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Literature summary extracted from

  • Ferreyra, M.L.; Rius, S.; Emiliani, J.; Pourcel, L.; Feller, A.; Morohashi, K.; Casati, P.; Grotewold, E.
    Cloning and characterization of a UV-B-inducible maize flavonol synthase (2010), Plant J., 62, 77-91.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.20.6 gene ZmFLS1, ZmFLS1 is under the control of the anthocyanin (C1/PL1 + R/B) and 3-deoxy flavonoid (P1) transcriptional regulators in vivo, recombinant expression as His-tagged protein in Escherichia coli Zea mays

Protein Variants

EC Number Protein Variants Comment Organism
1.14.20.6 H132F site-directed mutagenesis, the mutant shows similar kinetics with dihydrokaempferol compared to the wild-type enzyme Zea mays
1.14.20.6 additional information ZmFLS1 partially complements the flavonol deficiency of the Arabidopsis thaliana fls1 mutant, and restores anthocyanin accumulation to normal levels. ZmFLS1 is an immediate direct target of the P1 and C1/R regulatory complexes Zea mays

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.20.6 0.0584
-
 dihydrokaempferol recombinant FLS1, pH not specified in the publication, temperature not specified in the publication Zea mays
1.14.20.6 0.1511
-
 dihydroquercetin recombinant FLS1, pH not specified in the publication, temperature not specified in the publication Zea mays

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.20.6 additional information Zea mays FLS1 is capable of converting the dihydrokaempferol and dihydroquercetin dihydroflavonols to the corresponding flavonols, kaempferol and quercetin ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.20.6 Zea mays
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.20.6 recombinant His-tagged FLS1 from Escherichia coli to about 80% purity by nickel affinity chromatography Zea mays

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.20.6 dihydrokaempferol + 2-oxoglutarate + O2
-
 Zea mays kaempferol + succinate + CO2 + H2O
-
 ?
1.14.20.6 dihydroquercetin + 2-oxoglutarate + O2
-
 Zea mays quercetin + succinate + CO2 + H2O
-
 ?
1.14.20.6 additional information FLS1 is capable of converting the dihydrokaempferol and dihydroquercetin dihydroflavonols to the corresponding flavonols, kaempferol and quercetin Zea mays ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.20.6 flavonol synthase
-
 Zea mays
1.14.20.6 FLS1
-
 Zea mays

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.20.6 3.9
-
 dihydroquercetin recombinant FLS1, pH not specified in the publication, temperature not specified in the publication Zea mays
1.14.20.6 6.6
-
 dihydrokaempferol recombinant FLS1, pH not specified in the publication, temperature not specified in the publication Zea mays

Expression

EC Number Organism Comment Expression
1.14.20.6 Zea mays the enzyme is UV-B-light-inducible in maize seedlings up

General Information

EC Number General Information Comment Organism
1.14.20.6 metabolism ZmFLS1 is a key flavonoid biosynthetic enzyme and involved in control of flavonol accumulation in maize Zea mays

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.14.20.6 26
-
 dihydroquercetin recombinant FLS1, pH not specified in the publication, temperature not specified in the publication Zea mays
1.14.20.6 113
-
 dihydrokaempferol recombinant FLS1, pH not specified in the publication, temperature not specified in the publication Zea mays