BRENDA - Enzyme Database show

Spatial organization of the vindoline biosynthetic pathway in Catharanthus roseus

Guirimand, G.; Guihur, A.; Poutrain, P.; Hericourt, F.; Mahroug, S.; St-Pierre, B.; Burlat, V.; Courdavault, V.; J. Plant Physiol. 168, 549-557 (2010)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.14.14.103
DNA and amino acid sequence determination and analysis
Catharanthus roseus
2.1.1.94
-
Catharanthus roseus
2.3.1.107
transient expression of N-terminally or C-terminally GFP-tagged DAT in Catharanthus roseus cells. Irrespective to the fusion orientation, DAT is localized to the cytoplasm and the nucleus, as highlighted by the perfect overlapping of the fluorescence signal of the fusion protein with the nucleocytoplasmic CFP marker
Catharanthus roseus
Engineering
EC Number
Amino acid exchange
Commentary
Organism
2.3.1.107
additional information
analysis of interaction of vindoline biosynthetic enzymes including DAT using yeast two-hybrid assays, overview. DAT does not interact with the other enzymes
Catharanthus roseus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.14.11.20
additional information
vindoline biosynthesis pathway enzyme subcellular localization study, overview
Catharanthus roseus
-
-
1.14.11.20
nucleoplasm
the enzyme resides in the nucleocytoplasmic compartment following passive diffusion to the nucleus allowed by the protein size. Colocalization with deacetylvindoline-4-O-acetyltransferase
Catharanthus roseus
5654
-
1.14.14.103
endoplasmic reticulum
-
Catharanthus roseus
5783
-
1.14.14.103
endoplasmic reticulum membrane
T16H is anchored to the endoplasmic reticulum as a monomer via a putative 18-residue N-terminal helix
Catharanthus roseus
5789
-
1.14.14.103
additional information
vindoline biosynthesis pathway enzyme subcellular localization study, overview
Catharanthus roseus
-
-
2.1.1.94
cytoplasm
-
Catharanthus roseus
5737
-
2.1.1.94
cytosol
-
Catharanthus roseus
5829
-
2.3.1.107
cytoplasm
-
Catharanthus roseus
5737
-
2.3.1.107
additional information
GFP-tagged DAT is localized to the cytoplasm and the nucleus
Catharanthus roseus
-
-
2.3.1.107
nucleus
-
Catharanthus roseus
5634
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.11.20
deacetoxyvindoline + 2-oxoglutarate + O2
Catharanthus roseus
-
deacetylvindoline + succinate + CO2
-
-
?
1.14.11.20
additional information
Catharanthus roseus
the desacetoxyvindoline-4-hydroxylase interacts with the deacetylvindoline-4-O-acetyltransferase, analysis via yeast two-hybrid system
?
-
-
-
1.14.14.103
tabersonine + NADPH + H+ + O2
Catharanthus roseus
-
16-hydroxytabersonine + NADP+ + H2O
-
-
?
2.3.1.107
acetyl-CoA + deacetylvindoline
Catharanthus roseus
-
CoA + vindoline
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.11.20
Catharanthus roseus
-
-
-
1.14.14.103
Catharanthus roseus
C0KYN4
-
-
2.1.1.94
Catharanthus roseus
B0EXJ8
-
-
2.3.1.107
Catharanthus roseus
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.11.20
idioblast
-
Catharanthus roseus
-
1.14.11.20
laticifer
-
Catharanthus roseus
-
1.14.11.20
leaf
-
Catharanthus roseus
-
1.14.14.103
leaf
-
Catharanthus roseus
-
2.1.1.94
cotyledon
-
Catharanthus roseus
-
2.1.1.94
epidermis
specifically located in the epidermis of aerial organs
Catharanthus roseus
-
2.1.1.94
leaf
-
Catharanthus roseus
-
2.3.1.107
leaf
-
Catharanthus roseus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.11.20
deacetoxyvindoline + 2-oxoglutarate + O2
-
712866
Catharanthus roseus
deacetylvindoline + succinate + CO2
-
-
-
?
1.14.11.20
additional information
the desacetoxyvindoline-4-hydroxylase interacts with the deacetylvindoline-4-O-acetyltransferase, analysis via yeast two-hybrid system
712866
Catharanthus roseus
?
-
-
-
-
1.14.14.103
tabersonine + NADPH + H+ + O2
-
712866
Catharanthus roseus
16-hydroxytabersonine + NADP+ + H2O
-
-
-
?
2.1.1.94
S-adenosyl-L-methionine + 16-hydroxytabersonine
-
712866
Catharanthus roseus
S-adenosyl-L-homocysteine + 16-methoxytabersonine
-
-
-
?
2.3.1.107
acetyl-CoA + deacetylvindoline
-
712866
Catharanthus roseus
CoA + vindoline
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.14.11.20
monomer
-
Catharanthus roseus
1.14.14.103
monomer
-
Catharanthus roseus
2.1.1.94
homodimer
-
Catharanthus roseus
2.3.1.107
More
the enzyme is no hetero- or homodimer
Catharanthus roseus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.14.14.103
cytochrome P450
-
Catharanthus roseus
2.3.1.107
acetyl-CoA
-
Catharanthus roseus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.14.103
DNA and amino acid sequence determination and analysis
Catharanthus roseus
2.1.1.94
-
Catharanthus roseus
2.3.1.107
transient expression of N-terminally or C-terminally GFP-tagged DAT in Catharanthus roseus cells. Irrespective to the fusion orientation, DAT is localized to the cytoplasm and the nucleus, as highlighted by the perfect overlapping of the fluorescence signal of the fusion protein with the nucleocytoplasmic CFP marker
Catharanthus roseus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.14.14.103
cytochrome P450
-
Catharanthus roseus
2.3.1.107
acetyl-CoA
-
Catharanthus roseus
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
2.3.1.107
additional information
analysis of interaction of vindoline biosynthetic enzymes including DAT using yeast two-hybrid assays, overview. DAT does not interact with the other enzymes
Catharanthus roseus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.14.11.20
additional information
vindoline biosynthesis pathway enzyme subcellular localization study, overview
Catharanthus roseus
-
-
1.14.11.20
nucleoplasm
the enzyme resides in the nucleocytoplasmic compartment following passive diffusion to the nucleus allowed by the protein size. Colocalization with deacetylvindoline-4-O-acetyltransferase
Catharanthus roseus
5654
-
1.14.14.103
endoplasmic reticulum
-
Catharanthus roseus
5783
-
1.14.14.103
endoplasmic reticulum membrane
T16H is anchored to the endoplasmic reticulum as a monomer via a putative 18-residue N-terminal helix
Catharanthus roseus
5789
-
1.14.14.103
additional information
vindoline biosynthesis pathway enzyme subcellular localization study, overview
Catharanthus roseus
-
-
2.1.1.94
cytoplasm
-
Catharanthus roseus
5737
-
2.1.1.94
cytosol
-
Catharanthus roseus
5829
-
2.3.1.107
cytoplasm
-
Catharanthus roseus
5737
-
2.3.1.107
additional information
GFP-tagged DAT is localized to the cytoplasm and the nucleus
Catharanthus roseus
-
-
2.3.1.107
nucleus
-
Catharanthus roseus
5634
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.11.20
deacetoxyvindoline + 2-oxoglutarate + O2
Catharanthus roseus
-
deacetylvindoline + succinate + CO2
-
-
?
1.14.11.20
additional information
Catharanthus roseus
the desacetoxyvindoline-4-hydroxylase interacts with the deacetylvindoline-4-O-acetyltransferase, analysis via yeast two-hybrid system
?
-
-
-
1.14.14.103
tabersonine + NADPH + H+ + O2
Catharanthus roseus
-
16-hydroxytabersonine + NADP+ + H2O
-
-
?
2.3.1.107
acetyl-CoA + deacetylvindoline
Catharanthus roseus
-
CoA + vindoline
-
-
?
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.11.20
idioblast
-
Catharanthus roseus
-
1.14.11.20
laticifer
-
Catharanthus roseus
-
1.14.11.20
leaf
-
Catharanthus roseus
-
1.14.14.103
leaf
-
Catharanthus roseus
-
2.1.1.94
cotyledon
-
Catharanthus roseus
-
2.1.1.94
epidermis
specifically located in the epidermis of aerial organs
Catharanthus roseus
-
2.1.1.94
leaf
-
Catharanthus roseus
-
2.3.1.107
leaf
-
Catharanthus roseus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.11.20
deacetoxyvindoline + 2-oxoglutarate + O2
-
712866
Catharanthus roseus
deacetylvindoline + succinate + CO2
-
-
-
?
1.14.11.20
additional information
the desacetoxyvindoline-4-hydroxylase interacts with the deacetylvindoline-4-O-acetyltransferase, analysis via yeast two-hybrid system
712866
Catharanthus roseus
?
-
-
-
-
1.14.14.103
tabersonine + NADPH + H+ + O2
-
712866
Catharanthus roseus
16-hydroxytabersonine + NADP+ + H2O
-
-
-
?
2.1.1.94
S-adenosyl-L-methionine + 16-hydroxytabersonine
-
712866
Catharanthus roseus
S-adenosyl-L-homocysteine + 16-methoxytabersonine
-
-
-
?
2.3.1.107
acetyl-CoA + deacetylvindoline
-
712866
Catharanthus roseus
CoA + vindoline
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.14.11.20
monomer
-
Catharanthus roseus
1.14.14.103
monomer
-
Catharanthus roseus
2.1.1.94
homodimer
-
Catharanthus roseus
2.3.1.107
More
the enzyme is no hetero- or homodimer
Catharanthus roseus
General Information
EC Number
General Information
Commentary
Organism
1.14.11.20
metabolism
the enzyme catalyzes the penultimate step in the biosynthesis of vindoline, which constitutes the main terpenoid indole alkaloid accumulated in leaves of Catharanthus roseus, spatial organization of the vindoline biosynthetic pathway, overview. The desacetoxyvindoline-4-hydroxylase interacts directly with the deacetylvindoline-4-O-acetyltransferase, that catalyzes the last step of the biosynthetic pathway, absence of metabolic channeling in the vindoline biosynthetic pathway. Importance of inter- and intracellular translocations of intermediates during the vindoline biosynthesis and potential regulatory role, overview
Catharanthus roseus
1.14.14.103
metabolism
the enzyme catalyzes the first step in the biosynthesis of vindoline, which constitutes the main terpenoid indole alkaloid accumulated in leaves of Catharanthus roseus, spatial organization of the vindoline biosynthetic pathway, overview. Absence of metabolic channeling in the vindoline biosynthetic pathway. Importance of inter- and intracellular translocations of intermediates during the vindoline biosynthesis and potential regulatory role, overview
Catharanthus roseus
2.3.1.107
metabolism
vindoline constitutes the main terpenoid indole alkaloid accumulated in leaves of Catharanthus roseus, spatial tabersonine-to-vindoline biosynthesis organization involving four enzymes for the six-step conversion, overview. Deacetylvindoline-4-O-acetyltransferase catalyzes teh last step of the pathway
Catharanthus roseus
General Information (protein specific)
EC Number
General Information
Commentary
Organism
1.14.11.20
metabolism
the enzyme catalyzes the penultimate step in the biosynthesis of vindoline, which constitutes the main terpenoid indole alkaloid accumulated in leaves of Catharanthus roseus, spatial organization of the vindoline biosynthetic pathway, overview. The desacetoxyvindoline-4-hydroxylase interacts directly with the deacetylvindoline-4-O-acetyltransferase, that catalyzes the last step of the biosynthetic pathway, absence of metabolic channeling in the vindoline biosynthetic pathway. Importance of inter- and intracellular translocations of intermediates during the vindoline biosynthesis and potential regulatory role, overview
Catharanthus roseus
1.14.14.103
metabolism
the enzyme catalyzes the first step in the biosynthesis of vindoline, which constitutes the main terpenoid indole alkaloid accumulated in leaves of Catharanthus roseus, spatial organization of the vindoline biosynthetic pathway, overview. Absence of metabolic channeling in the vindoline biosynthetic pathway. Importance of inter- and intracellular translocations of intermediates during the vindoline biosynthesis and potential regulatory role, overview
Catharanthus roseus
2.3.1.107
metabolism
vindoline constitutes the main terpenoid indole alkaloid accumulated in leaves of Catharanthus roseus, spatial tabersonine-to-vindoline biosynthesis organization involving four enzymes for the six-step conversion, overview. Deacetylvindoline-4-O-acetyltransferase catalyzes teh last step of the pathway
Catharanthus roseus