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Literature summary extracted from

  • Xu, X.; Zhang, G.; Lv, B.; Yuan, Y.; Li, C.
    Recombinant glycerol dehydratase from Klebsiella pneumonia XJPD-Li: induction optimization, purification and characterization (2011), Appl. Biochem. Microbiol., 47, 144-150.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.1.30 coenzyme B12 GDHt utilizes adenosylcobalamin, i.e. AdoCbl or CoB12, as a cofactor, Km is 10.03 nM Klebsiella pneumoniae

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.30 genes dhaBCE, recombinant expression of His6-tagged GDHt in Escherichia coli strain Bl21(DE3), optimization of IPTG inducting process for recombinant GDHt to increase specific activity and ratio of soluble form using 0.8 mM IPTG at 20°C for 3 h, method development, overview Klebsiella pneumoniae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.30 0.48
-
glycerol pH 8.0, 37°C Klebsiella pneumoniae
4.2.1.30 1.43
-
1,2-propanediol pH 8.0, 37°C Klebsiella pneumoniae
4.2.1.30 3.07
-
1,2-ethanediol pH 8.0, 37°C Klebsiella pneumoniae

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.30 Klebsiella pneumoniae A6NA24 large subunit, gene dhaB; gene dhaB
-
4.2.1.30 Klebsiella pneumoniae A6NA25 medium subunit, gene dhaC; gene dhaC
-
4.2.1.30 Klebsiella pneumoniae Q59475 gamma subunit, gene dhaB3 or dhaE; gene dhaB3 or dhaE
-
4.2.1.30 Klebsiella pneumoniae XJPD-Li A6NA24 large subunit, gene dhaB; gene dhaB
-
4.2.1.30 Klebsiella pneumoniae XJPD-Li A6NA25 medium subunit, gene dhaC; gene dhaC
-
4.2.1.30 Klebsiella pneumoniae XJPD-Li Q59475 gamma subunit, gene dhaB3 or dhaE; gene dhaB3 or dhaE
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.30 recombinant His-tagged GDHt 2.11fold by nickel affinity chromatography and ultrafiltration Klebsiella pneumoniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.30 1,2-ethanediol
-
Klebsiella pneumoniae acetaldehyde + H2O
-
?
4.2.1.30 1,2-ethanediol
-
Klebsiella pneumoniae XJPD-Li acetaldehyde + H2O
-
?
4.2.1.30 1,2-propanediol
-
Klebsiella pneumoniae propionaldehyde + H2O
-
?
4.2.1.30 1,2-propanediol
-
Klebsiella pneumoniae XJPD-Li propionaldehyde + H2O
-
?
4.2.1.30 glycerol
-
Klebsiella pneumoniae 3-hydroxypropanal + H2O
-
?
4.2.1.30 glycerol
-
Klebsiella pneumoniae XJPD-Li 3-hydroxypropanal + H2O
-
?

Synonyms

EC Number Synonyms Comment Organism
4.2.1.30 GDHt
-
Klebsiella pneumoniae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.30 45
-
recombinant enzyme Klebsiella pneumoniae

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.2.1.30 37 45 assay range Klebsiella pneumoniae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.30 8
-
recombinant enzyme Klebsiella pneumoniae

General Information

EC Number General Information Comment Organism
4.2.1.30 metabolism GDHt is the key enzyme and catalyzes the rate limiting step for biotransformation from glycerol to 1,3-propanediol Klebsiella pneumoniae