EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.3.1.85 | 0.2 | - |
CO2 | at pH 7.8, temperature not specified in the publication | Cereibacter sphaeroides | |
1.3.1.85 | 0.2 | - |
(E)-crotonyl-CoA | for reduction, pH and temperature not specified in the publication | Cereibacter sphaeroides | |
1.3.1.85 | 0.4 | - |
(E)-crotonyl-CoA | for reductive carboxylation, pH and temperature not specified in the publication | Cereibacter sphaeroides | |
1.3.1.85 | 0.5 | - |
acryloyl-CoA | pH and temperature not specified in the publication | Cereibacter sphaeroides | |
1.3.1.85 | 0.7 | - |
NADPH | pH and temperature not specified in the publication | Cereibacter sphaeroides |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.3.1.85 | 47000 | - |
2 * 47000, gel filtration | Cereibacter sphaeroides |
1.3.1.85 | 105000 | - |
gel filtration | Cereibacter sphaeroides |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.1.85 | (E)-crotonyl-CoA + CO2 + NADPH + H+ | Cereibacter sphaeroides | 100% relative specific activity, the carboxylation reaction is the physiologically relevant reaction | (2S)-ethylmalonyl-CoA + NADP+ | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.3.1.85 | Cereibacter sphaeroides | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.3.1.85 | - |
Cereibacter sphaeroides |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.1.85 | (E)-crotonyl-CoA + CO2 + NADPH + H+ | 100% relative specific activity, the carboxylation reaction is the physiologically relevant reaction | Cereibacter sphaeroides | (2S)-ethylmalonyl-CoA + NADP+ | - |
r | |
1.3.1.85 | (E)-crotonyl-CoA + NADPH + H+ | 10% of maximal activity (compared with vmax of crotonyl-CoA carboxylation) | Cereibacter sphaeroides | butyryl-CoA + NADP+ | - |
? | |
1.3.1.85 | acryloyl-CoA + CO2 + NADPH + H+ | acryloyl-CoA is accepted as an alternative substrate analogue by the enzyme with 40% relative activity (compared with vmax of crotonyl-CoA carboxylation) | Cereibacter sphaeroides | methylmalonyl-CoA + NADP+ | - |
? | |
1.3.1.85 | additional information | does not reduce crotonyl-N-acetylcysteamine, methacryloyl-CoA, 6-hydroxycylohex-1-ene-1-carboxyl-CoA, cyclo-hexa-1,5-diene-1-carboxyl-CoA, acetoacetyl-CoA, propionyl-CoA, (R)-3-hydroxybutyryl-CoA, (S)-3-hydroxybutyryl-CoA (less than 1% specific activity) | Cereibacter sphaeroides | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.3.1.85 | homodimer | 2 * 47000, gel filtration | Cereibacter sphaeroides |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.3.1.85 | CCR | - |
Cereibacter sphaeroides |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.3.1.85 | 7.5 | 8 | - |
Cereibacter sphaeroides |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.3.1.85 | NADPH | - |
Cereibacter sphaeroides |