Any feedback?
Literature summary extracted from
- Characterization of two methylenedioxy bridge-forming cytochrome P450-dependent enzymes of alkaloid formation in the Mexican prickly poppy Argemone mexicana (2011), Arch. Biochem. Biophys., 507, 186-193.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.19.64 | expressed in Spodoptera frugiperda Sf9 cells | Argemone mexicana |
| 1.14.19.65 | expressed in Spodoptera frugiperda Sf9 cells | Argemone mexicana |
| 1.14.19.68 | expressed in Spodoptera frugiperda Sf9 cells | Argemone mexicana |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.19.64 | 5.2 | - |
(S)-cheilanthifoline | pH 8.0, 30°C, kcat: 100% | Argemone mexicana |  |
| 1.14.19.65 | 1.9 | - |
(S)-scoulerine | pH 8.0, 30°C, kcat: 100% | Argemone mexicana | |
| 1.14.19.65 | 8.1 | - |
(S)-scoulerine | pH 8.0, 30°C, kcat: 29% | Argemone mexicana | |
| 1.14.19.68 | 2.7 | - |
(S)-tetrahydrocolumbamine | pH 8.0, 30°C, kcat: 135% | Argemone mexicana | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.14.19.64 | 57450 | - |
calculated from cDNA | Argemone mexicana |
| 1.14.19.65 | 57450 | - |
calculated from cDNA | Argemone mexicana |
| 1.14.19.68 | 57450 | - |
calculated from cDNA | Argemone mexicana |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.19.64 | Argemone mexicana | B1NF19 | - |
- |
| 1.14.19.65 | Argemone mexicana | B1NF19 | - |
- |
| 1.14.19.68 | Argemone mexicana | B1NF19 | - |
- |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.14.19.64 | leaf | - |
Argemone mexicana | - |
| 1.14.19.64 | plantlet | - |
Argemone mexicana | - |
| 1.14.19.64 | root | highly expressed | Argemone mexicana | - |
| 1.14.19.64 | stem | - |
Argemone mexicana | - |
| 1.14.19.65 | leaf | - |
Argemone mexicana | - |
| 1.14.19.65 | plantlet | - |
Argemone mexicana | - |
| 1.14.19.65 | root | highly expressed | Argemone mexicana | - |
| 1.14.19.65 | stem | - |
Argemone mexicana | - |
| 1.14.19.68 | leaf | - |
Argemone mexicana | - |
| 1.14.19.68 | plantlet | - |
Argemone mexicana | - |
| 1.14.19.68 | root | highly expressed | Argemone mexicana | - |
| 1.14.19.68 | stem | - |
Argemone mexicana | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.19.64 | (S)-cheilanthifoline + [reduced NADPH-hemoprotein reductase] + O2 | - |
Argemone mexicana | (S)-stylopine + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.19.64 | (S)-coreximine + [reduced NADPH-hemoprotein reductase] + O2 | kcat (S)-coreximine: 1% compared to kcat (S)-cheilanthifoline: 100% | Argemone mexicana | (S)-cheilanthifoline + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.19.65 | (S)-coreximine + [reduced NADPH-hemoprotein reductase] + O2 | kcat (S)-coreximine: 1% compared to kcat (S)-cheilanthifoline: 100% | Argemone mexicana | (S)-cheilanthifoline + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.19.65 | (S)-scoulerine + [reduced NADPH-hemoprotein reductase] + O2 | - |
Argemone mexicana | (S)-cheilanthifoline + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.19.65 | (S)-scoulerine + [reduced NADPH-hemoprotein reductase] + O2 | - |
Argemone mexicana | (S)-nandinine + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.19.68 | (S)-tetrahydrocolumbamine + [reduced NADPH-hemoprotein reductase] + O2 | - |
Argemone mexicana | (S)-canadine + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.19.64 | CYP719A13 | - |
Argemone mexicana |
| 1.14.19.65 | CYP719A13 | - |
Argemone mexicana |
| 1.14.19.65 | CYP719A14 | - |
Argemone mexicana |
| 1.14.19.68 | CYP719A13 | - |
Argemone mexicana |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.19.64 | 30 | - |
assay at | Argemone mexicana |
| 1.14.19.65 | 30 | - |
assay at | Argemone mexicana |
| 1.14.19.68 | 30 | - |
assay at | Argemone mexicana |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.19.64 | 30 | 35 | - |
Argemone mexicana |
| 1.14.19.65 | 30 | 35 | - |
Argemone mexicana |
| 1.14.19.68 | 30 | 35 | - |
Argemone mexicana |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.19.64 | additional information | - |
additional information | kcat (S)-coreximine: 1% compared to kcat (S)-cheilanthifoline: 100% | Argemone mexicana | |
| 1.14.19.65 | additional information | - |
additional information | kcat (S)-coreximine: 1% compared to kcat (S)-cheilanthifoline: 100% | Argemone mexicana | |
| 1.14.19.65 | additional information | - |
additional information | kcat (S)-scoulerine: 100%, pH8, 30°C | Argemone mexicana | |
| 1.14.19.65 | additional information | - |
additional information | kcat (S)-scoulerine: 29%, kcat (S)-cheilanthifoline: 100% | Argemone mexicana |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.19.64 | 8 | - |
assay at, optimal pH using S-cheilanthifoline as a substrate | Argemone mexicana |
| 1.14.19.65 | 7.5 | - |
optimal pH | Argemone mexicana |
| 1.14.19.65 | 8 | - |
assay at | Argemone mexicana |
| 1.14.19.65 | 8 | - |
assay at, optimal pH using S-cheilanthifoline as a substrate | Argemone mexicana |
| 1.14.19.68 | 8 | - |
assay at | Argemone mexicana |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.19.64 | 6 | 10 | half optimal activity at pH 6.5 and pH 9 using S-cheilanthifoline as a substrate | Argemone mexicana |
| 1.14.19.65 | 6 | 10 | half optimal activity at pH 6.5 and 8.5 | Argemone mexicana |
| 1.14.19.65 | 6 | 10 | half optimal activity at pH 6.5 and pH 9 using S-cheilanthifoline as a substrate | Argemone mexicana |
| 1.14.19.68 | 6 | 10 | half optimal activity at pH 6.5 and pH 9 | Argemone mexicana |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 1.14.19.64 | physiological function | CYP719A13 can be involved in both sanguinarine and berberine formation in Argemone mexicana | Argemone mexicana |
| 1.14.19.65 | physiological function | CYP719A13 can be involved in both sanguinarine and berberine formation in Argemone mexicana | Argemone mexicana |
| 1.14.19.68 | physiological function | CYP719A13 can be involved in both sanguinarine and berberine formation in Argemone mexicana | Argemone mexicana |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
