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Literature summary extracted from
- Purification and characterization of a novel heparin degrading enzyme from Aspergillus flavus (MTCC-8654) (2010), Appl. Biochem. Biotechnol., 160, 1004-1016.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.2.7 | 2-mercaptoethanol | - |
Aspergillus flavus |  |
| 4.2.2.7 | dithiothreitol | - |
Aspergillus flavus | |
| 4.2.2.7 | EDTA | - |
Aspergillus flavus | |
| 4.2.2.7 | additional information | free sulfohydryl groups of cysteine residues are necessary for catalytic activity of the enzyme | Aspergillus flavus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.2.7 | Ba2+ | - |
Aspergillus flavus | |
| 4.2.2.7 | Cd2+ | - |
Aspergillus flavus | |
| 4.2.2.7 | diethyl dicarbonate | - |
Aspergillus flavus | |
| 4.2.2.7 | iodoacetic acid | - |
Aspergillus flavus | |
| 4.2.2.7 | Pb2+ | - |
Aspergillus flavus | |
| 4.2.2.7 | Zn2+ | - |
Aspergillus flavus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.2.7 | 0.022 | - |
heparin | pH 7.5, 30°C | Aspergillus flavus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 4.2.2.7 | intracellular | - |
Aspergillus flavus | 5622 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.2.7 | Co2+ | activates | Aspergillus flavus | |
| 4.2.2.7 | Mn2+ | activates | Aspergillus flavus | |
| 4.2.2.7 | NaCl | best activating at 300 mM | Aspergillus flavus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.2.2.7 | 23380 | - |
x * 24000, SDS-PAGE, x * 23380, mass spectrometry | Aspergillus flavus |
| 4.2.2.7 | 24000 | - |
x * 24000, SDS-PAGE | Aspergillus flavus |
| 4.2.2.7 | 24000 | - |
x * 24000, SDS-PAGE, x * 23380, mass spectrometry | Aspergillus flavus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.2.7 | additional information | Aspergillus flavus | affinity of the enzyme for different glycosaminoglycans varies, with high substrate specificity toward heparin and heparin-derived polysaccharide. Depolymerization of heparin results in heparin disaccharides as main products | ? | - |
? | |
| 4.2.2.7 | additional information | Aspergillus flavus MTCC-8654 | affinity of the enzyme for different glycosaminoglycans varies, with high substrate specificity toward heparin and heparin-derived polysaccharide. Depolymerization of heparin results in heparin disaccharides as main products | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.2.7 | Aspergillus flavus | - |
- |
- |
| 4.2.2.7 | Aspergillus flavus | - |
phylogenetic tree | - |
| 4.2.2.7 | Aspergillus flavus MTCC-8654 | - |
- |
- |
| 4.2.2.7 | Aspergillus flavus MTCC-8654 | - |
phylogenetic tree | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.2.2.7 | native enzyme 40.5fold by anion and cation exchange chromatography, and gel filtration | Aspergillus flavus |
| 4.2.2.7 | native enzyme 40.5fold by anion exchange and hydrophobic interaction chromatography, and gel filtration | Aspergillus flavus |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.2.2.7 | 44.6 | - |
purified enzyme, pH 7.5, 30°C | Aspergillus flavus |
| 4.2.2.7 | 44.6 | - |
purified enzyme, pH 7.0, 30°C | Aspergillus flavus |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 4.2.2.7 | -20°C, purified native enzyme, 72 h, below 12% loss of activity | Aspergillus flavus |
| 4.2.2.7 | 4°C, purified native enzyme, 72 h, below 22% loss of activity | Aspergillus flavus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.2.7 | heparin | substrate is heparin from porcine intestinal mucosa. Cysteine, histidine may be present at the active site | Aspergillus flavus | heparin disaccharide + ? | - |
? | |
| 4.2.2.7 | heparin | substrate is heparin from porcine intestinal mucosa. Cysteine, histidine may be present at the active site | Aspergillus flavus MTCC-8654 | heparin disaccharide + ? | - |
? | |
| 4.2.2.7 | heparin | main product | Aspergillus flavus | unsaturated heparin disaccharides | - |
? | |
| 4.2.2.7 | heparin | main product | Aspergillus flavus MTCC-8654 | unsaturated heparin disaccharides | - |
? | |
| 4.2.2.7 | additional information | affinity of the enzyme for different glycosaminoglycans varies, with high substrate specificity toward heparin and heparin-derived polysaccharide. Depolymerization of heparin results in heparin disaccharides as main products | Aspergillus flavus | ? | - |
? | |
| 4.2.2.7 | additional information | cysteine, histidine may be present at the active site | Aspergillus flavus | ? | - |
? | |
| 4.2.2.7 | additional information | affinity of the enzyme for different glycosaminoglycans varies, with high substrate specificity toward heparin and heparin-derived polysaccharide. Depolymerization of heparin results in heparin disaccharides as main products | Aspergillus flavus MTCC-8654 | ? | - |
? | |
| 4.2.2.7 | additional information | cysteine, histidine may be present at the active site | Aspergillus flavus MTCC-8654 | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.2.2.7 | ? | x * 24000, SDS-PAGE | Aspergillus flavus |
| 4.2.2.7 | ? | x * 24000, SDS-PAGE, x * 23380, mass spectrometry | Aspergillus flavus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.2.2.7 | heparin degrading enzyme | - |
Aspergillus flavus |
| 4.2.2.7 | Heparinase | - |
Aspergillus flavus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.2.2.7 | 30 | - |
- |
Aspergillus flavus |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.2.2.7 | 15 | 60 | highly active within the temperature range of 25-35°C, but activity is lost rapidly above 35°C | Aspergillus flavus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.2.2.7 | 28 | - |
purified native enzyme, catalytic activity after about 72 h shows a loss of 40.4% in activity | Aspergillus flavus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.2.7 | 7 | - |
- |
Aspergillus flavus |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.2.7 | 6 | 8.5 | high activity range | Aspergillus flavus |
Expression
| EC Number | Organism | Comment | Expression |
|---|---|---|---|
| 4.2.2.7 | Aspergillus flavus | inducible heparinase | up |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 4.2.2.7 | additional information | free sulfohydryl groups of cysteine residues are necessary for catalytic activity of the enzyme | Aspergillus flavus |
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