EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
4.2.1.30 | coenzyme B12 | GDHt utilizes adenosylcobalamin, i.e. AdoCbl or CoB12, as a cofactor, Km is 10.03 nM | Klebsiella pneumoniae |
EC Number | Cloned (Comment) | Organism |
---|---|---|
4.2.1.30 | genes dhaBCE, recombinant expression of His6-tagged GDHt in Escherichia coli strain Bl21(DE3), optimization of IPTG inducting process for recombinant GDHt to increase specific activity and ratio of soluble form using 0.8 mM IPTG at 20°C for 3 h, method development, overview | Klebsiella pneumoniae |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.1.30 | 0.48 | - |
glycerol | pH 8.0, 37°C | Klebsiella pneumoniae | |
4.2.1.30 | 1.43 | - |
1,2-propanediol | pH 8.0, 37°C | Klebsiella pneumoniae | |
4.2.1.30 | 3.07 | - |
1,2-ethanediol | pH 8.0, 37°C | Klebsiella pneumoniae |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.1.30 | Klebsiella pneumoniae | A6NA24 | large subunit, gene dhaB; gene dhaB | - |
4.2.1.30 | Klebsiella pneumoniae | A6NA25 | medium subunit, gene dhaC; gene dhaC | - |
4.2.1.30 | Klebsiella pneumoniae | Q59475 | gamma subunit, gene dhaB3 or dhaE; gene dhaB3 or dhaE | - |
4.2.1.30 | Klebsiella pneumoniae XJPD-Li | A6NA24 | large subunit, gene dhaB; gene dhaB | - |
4.2.1.30 | Klebsiella pneumoniae XJPD-Li | A6NA25 | medium subunit, gene dhaC; gene dhaC | - |
4.2.1.30 | Klebsiella pneumoniae XJPD-Li | Q59475 | gamma subunit, gene dhaB3 or dhaE; gene dhaB3 or dhaE | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.2.1.30 | recombinant His-tagged GDHt 2.11fold by nickel affinity chromatography and ultrafiltration | Klebsiella pneumoniae |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.1.30 | 1,2-ethanediol | - |
Klebsiella pneumoniae | acetaldehyde + H2O | - |
? | |
4.2.1.30 | 1,2-ethanediol | - |
Klebsiella pneumoniae XJPD-Li | acetaldehyde + H2O | - |
? | |
4.2.1.30 | 1,2-propanediol | - |
Klebsiella pneumoniae | propionaldehyde + H2O | - |
? | |
4.2.1.30 | 1,2-propanediol | - |
Klebsiella pneumoniae XJPD-Li | propionaldehyde + H2O | - |
? | |
4.2.1.30 | glycerol | - |
Klebsiella pneumoniae | 3-hydroxypropanal + H2O | - |
? | |
4.2.1.30 | glycerol | - |
Klebsiella pneumoniae XJPD-Li | 3-hydroxypropanal + H2O | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.2.1.30 | GDHt | - |
Klebsiella pneumoniae |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.2.1.30 | 45 | - |
recombinant enzyme | Klebsiella pneumoniae |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.2.1.30 | 37 | 45 | assay range | Klebsiella pneumoniae |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.2.1.30 | 8 | - |
recombinant enzyme | Klebsiella pneumoniae |
EC Number | General Information | Comment | Organism |
---|---|---|---|
4.2.1.30 | metabolism | GDHt is the key enzyme and catalyzes the rate limiting step for biotransformation from glycerol to 1,3-propanediol | Klebsiella pneumoniae |