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Literature summary extracted from

  • Eprintsev, A.; Dyachenko, E.; Lykova, T.; Kuen, C.; Popov, V.
    Preparation and properties of isocitrate lyase isoforms from the cotyledons of Glycine max L (2010), Appl. Biochem. Microbiol., 46, 93-98.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.3.1 0.016
-
 isocitrate pH 7.5, 25°C Glycine max
4.1.3.1 0.05
-
 isocitrate pH 7.5, 25°C Glycine max

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.1.3.1 glyoxysome
-
 Glycine max 9514
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.3.1 66000
-
 4 * 66000, SDS-PAGE, gel filtration Glycine max
4.1.3.1 246000
-
 gel filtration Glycine max
4.1.3.1 272000
-
 gel filtration Glycine max

Organism

EC Number Organism UniProt Comment Textmining
4.1.3.1 Glycine max
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.3.1 ammonium sulfate frationation, gel filtration, ion exchange chromatography (DEAE-cellulose) Glycine max

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.3.1 cotyledon
-
 Glycine max
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.3.1 5.28
-
 pH 7.5, 25°C Glycine max
4.1.3.1 5.81
-
 pH 7.5, 25°C Glycine max

Storage Stability

EC Number Storage Stability Organism
4.1.3.1 -20°C, Tris-HCl-buffer, pH 7.5, 50% loss of activity after 3 days, 100% loss of activity after 6 days Glycine max
4.1.3.1 -70°C, Tris-HCl-buffer, pH 7.5, 5% loss of activity after 1 day, 10% loss of activity for each following day Glycine max
4.1.3.1 -70°C, Tris-HCl-buffer, pH 7.5, 80% activity by the tenth day of storage in the presence of 5 mM Mg2+ and 25% of activity when 5 mM Mn2+ are used for stabilization, the following storage conditions are optimal: low temperature (-70°C), 25% glycerol in the medium and the presence of 5 mM MgCl2 Glycine max
4.1.3.1 -70°C, Tris-HCl-buffer, pH 7.5, 85% activity by the tenth day of storage in the presence of 5 mM Mn2+ and 30% of activity when 5 mM Mg2+ are used for stabilization, the following storage conditions are optimal: low temperature (-70°C), 25% glycerol in the medium and the presence of 5 mM MnCl2 Glycine max
4.1.3.1 room temperature, Tris-HCl-buffer, pH 7.5, 80% loss of activity in 24 hours Glycine max
4.1.3.1 storage in glycerol-containing medium (25% glycerol) results in a 30% higher activity than storage in the absence of glycerol Glycine max

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.3.1 isocitrate
-
 Glycine max glyoxylate + succinate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.3.1 homotetramer 4 * 66000, SDS-PAGE, gel filtration Glycine max

Synonyms

EC Number Synonyms Comment Organism
4.1.3.1 isocitrate lyase 1 relative electrophoretic mobility Rf 0.28 Glycine max
4.1.3.1 isocitrate lyase 2 relative electrophoretic mobility Rf 0.44 Glycine max

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.3.1 6.5
-
-
 Glycine max
4.1.3.1 8
-
-
 Glycine max