapour diffusion method, 0.001 ml of 14 mg/ml protein in 20 mM HEPES pH 7.5, 0.1 M NaCl, and 2 mM DTT, is mixed with 0.001 ml of reservoir solution, containing 22% w/v PEG 4000, 0.1 M sodium acetate and 0.1 M HEPES pH 7.5, 2 mM DTT, and with 1 ml 50 mM EDTA, 18°C, method optimization, X-ray diffraction structure determination and analysis at 2.0 A resolution Pseudomonas putida

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.9 Fe2+ dependent on Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.9 143200
-
 gel filtration and mass spectrometry Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.9 2,5-dihydroxypyridine + O2 Pseudomonas putida
-
 N-formylmaleamic acid
-
 ?
1.13.11.9 2,5-dihydroxypyridine + O2 Pseudomonas putida KT 2240
-
 N-formylmaleamic acid
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.9 Pseudomonas putida
-
-
-
1.13.11.9 Pseudomonas putida KT 2240
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.9 recombinant enzyme from Escherichia coli strain BL21(DE3) by anion exchange and hydroxyapatite chromatography Pseudomonas putida

Storage Stability

EC Number Storage Stability Organism
1.13.11.9 4°C, purified recombinant enzyme, 20 mM HEPES pH 7.5, 0.1 M NaCl, and 2 mM DTT, maintains its activity for several days Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.9 2,5-dihydroxypyridine + O2
-
 Pseudomonas putida N-formylmaleamic acid
-
 ?
1.13.11.9 2,5-dihydroxypyridine + O2
-
 Pseudomonas putida KT 2240 N-formylmaleamic acid
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.9 hexamer 6 * 38 883, a hexameric assembly composed of two cyclic trimers, gel filtration and mass spectrometry Pseudomonas putida

Synonyms

EC Number Synonyms Comment Organism
1.13.11.9 2,5-DHP dioxygenase
-
 Pseudomonas putida
1.13.11.9 2,5-dihydroxypyridine dioxygenase
-
 Pseudomonas putida
1.13.11.9 NicX
-
 Pseudomonas putida

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.9 25
-
 assay at Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.9 8
-
 assay at Pseudomonas putida