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Literature summary extracted from

  • Zhang, C.; Yang, X.; Feng, J.; Yuan, Y.; Li, X.; Bu, Y.; Xie, Y.; Yuan, H.; Liao, F.
    Effects of modification of amino groups with poly(ethylene glycol) on a recombinant uricase from Bacillus fastidiosus (2010), Biosci. Biotechnol. Biochem., 74, 1298-1301.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.7.3.3 expressed in Escherichia coli BL21(DE3) cells Metabacillus fastidiosus

General Stability

EC Number General Stability Organism
1.7.3.3 after modification with monomethoxy-poly(ethylene glycol)-5000, the recombinant intracellular uricase shows residual activity of about 65% Metabacillus fastidiosus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.3.3 adenine negligible inhibition at 0.5 mM at pH 9.2 Metabacillus fastidiosus
1.7.3.3 Cu2+ strong inhibition at 0.2 mM, negligible inhibition at 0.005 mM Metabacillus fastidiosus
1.7.3.3 Fe2+ strong inhibition at 0.2 mM, negligible inhibition at 0.005 mM Metabacillus fastidiosus
1.7.3.3 hypoxanthine negligible inhibition at 0.5 mM at pH 9.2 Metabacillus fastidiosus
1.7.3.3 oxonate
-
 Metabacillus fastidiosus
1.7.3.3 xanthine
-
 Metabacillus fastidiosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.3.3 0.27
-
 Urate unmodified enzyme, 50 mM borate buffer, 25°C, pH 9.2 Metabacillus fastidiosus
1.7.3.3 0.8
-
 Urate unmodified enzyme, 50 mM borate buffer, 40°C, pH 9.2 Metabacillus fastidiosus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.7.3.3 Ca2+ strong activation at 10 mM Metabacillus fastidiosus
1.7.3.3 K+ slight activation at 10 mM Metabacillus fastidiosus
1.7.3.3 Mg2+ strong activation at 10 mM Metabacillus fastidiosus
1.7.3.3 Na+ slight activation at 10 mM Metabacillus fastidiosus
1.7.3.3 NH4Cl slight activation at 10 mM Metabacillus fastidiosus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.7.3.3 35780
-
 MALDI-TOF mass spectrometry Metabacillus fastidiosus
1.7.3.3 35790
-
 calculated from amino acid sequence Metabacillus fastidiosus

Organism

EC Number Organism UniProt Comment Textmining
1.7.3.3 Metabacillus fastidiosus C5HDG5
-
-
1.7.3.3 Metabacillus fastidiosus ATCC 29604 C5HDG5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.3.3 DEAE-cellulose column chromatography Metabacillus fastidiosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.3.3 urate + O2 + H2O
-
 Metabacillus fastidiosus 5-hydroxyisourate + H2O2
-
 ?
1.7.3.3 urate + O2 + H2O
-
 Metabacillus fastidiosus ATCC 29604 5-hydroxyisourate + H2O2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.7.3.3 uricase
-
 Metabacillus fastidiosus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.7.3.3 40
-
 at 40°C in sodium borate buffer at pH 9.2, the unmodified uricase shows a thermo-inactivation half-life of about 40 h. Modification of the uricase by monomethoxy-poly(ethylene glycol)-350 slightly enhances its thermostability, and modification by monomethoxy-poly(ethylene glycol)-5000 increases its thermo-inactivation half-life to over 85 h at 40°C in sodium borate buffer at pH 9.2 Metabacillus fastidiosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.7.3.3 9.2
-
 the unmodified uricase has a pH optimum of slightly below pH 9.2 which is not altered by modification with NHS esters of monomethoxy-poly(ethylene glycol)-5000 or monomethoxy-poly(ethylene glycol)-350 Metabacillus fastidiosus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.7.3.3 0.008
-
 oxonate unmodified enzyme, 50 mM borate buffer, 25°C, pH 9.2 Metabacillus fastidiosus
1.7.3.3 0.055
-
 xanthine unmodified enzyme, 50 mM borate buffer, 25°C, pH 9.2 Metabacillus fastidiosus
1.7.3.3 0.1
-
 xanthine unmodified enzyme, 50 mM borate buffer, 40°C, pH 9.2 Metabacillus fastidiosus