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Literature summary extracted from

  • Sashidhar, B.; Inampudi, K.K.; Guruprasad, L.; Kondreddy, A.; Gopinath, K.; Podile, A.R.
    Highly conserved Asp-204 and Gly-776 are important for activity of the quinoprotein glucose dehydrogenase of Escherichia coli and for mineral phosphate solubilization (2010), J. Mol. Microbiol. Biotechnol., 18, 109-119.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.5.2 expressed in Escherichia coli AT15 cells Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
1.1.5.2 D204A no GDH activity Escherichia coli
1.1.5.2 E217A no GDH activity Escherichia coli
1.1.5.2 E217L no GDH activity Escherichia coli
1.1.5.2 E217Q the mutant retains its function similar to that of wild type GDH Escherichia coli
1.1.5.2 E591K no GDH activity Escherichia coli
1.1.5.2 E591L no GDH activity Escherichia coli
1.1.5.2 E591Q no GDH activity Escherichia coli
1.1.5.2 G776A the mutant retains its function similar to that of wild type GDH Escherichia coli
1.1.5.2 G776D no GDH activity Escherichia coli
1.1.5.2 G776K no GDH activity Escherichia coli
1.1.5.2 G776L no GDH activity Escherichia coli
1.1.5.2 L712R no GDH activity Escherichia coli
1.1.5.2 L712W no GDH activity Escherichia coli
1.1.5.2 R201A no GDH activity Escherichia coli
1.1.5.2 R266E no GDH activity Escherichia coli
1.1.5.2 R266Q no GDH activity Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.5.2 membrane
-
 Escherichia coli 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.5.2 90000
-
 SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.5.2 D-glucose + ubiquinone Escherichia coli
-
 D-glucono-1,5-lactone + ubiquinol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.5.2 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.5.2 Ni-NTA column chromatography Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.5.2 D-glucose + 2,6-dichloroindophenol
-
 Escherichia coli D-glucono-1,5-lactone + reduced 2,6-dichloroindophenol
-
 ?
1.1.5.2 D-glucose + ubiquinone
-
 Escherichia coli D-glucono-1,5-lactone + ubiquinol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.5.2 GDH
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.5.2 pyrroloquinoline quinone i.e. 2,7,9,-tricarboxyl-1H-pyrrolo[2,3-f]quinoline-4,5-dione Escherichia coli