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Literature summary extracted from

  • Huang, S.T.; Teng, C.J.; Lee, Y.H.; Wu, J.Y.; Wang, K.L.; Lin, C.M.
    Design and synthesis of a long-wavelength latent fluorogenic substrate for salicylate hydroxylase: a useful fluorimetric indicator for analyte determination by dehydrogenase-coupled biosensors (2010), Anal. Chem., 82, 7329-7334.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.14.13.1 analysis usage of the enzyme in a fluorescence assay that detects 3-hydroxybutyrate and cholesterol in the nanomolar range and is more sensitive than the current SHL-dehydrogenase amperometric sensors, making it applicable to the construction of a fiber-optic fluorescence biosensor for clinical diagnostic uses Pseudomonas sp.
1.14.13.1 diagnostics usage of the enzyme in a fluorescence assay that detects 3-hydroxybutyrate and cholesterol in the nanomolar range and is more sensitive than the current SHL-dehydrogenase amperometric sensors, making it applicable to the construction of a fiber-optic fluorescence biosensor for clinical diagnostic uses Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.1 0.0016
-
 salicylate pH 8.0, 37°C Pseudomonas sp.
1.14.13.1 0.0042
-
 4-(3-benzothiazol-2-yl-4-cyano-2-oxo-2H-chromen-7-yloxymethyl)-2-hydroxy-benzoic acid pH 8.0, 37°C Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.1 salicylate + O2 + NADH + 2 H+ Pseudomonas sp.
-
 catechol + CO2 + H2O + NAD+
-
 ?
1.14.13.1 salicylate + O2 + NADPH + 2 H+ Pseudomonas sp.
-
 catechol + CO2 + H2O + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.1 Pseudomonas sp.
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.13.1 commercial preparation
-
 Pseudomonas sp.
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.1 4-(3-benzothiazol-2-yl-4-cyano-2-oxo-2H-chromen-7-yloxymethyl)-2-hydroxy-benzoic acid + O2 + NADH + 2 H+ i.e. SHLF, two-step synthesis of a long-wavelength latent fluorogenic substrate SHLF. In the presence of NADH and under aerobic conditions, SHL catalyzes the decarboxylative hydroxylation of SHLF followed by a quinonemethide-type rearrangement reaction concomitant with the ejection of a fluorescence coumarin 2, which is spontaneous and irreversible at physiological temperatures in aqueous media. The fluorescence signal generated by this process is specific and, in the near red spectral region with an emission maximum at 595 nm, is suppressed by salicylic acid Pseudomonas sp. ?
-
 ?
1.14.13.1 salicylate + O2 + NADH + 2 H+
-
 Pseudomonas sp. catechol + CO2 + H2O + NAD+
-
 ?
1.14.13.1 salicylate + O2 + NADPH + 2 H+
-
 Pseudomonas sp. catechol + CO2 + H2O + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.13.1 salicylate hydroxylase
-
 Pseudomonas sp.
1.14.13.1 SHL
-
 Pseudomonas sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.13.1 37
-
 assay at Pseudomonas sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.13.1 0.00075
-
 4-(3-benzothiazol-2-yl-4-cyano-2-oxo-2H-chromen-7-yloxymethyl)-2-hydroxy-benzoic acid pH 8.0, 37°C Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.13.1 8
-
 assay at Pseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.1 NADH
-
 Pseudomonas sp.
1.14.13.1 NADPH
-
 Pseudomonas sp.