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Literature summary extracted from

  • Ferreira, J.G.; Reis, A.P.; Guimaraes, V.M.; Falkoski, D.L.; Fialho, L.d.a..S.; de Rezende, S.T.
    Purification and characterization of Aspergillus terreus alpha-galactosidases and their use for hydrolysis of soymilk oligosaccharides (2011), Appl. Biochem. Biotechnol., 164, 1111-1125.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.22 Ag+ inhibits enzymatic activity Aspergillus terreus
3.2.1.22 Cu2+ inhibits enzymatic activity Aspergillus terreus
3.2.1.22 D-galactose inhibits enzymatic activity Aspergillus terreus
3.2.1.22 Hg2+ inhibits enzymatic activity Aspergillus terreus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.22 0.2
-
 4-nitrophenyl-alpha-D-galactopyranoside pH 5, 40°C Aspergillus terreus
3.2.1.22 0.66
-
 4-nitrophenyl-alpha-D-galactopyranoside pH 5, 40°C Aspergillus terreus
3.2.1.22 0.72
-
 4-nitrophenyl-alpha-D-galactopyranoside pH 5, 40°C Aspergillus terreus
3.2.1.22 1.92
-
 melibiose pH 5, 40°C Aspergillus terreus
3.2.1.22 7.39
-
 melibiose pH 5, 40°C Aspergillus terreus
3.2.1.22 10.94
-
 stachyose pH 5, 40°C Aspergillus terreus
3.2.1.22 20.14
-
 melibiose pH 5, 40°C Aspergillus terreus
3.2.1.22 27.93
-
 raffinose pH 5, 40°C Aspergillus terreus
3.2.1.22 32.99
-
 raffinose pH 5, 40°C Aspergillus terreus
3.2.1.22 54.74
-
 stachyose pH 5, 40°C Aspergillus terreus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.22 Ag+ inhibits enzymatic activity Aspergillus terreus
3.2.1.22 Cu2+ inhibits enzymatic activity Aspergillus terreus
3.2.1.22 Hg2+ inhibits enzymatic activity Aspergillus terreus
3.2.1.22 SDS inhibits enzymatic activity Aspergillus terreus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.22 50000
-
 SDS-PAGE Aspergillus terreus
3.2.1.22 51700
-
 native molecular mass Aspergillus terreus
3.2.1.22 72300
-
 SDS-PAGE Aspergillus terreus
3.2.1.22 77300
-
 gel filtration Aspergillus terreus
3.2.1.22 350000
-
 gel filtration Aspergillus terreus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.22 Aspergillus terreus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.22 4-nitrophenyl-alpha-D-galactopyranoside + H2O
-
 Aspergillus terreus 4-nitrophenol + alpha-D-galactopyranose
-
 ?
3.2.1.22 guar gum + H2O
-
 Aspergillus terreus ?
-
 ?
3.2.1.22 locust bean gum + H2O
-
 Aspergillus terreus ?
-
 ?
3.2.1.22 melibiose + H2O
-
 Aspergillus terreus alpha-D-galactose + alpha-D-glucose
-
 ?
3.2.1.22 raffinose + H2O
-
 Aspergillus terreus sucrose + alpha-D-galactose
-
 ?
3.2.1.22 stachyose + 2 H2O
-
 Aspergillus terreus 2 alpha-D-galactose + sucrose
-
 ?
3.2.1.22 sucrose + H2O
-
 Aspergillus terreus alpha-D-glucose + D-fructose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.22 homopentamer 5 * 72300, SDS-PAGE Aspergillus terreus
3.2.1.22 monomer 1 * 50000, SDS-PAGE Aspergillus terreus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.22 E1 alpha-galactosidase
-
 Aspergillus terreus
3.2.1.22 E2 alpha-galactosidase
-
 Aspergillus terreus
3.2.1.22 E3 alpha-galactosidase
-
 Aspergillus terreus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.22 40
-
 assay at Aspergillus terreus
3.2.1.22 50
-
 optimal temperature Aspergillus terreus
3.2.1.22 55
-
 optimal temperature Aspergillus terreus
3.2.1.22 60
-
 optimal temperature Aspergillus terreus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.22 50
-
 retains 90% of its initial activity after 70 h at 50°C Aspergillus terreus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.22 5
-
-
 Aspergillus terreus
3.2.1.22 5
-
 assay at Aspergillus terreus
3.2.1.22 5.5
-
 highest activity Aspergillus terreus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.22 4 7 stable for 6 h Aspergillus terreus